{"_buckets": {"deposit": "0ff72c84-6e5c-4ed7-9798-1a35eef22ac6"}, "_deposit": {"id": "24853", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24853"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024853"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-08", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "307", "bibliographicPageStart": "304", "bibliographicVolumeNumber": "471", "bibliographic_titles": [{"bibliographic_title": "Clinica Chimica Acta"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Background: Anti-Su antibodies are found in 5\u201320% of cases of various systemic autoimmune rheumatic diseases\nand in 5\u201310% of dermatomyositis (DM)/polymyositis (PM) patients. In 2006, the 100 kDa Su antigen was\nidentified as argonaute2 (Ago2), and it was found to play a major role in RNA interference. However, immunoprecipitation\n(IP) remains the main method for detecting anti-Su and the clinical significance of the antibodies\nis uncertain.\nMethods: Sera from patients with DM/PM (n = 224) were screened by an ELISA that uses recombinant biotinylated\nAgo2 protein. Some serum samples were tested by IP and by indirect immunofluorescence (IIF) analysis.\nResults: Seventeen (7.5%, 17/224) sera from DM/PM were positive in ELISA. Of the 33 IP-tested sera (17 ELISApositive\nand 16 ELISA-negative with high background), 13 were found to be anti-Ago2/Su-positive in IP and\nELISA. Only one IP-positive serum was judged to be ELISA-negative. Among the 13 patients with anti-Su/Ago2, 7\ncases also had myositis-specific autoantibodies. Six sera that were positive by both IP and ELISA showed the GW\nbody pattern in IIF. Interestingly, one ELISA-positive serum with an inconclusive result in IP also showed the GW\nbody pattern.\nConclusion: Our novel ELISA appears to be useful for screening anti-Su/Ago2 antibodies (sensitivity: 93%,\nspecificity: 79%).", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1016/j.cca.2017.06.022"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27073"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0009-8981", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mariko, Ogawa-Momohara"}], "nameIdentifiers": [{"nameIdentifier": "73920", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshinao, Muro"}], "nameIdentifiers": [{"nameIdentifier": "73921", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Minoru, Satoh"}], "nameIdentifiers": [{"nameIdentifier": "73922", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Masashi, Akiyama"}], "nameIdentifiers": [{"nameIdentifier": "73923", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-08-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CCA-D-17-00162R2_1.pdf", "filesize": [{"value": "372.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 372700.0, "url": {"label": "CCA-D-17-00162R2_1.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5:2018/08/01", "url": "https://nagoya.repo.nii.ac.jp/record/24853/files/CCA-D-17-00162R2_1.pdf"}, "version_id": "7215c4d3-f7fc-49bb-a64a-cf269468e5e5"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Autoantibodies to Su/Argonaute 2 in Japanese patients with inflammatory myopathy", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Autoantibodies to Su/Argonaute 2 in Japanese patients with inflammatory myopathy"}]}, "item_type_id": "10", "owner": "1", "path": ["499/500/501"], "permalink_uri": "http://hdl.handle.net/2237/27073", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2017-11-07"}, "publish_date": "2017-11-07", "publish_status": "0", "recid": "24853", "relation": {}, "relation_version_is_last": true, "title": ["Autoantibodies to Su/Argonaute 2 in Japanese patients with inflammatory myopathy"], "weko_shared_id": null}