{"_buckets": {"deposit": "9a5fc24d-bb87-4a2d-8332-7011d1323c3d"}, "_deposit": {"id": "24856", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24856"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024856"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "7", "bibliographicPageEnd": "595", "bibliographicPageStart": "585", "bibliographicVolumeNumber": "84", "bibliographic_titles": [{"bibliographic_title": "Molecular Reproduction and Development"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "A 250-kDa protein was isolated from fluid in the middle spermatic duct (MSD) of the blue crab (Portunus pelagicus). N-terminal and partial amino acid sequences revealed that this MSD-specific protein is highly similar to the plasma-enriched protein Alpha-2 macroglobulin (\u03b12M). The P. pelagicus ortholog (Pp\u03b12M) is a large glycoprotein possessing mannose and N-acetylglucosamine residues. Ppa2m mRNA was detected in the spermatic duct, androgenic gland, and hematopoietic tissue, whereas the protein was primarily observed in the apical cytoplasm of MSD epithelium and in the matrix of the acrosome of MSD sperm; distally within spermatic duct, Pp\u03b12M was lost from the sperm membrane but remained in the sperm acrosome. These results suggest that Pp\u03b12M is expressed and glycosylated in the epithelium of spermatic ducts, secreted into MSD fluid, taken up by sperm in the MSD, and removed from the surface of sperm during its transit towards the female spermatheca. Given that Pp\u03b12M also exhibits protease inhibitor activity, we hypothesize that acrosome localized Pp\u03b12M may suppress premature acrosome reaction during post-testicular sperm maturation in this crab.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1002/mrd.22817"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27076"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Wiley"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\"This is the peer reviewed version of the following article: [MOLECULAR REPRODUCTION AND DEVELOPMENT. v.84, n.7, 2017, p.585-595], which has been published in final form at [http://doi.org/10.1002/mrd.22817]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.\""}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1040-452X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "yaporn, Senarai"}], "nameIdentifiers": [{"nameIdentifier": "73932", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Rapeepun, Vanichviriyakit"}], "nameIdentifiers": [{"nameIdentifier": "73933", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shinji, Miyata"}], "nameIdentifiers": [{"nameIdentifier": "73934", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Chihiro, Sato"}], "nameIdentifiers": [{"nameIdentifier": "73935", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Prapee, Sretarugsa"}], "nameIdentifiers": [{"nameIdentifier": "73936", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wattana, Weerachatyanukul"}], "nameIdentifiers": [{"nameIdentifier": "73937", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ken, Kitajima"}], "nameIdentifiers": [{"nameIdentifier": "73938", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-07-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "TSenarai_FinalMRD2017.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "TSenarai_FinalMRD2017.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5:2018/07/01", "url": "https://nagoya.repo.nii.ac.jp/record/24856/files/TSenarai_FinalMRD2017.pdf"}, "version_id": "3ea6019f-2d00-4874-8e12-bd28ea39b54a"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "crab sperm", "subitem_subject_scheme": "Other"}, {"subitem_subject": "male reproductive tract", "subitem_subject_scheme": "Other"}, {"subitem_subject": "protease inhibitor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "seminal fluid protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sperm maturation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab"}]}, "item_type_id": "10", "owner": "1", "path": ["1179/2033/2034"], "permalink_uri": "http://hdl.handle.net/2237/27076", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-11-07"}, "publish_date": "2017-11-07", "publish_status": "0", "recid": "24856", "relation": {}, "relation_version_is_last": true, "title": ["Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab"], "weko_shared_id": null}