WEKO3
アイテム
Multiphoton microscopy observations of 3D elastin and collagen fiber microstructure changes during pressurization in aortic media
http://hdl.handle.net/2237/26876
http://hdl.handle.net/2237/26876ea079193-0e05-4fdd-9ec0-a80f2c20b85e
名前 / ファイル | ライセンス | アクション |
---|---|---|
SugitaBMMB17rep.pdf ファイル公開:2018/06/01 (1.8 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-08-03 | |||||
タイトル | ||||||
タイトル | Multiphoton microscopy observations of 3D elastin and collagen fiber microstructure changes during pressurization in aortic media | |||||
言語 | en | |||||
著者 |
Sugita, Shukei
× Sugita, Shukei× Matsumoto, Takeo |
|||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | The final publication is available at Springer via https://doi.org/10.1007/s10237-016-0851-9 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Thoracic aorta | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Waviness | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Second harmonic generation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Two-photon microscopy | |||||
抄録 | ||||||
内容記述 | Elastin and collagen fibers play important roles in the mechanical properties of aortic media. Because knowledge of local fiber structures is required for detailed analysis of blood vessel wall mechanics, we investigated 3D microstructures of elastin and collagen fibers in thoracic aortas and monitored changes during pressurization. Using multiphoton microscopy, autofluorescence images from elastin and second harmonic generation signals from collagen were acquired in media from rabbit thoracic aortas that were stretched biaxially to restore physiological dimensions. Both elastin and collagen fibers were observed in all longitudinal–circumferential plane images, whereas alternate bright and dark layers were observed along the radial direction and were recognized as elastic laminas (ELs) and smooth muscle-rich layers (SMLs), respectively. Elastin and collagen fibers are mainly oriented in the circumferential direction, and waviness of collagen fibers was significantly higher than that of elastin fibers. Collagen fibers were more undulated in longitudinal than in radial direction, whereas undulation of elastin fibers was equibiaxial. Changes in waviness of collagen fibers during pressurization were then evaluated using 2-dimensional fast Fourier transform in mouse aortas, and indices of waviness of collagen fibers decreased with increases in intraluminal pressure. These indices also showed that collagen fibers in SMLs became straight at lower intraluminal pressures than those in EL, indicating that SMLs stretched more than ELs. These results indicate that deformation of the aorta due to pressurization is complicated because of the heterogeneity of tissue layers and differences in elastic properties of ELs, SMLs, and surrounding collagen and elastin. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Springer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s10237-016-0851-9 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1617-7959 | |||||
書誌情報 |
en : Biomechanics and Modeling in Mechanobiology 巻 16, 号 3, p. 763-773, 発行日 2017-06 |
|||||
著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | https://doi.org/10.1007/s10237-016-0851-9 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/26876 | |||||
識別子タイプ | HDL |