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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM

http://hdl.handle.net/2237/26958
d0db068e-3a67-4d06-bdf9-5bc68289ffb2
名前 / ファイル ライセンス アクション
Figure9.pdf Figure9.pdf ファイル公開:2018/08/01 (4.8 MB)
Figure8.pdf Figure8.pdf ファイル公開:2018/08/01 (1.2 MB)
Figure7.pdf Figure7.pdf ファイル公開:2018/08/01 (3.8 MB)
Figure6.pdf Figure6.pdf ファイル公開:2018/08/01 (8.8 MB)
Figure5.pdf Figure5.pdf ファイル公開:2018/08/01 (1.5 MB)
Figure4.pdf Figure4.pdf ファイル公開:2018/08/01 (1.0 MB)
Figure3.pdf Figure3.pdf ファイル公開:2018/08/01 (6.2 MB)
Figure2.pdf Figure2.pdf ファイル公開:2018/08/01 (9.1 MB)
Figure1.pdf Figure1.pdf ファイル公開:2018/08/01 (5.3 MB)
Nagoya_Repository-Manuscript.pdf Nagoya_Repository-Manuscript.pdf ファイル公開:2018/08/01 (717.5 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-09-05
タイトル
タイトル Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM
著者 Tamada, Hiromi

× Tamada, Hiromi

WEKO 73445

Tamada, Hiromi

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Kiryu-Seo, Sumiko

× Kiryu-Seo, Sumiko

WEKO 73446

Kiryu-Seo, Sumiko

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Hosokawa, Hiroki

× Hosokawa, Hiroki

WEKO 73447

Hosokawa, Hiroki

Search repository
Ohta, Keisuke

× Ohta, Keisuke

WEKO 73448

Ohta, Keisuke

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Ishihara, Naotada

× Ishihara, Naotada

WEKO 73449

Ishihara, Naotada

Search repository
Nomura, Masatoshi

× Nomura, Masatoshi

WEKO 73450

Nomura, Masatoshi

Search repository
Mihara, Katsuyoshi

× Mihara, Katsuyoshi

WEKO 73451

Mihara, Katsuyoshi

Search repository
Nakamura, Kei-ichiro

× Nakamura, Kei-ichiro

WEKO 73452

Nakamura, Kei-ichiro

Search repository
Kiyama, Hiroshi

× Kiyama, Hiroshi

WEKO 73453

Kiyama, Hiroshi

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権利
権利情報 "This is the peer reviewed version of the following article: [Tamada H, Kiryu-Seo S, Hosokawa H, et al. Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM. J Comp Neurol. 2017;525:2535–2548. https://doi.org/10.1002/cne.24213], which has been published in final form at [https://doi.org/10.1002/cne.24213]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
抄録
内容記述 Mitochondria undergo morphological changes through fusion and fission for their quality control, which are vital for neuronal function. In this study, we examined three-dimensional morphologies of mitochondria in motor neurons under normal, nerve injured, and nerve injured plus fission-impaired conditions using the focused ion beam/scanning electron microscopy (FIB/SEM), because the FIB/SEM technology is a powerful tool to demonstrate both 3D images of whole organelle and the intra-organellar structure simultaneously. Crossing of dynamin-related protein 1 (Drp1) gene-floxed mice with neuronal injury-specific Cre driver mice, Atf3:BAC Tg mice, allowed for Drp1 ablation specifically in injured neurons. FIB/SEM analysis demonstrated that somatic mitochondrial morphologies in motor neurons were not altered before or after nerve injury. However, the fission impairment resulted in prominent somatic mitochondrial enlargement, which initially induced complex morphologies with round regions and long tubular processes, subsequently causing a decrease in the number of processes and further enlargement of the round regions, which eventually resulted in big spheroidal mitochondria without processes. The abnormal mitochondria exhibited several degradative morphologies: local or total cristae collapse, vacuolization, and mitophagy. These suggest that mitochondrial fission is crucial for maintaining mitochondrial integrity in injured motor neurons, and multiple forms of mitochondria degradation may accelerate neuronal degradation.
内容記述タイプ Abstract
出版者
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0021-9967
書誌情報 Journal of Comparative Neurology

巻 525, 号 11, p. 2535-2548, 発行日 2017-08-01
著者版フラグ
値 author
URI
識別子 http://doi.org/10.1002/cne.24213
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/26958
識別子タイプ HDL
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