ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "4acdc7bc-84b7-4ab4-9e77-81371d4c166a"}, "_deposit": {"id": "24806", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24806"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024806", "sets": ["501"]}, "author_link": ["73761", "73762", "73763", "73764", "73765", "73766", "73767", "73768", "73769", "73770"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-06-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "E481", "bibliographicPageStart": "E471", "bibliographicVolumeNumber": "312", "bibliographic_titles": [{"bibliographic_title": "American Journal of Physiology - Endocrinology and Metabolism", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "S100 calcium-binding protein B (S100B), a multifunctional macromolecule mainly expressed in nerve tissues and adipocytes, has been suggested to contribute to the pathogenesis of obesity. To clarify the role of S100B in insulin action and glucose metabolism in peripheral tissues, we investigated the effect of S100B on glycolysis in myoblast and myotube cells. Rat myoblast L6 cells were treated with recombinant mouse S100B to examine glucose consumption, lactate production, glycogen accumulation, glycolytic metabolites and enzyme activity, insulin signaling, and poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Glycolytic metabolites were investigated by enzyme assays or metabolome analysis, and insulin signaling was assessed by Western blot analysis. Enzyme activity and poly(ADP-ribosyl)ation of GAPDH was evaluated by an enzyme assay and immunoprecipitation followed by dot blot with an anti-poly(ADP-ribose) antibody, respectively. S100B significantly decreased glucose consumption, glucose analog uptake, and lactate production in L6 cells, in either the presence or absence of insulin. In contrast, S100B had no effect on glycogen accumulation and insulin signaling. Metabolome analysis revealed that S100B increased the concentration of glycolytic intermediates upstream of GAPDH. S100B impaired GAPDH activity and increased poly(ADP-ribosyl)ated GAPDH proteins. The effects of S100B on glucose metabolism were mostly canceled by a poly(ADP-ribose) polymerase inhibitor. Similar results were obtained in C2C12 myotube cells. We conclude that S100B as a humoral factor may impair glycolysis in muscle cells independent of insulin action, and the effect may be attributed to the inhibition of GAPDH activity from enhanced poly(ADP-ribosyl)ation of the enzyme.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1152/ajpendo.00328.2016"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27028"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Physiological Society", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1152/ajpendo.00328.2016", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright (c) 2017 the American Physiological Society", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0193-1849", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hosokawa, Kaori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73761", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hamada, Yoji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73762", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujiya, Atsushi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73763", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murase, Masatoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73764", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maekawa, Ryuya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73765", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Niwa, Yasuhiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73766", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Izumoto, Takako", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73767", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Seino, Yusuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73768", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsunekawa, Shin", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73769", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Arima, Hiroshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73770", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AJP_revision_figs_acceptPDF.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "AJP_revision_figs_acceptPDF.pdf ファイル公開:2018/06/01", "objectType": "dataset", "url": "https://nagoya.repo.nii.ac.jp/record/24806/files/AJP_revision_figs_acceptPDF.pdf"}, "version_id": "92c75e5b-0605-4a80-a581-d6225469160d"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "AJPaccept_verPDF.pdf", "filesize": [{"value": "214.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 214300.0, "url": {"label": "AJPaccept_verPDF.pdf ファイル公開:2018/06/01", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/24806/files/AJPaccept_verPDF.pdf"}, "version_id": "377043b0-97bc-4e8e-bc98-19250be8d699"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "S100B impairs glycolysis via enhanced poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase in rodent muscle cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "S100B impairs glycolysis via enhanced poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase in rodent muscle cells", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["501"], "permalink_uri": "http://hdl.handle.net/2237/27028", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-10-10"}, "publish_date": "2017-10-10", "publish_status": "0", "recid": "24806", "relation": {}, "relation_version_is_last": true, "title": ["S100B impairs glycolysis via enhanced poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase in rodent muscle cells"], "weko_shared_id": -1}
  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

