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Autoantibodies to Su/Argonaute 2 in Japanese patients with inflammatory myopathy
http://hdl.handle.net/2237/27073
http://hdl.handle.net/2237/2707386febf11-c023-4cf8-9025-d47e602c2678
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CCA-D-17-00162R2_1.pdf ファイル公開日:2018/08/01 (372.7 kB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-11-07 | |||||
| タイトル | ||||||
| タイトル | Autoantibodies to Su/Argonaute 2 in Japanese patients with inflammatory myopathy | |||||
| 言語 | en | |||||
| 著者 |
Mariko, Ogawa-Momohara
× Mariko, Ogawa-Momohara× Yoshinao, Muro× Minoru, Satoh× Masashi, Akiyama |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 権利情報 | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 言語 | en | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Anti-Su antibodies are found in 5–20% of cases of various systemic autoimmune rheumatic diseases and in 5–10% of dermatomyositis (DM)/polymyositis (PM) patients. In 2006, the 100 kDa Su antigen was identified as argonaute2 (Ago2), and it was found to play a major role in RNA interference. However, immunoprecipitation (IP) remains the main method for detecting anti-Su and the clinical significance of the antibodies is uncertain. Methods: Sera from patients with DM/PM (n = 224) were screened by an ELISA that uses recombinant biotinylated Ago2 protein. Some serum samples were tested by IP and by indirect immunofluorescence (IIF) analysis. Results: Seventeen (7.5%, 17/224) sera from DM/PM were positive in ELISA. Of the 33 IP-tested sera (17 ELISApositive and 16 ELISA-negative with high background), 13 were found to be anti-Ago2/Su-positive in IP and ELISA. Only one IP-positive serum was judged to be ELISA-negative. Among the 13 patients with anti-Su/Ago2, 7 cases also had myositis-specific autoantibodies. Six sera that were positive by both IP and ELISA showed the GW body pattern in IIF. Interestingly, one ELISA-positive serum with an inconclusive result in IP also showed the GW body pattern. Conclusion: Our novel ELISA appears to be useful for screening anti-Su/Ago2 antibodies (sensitivity: 93%, specificity: 79%). | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.cca.2017.06.022 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0009-8981 | |||||
| 書誌情報 |
en : Clinica Chimica Acta 巻 471, p. 304-307, 発行日 2017-08 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://doi.org/10.1016/j.cca.2017.06.022 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/27073 | |||||
| 識別子タイプ | HDL | |||||
