{"_buckets": {"deposit": "ce612738-ce31-46bf-822f-030fa6c8792f"}, "_deposit": {"id": "25018", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25018"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025018", "sets": ["501"]}, "author_link": ["74556", "74557", "74558", "74559", "74560", "74561", "74562", "74563", "74564"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageEnd": "1943", "bibliographicPageStart": "1927", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "GLIA", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Several types of myeloid cell are resident in the CNS. In the steady state, microglia are present in the CNS parenchyma, whereas macrophages reside in boundary regions of the CNS, such as perivascular spaces, the meninges and choroid plexus. In addition, monocytes infiltrate into the CNS parenchyma from circulation upon blood–brain barrier breakdown after CNS injury and inflammation. Although several markers, such as CD11b and ionized calcium-binding adapter molecule 1 (Iba1), are frequently used as microglial markers, they are also expressed by other types of myeloid cell and microglia-specific markers were not defined until recently. Previous transcriptome analyses of isolated microglia identified a transmembrane lectin, sialic acid-binding immunoglobulin-like lectin H (Siglec-H), as a molecular signature for microglia; however, this was not confirmed by histological studies in the nervous system and the reliability of Siglec-H as a microglial marker remained unclear. Here, we demonstrate that Siglec-H is an authentic marker for microglia in mice by immunohistochemistry using a Siglec-H-specific antibody. Siglec-H was expressed by parenchymal microglia from developmental stages to adulthood, and the expression was maintained in activated microglia under injury or inflammatory condition. However, Siglec-H expression was absent from CNS-associated macrophages and CNS-infiltrating monocytes, except for a minor subset of cells. We also show that the Siglech gene locus is a feasible site for specific targeting of microglia in the nervous system. In conclusion, Siglec-H is a reliable marker for microglia that will allow histological identification of microglia and microglia-specific gene manipulation in the nervous system.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1002/glia.23204"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27242"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Wiley", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1002/glia.23204", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is the peer reviewed version of the following article: [Konishi, H; Kobayashi, M; Kunisawa, T; Imai, K; Sayo, A; Malissen, B; Crocker, PR; Sato, K; Kiyama, H. (2017),Siglec-H is a microglia-specific marker that discriminates microglia from CNS-associated macrophages and CNS-infiltrating monocytes. GLIA, 65, 12: 1927-1943. doi:10.1111/jdv.13290], which has been published in final form at [http://doi.org/10.1002/glia.23204]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0894-1491", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Konishi, Hiroyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74556", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Masaaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74557", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kunisawa, Taikan", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74558", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imai, Kenta", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74559", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sayo, Akira", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74560", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Malissen, Bernard", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74561", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Crocker, Paul R.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74562", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Katsuaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74563", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kiyama, Hiroshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74564", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-12-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Konishi_Glia_2017.pdf", "filesize": [{"value": "2.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2400000.0, "url": {"label": "Konishi_Glia_2017.pdf ファイル公開日:2018/12/01", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/25018/files/Konishi_Glia_2017.pdf"}, "version_id": "913a474e-b303-4875-a07f-663d25b96811"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "perivascular spaces", "subitem_subject_scheme": "Other"}, {"subitem_subject": "allodynia", "subitem_subject_scheme": "Other"}, {"subitem_subject": "choroid plexus", "subitem_subject_scheme": "Other"}, {"subitem_subject": "inflammation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "meninges", "subitem_subject_scheme": "Other"}, {"subitem_subject": "myeloid cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "pain", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Siglec-H is a microglia-specific marker that discriminates microglia from CNS-associated macrophages and CNS-infiltrating monocytes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Siglec-H is a microglia-specific marker that discriminates microglia from CNS-associated macrophages and CNS-infiltrating monocytes", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["501"], "permalink_uri": "http://hdl.handle.net/2237/27242", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-01-09"}, "publish_date": "2018-01-09", "publish_status": "0", "recid": "25018", "relation": {}, "relation_version_is_last": true, "title": ["Siglec-H is a microglia-specific marker that discriminates microglia from CNS-associated macrophages and CNS-infiltrating monocytes"], "weko_shared_id": -1}