S100B impairs glycolysis via enhanced poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase in rodent muscle cells

http://hdl.handle.net/2237/27028
http://hdl.handle.net/2237/27028
a3529229-fed6-451d-bee8-d276f0cc54f8
名前 / ファイル ライセンス アクション
AJP_revision_figs_acceptPDF.pdf AJP_revision_figs_acceptPDF.pdf ファイル公開:2018/06/01 (1.1 MB)
AJPaccept_verPDF.pdf AJPaccept_verPDF.pdf ファイル公開:2018/06/01 (214.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-10-10
タイトル
タイトル S100B impairs glycolysis via enhanced poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase in rodent muscle cells
言語 en
著者 Hosokawa, Kaori

× Hosokawa, Kaori

WEKO 73761

en Hosokawa, Kaori

Search repository
Hamada, Yoji

× Hamada, Yoji

WEKO 73762

en Hamada, Yoji

Search repository
Fujiya, Atsushi

× Fujiya, Atsushi

WEKO 73763

en Fujiya, Atsushi

Search repository
Murase, Masatoshi

× Murase, Masatoshi

WEKO 73764

en Murase, Masatoshi

Search repository
Maekawa, Ryuya

× Maekawa, Ryuya

WEKO 73765

en Maekawa, Ryuya

Search repository
Niwa, Yasuhiro

× Niwa, Yasuhiro

WEKO 73766

en Niwa, Yasuhiro

Search repository
Izumoto, Takako

× Izumoto, Takako

WEKO 73767

en Izumoto, Takako

Search repository
Seino, Yusuke

× Seino, Yusuke

WEKO 73768

en Seino, Yusuke

Search repository
Tsunekawa, Shin

× Tsunekawa, Shin

WEKO 73769

en Tsunekawa, Shin

Search repository
Arima, Hiroshi

× Arima, Hiroshi

WEKO 73770

en Arima, Hiroshi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 Copyright (c) 2017 the American Physiological Society
抄録
内容記述 S100 calcium-binding protein B (S100B), a multifunctional macromolecule mainly expressed in nerve tissues and adipocytes, has been suggested to contribute to the pathogenesis of obesity. To clarify the role of S100B in insulin action and glucose metabolism in peripheral tissues, we investigated the effect of S100B on glycolysis in myoblast and myotube cells. Rat myoblast L6 cells were treated with recombinant mouse S100B to examine glucose consumption, lactate production, glycogen accumulation, glycolytic metabolites and enzyme activity, insulin signaling, and poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Glycolytic metabolites were investigated by enzyme assays or metabolome analysis, and insulin signaling was assessed by Western blot analysis. Enzyme activity and poly(ADP-ribosyl)ation of GAPDH was evaluated by an enzyme assay and immunoprecipitation followed by dot blot with an anti-poly(ADP-ribose) antibody, respectively. S100B significantly decreased glucose consumption, glucose analog uptake, and lactate production in L6 cells, in either the presence or absence of insulin. In contrast, S100B had no effect on glycogen accumulation and insulin signaling. Metabolome analysis revealed that S100B increased the concentration of glycolytic intermediates upstream of GAPDH. S100B impaired GAPDH activity and increased poly(ADP-ribosyl)ated GAPDH proteins. The effects of S100B on glucose metabolism were mostly canceled by a poly(ADP-ribose) polymerase inhibitor. Similar results were obtained in C2C12 myotube cells. We conclude that S100B as a humoral factor may impair glycolysis in muscle cells independent of insulin action, and the effect may be attributed to the inhibition of GAPDH activity from enhanced poly(ADP-ribosyl)ation of the enzyme.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 American Physiological Society
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1152/ajpendo.00328.2016
ISSN
収録物識別子タイプ PISSN
収録物識別子 0193-1849
書誌情報 en : American Journal of Physiology - Endocrinology and Metabolism

巻 312, 号 6, p. E471-E481, 発行日 2017-06-01
著者版フラグ
値 author
URI
識別子 http://doi.org/10.1152/ajpendo.00328.2016
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/27028
識別子タイプ HDL
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 13:53:06.919894
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3