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Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab
http://hdl.handle.net/2237/27076
http://hdl.handle.net/2237/27076e8c69cb3-f10c-4854-9f7f-37e78c1ca3ec
名前 / ファイル | ライセンス | アクション |
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TSenarai_FinalMRD2017.pdf ファイル公開日:2018/07/01 (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-11-07 | |||||
タイトル | ||||||
タイトル | Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab | |||||
言語 | en | |||||
著者 |
yaporn, Senarai
× yaporn, Senarai× Rapeepun, Vanichviriyakit× Shinji, Miyata× Chihiro, Sato× Prapee, Sretarugsa× Wattana, Weerachatyanukul× Ken, Kitajima |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is the peer reviewed version of the following article: [MOLECULAR REPRODUCTION AND DEVELOPMENT. v.84, n.7, 2017, p.585-595], which has been published in final form at [http://doi.org/10.1002/mrd.22817]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | crab sperm | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | male reproductive tract | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protease inhibitor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | seminal fluid protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sperm maturation | |||||
抄録 | ||||||
内容記述 | A 250-kDa protein was isolated from fluid in the middle spermatic duct (MSD) of the blue crab (Portunus pelagicus). N-terminal and partial amino acid sequences revealed that this MSD-specific protein is highly similar to the plasma-enriched protein Alpha-2 macroglobulin (α2M). The P. pelagicus ortholog (Ppα2M) is a large glycoprotein possessing mannose and N-acetylglucosamine residues. Ppa2m mRNA was detected in the spermatic duct, androgenic gland, and hematopoietic tissue, whereas the protein was primarily observed in the apical cytoplasm of MSD epithelium and in the matrix of the acrosome of MSD sperm; distally within spermatic duct, Ppα2M was lost from the sperm membrane but remained in the sperm acrosome. These results suggest that Ppα2M is expressed and glycosylated in the epithelium of spermatic ducts, secreted into MSD fluid, taken up by sperm in the MSD, and removed from the surface of sperm during its transit towards the female spermatheca. Given that Ppα2M also exhibits protease inhibitor activity, we hypothesize that acrosome localized Ppα2M may suppress premature acrosome reaction during post-testicular sperm maturation in this crab. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Wiley | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1002/mrd.22817 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1040-452X | |||||
書誌情報 |
en : Molecular Reproduction and Development 巻 84, 号 7, p. 585-595, 発行日 2017-07 |
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著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://doi.org/10.1002/mrd.22817 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/27076 | |||||
識別子タイプ | HDL |