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Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines
http://hdl.handle.net/2237/27347
http://hdl.handle.net/2237/27347b0f384bf-6149-47af-95bb-2061202efc44
名前 / ファイル | ライセンス | アクション |
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Ohtsu_et_al_2017_PROTOPLSSMA.pdf ファイル公開日:2018/11/01 (20.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-01-31 | |||||
タイトル | ||||||
タイトル | Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines | |||||
言語 | en | |||||
著者 |
Ohtsu, Mina
× Ohtsu, Mina× Sato, Yoshikatsu× Kurihara, Daisuke× Suzaki, Takuya× Kawaguchi, Masayoshi× Maruyama, Daisuke× Higashiyama, Tetsuya |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | The final publication is available at Springer via http://doi.org/10.1007/s00709-017-1105-0 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plant-parasitic nematode | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heterodera | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | syncytium development | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | two-photon excitation microscopy(2PEM) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | deep imaging | |||||
抄録 | ||||||
内容記述 | Parasite infections cause dramatic anatomical and ultrastructural changes in host plants. Cyst nematodes are parasites that invade host roots and induce a specific feeding structure called a syncytium. A syncytium is a large multinucleate cell formed by cell wall dissolution-mediated cell fusion. The soybean cyst nematode (SCN), Heterodera glycines, is a major soybean pathogen. To investigate SCN infection and the syncytium structure, we established an in planta deep imaging system using a clearing solution ClearSee and two-photon excitation microscopy (2PEM). Using this system, we found that several cells were incorporated into the syncytium; the nuclei increased in size and the cell wall openings began to be visible at 2 days after inoculation (DAI). Moreover, at 14 DAI, in the syncytium developed in the cortex, there were thickened concave cell wall pillars that resembled “Parthenon pillars.” In contrast, there were many thick board-like cell walls and rarely Parthenon pillars in the syncytium developed in the stele. We revealed that the syncytia were classified into two types based on the pattern of the cell wall structures, which appeared to be determined by the position of the syncytium inside roots. Our results provide new insights into the developmental process of syncytium induced by cyst nematode and a better understanding of the three-dimensional structure of the syncytium in host roots. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Springer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00709-017-1105-0 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0033-183X | |||||
書誌情報 |
en : PROTOPLASMA 巻 254, 号 6, p. 2107-2115, 発行日 2017-11 |
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著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://doi.org/10.1007/s00709-017-1105-0 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/27347 | |||||
識別子タイプ | HDL |