{"_buckets": {"deposit": "59ac9dd4-04bd-4604-a0f2-001eda7486d4"}, "_deposit": {"id": "25183", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25183"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025183"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-12-15", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "24", "bibliographicPageEnd": "6331", "bibliographicPageStart": "6322", "bibliographicVolumeNumber": "25", "bibliographic_titles": [{"bibliographic_title": "BIOORGANIC \u0026 MEDICINAL CHEMISTRY"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The antitumor and actin-depolymerizing marine macrolide aplyronine A (ApA) synergistically binds to tubulin in association with actin, and prevents spindle formation and mitosis. While the crystal structure of the actin ApA complex was solved in 2006, its interaction with the tubulin heterodimer has not been clarified. To investigate the binding modes of ApA as a unique protein\u2013protein interaction (PPI)-inducer between these two cytoskeletal proteins, we prepared its photoaffinity acetylene and fluorescent derivatives with the aid of molecular modeling studies for probe design. Among these three derivatives, the ApA\u2013PPA\u2013TAMRA probe specifically photoreacted with both actin and tubulin in vitro. However, the photolabeling yield of tubulin was quite low (up to \u223c1%), and one of the major side-reactions was the addition of a water molecule to the carbene species generated from an aryldiazirine moiety on the hydrophilic surface of actin.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1016/j.bmc.2017.09.044"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27403"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0968-0896", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kita, Masaki"}], "nameIdentifiers": [{"nameIdentifier": "75120", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamagishi, Kota"}], "nameIdentifiers": [{"nameIdentifier": "75121", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuchiya, Kota"}], "nameIdentifiers": [{"nameIdentifier": "75122", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Seguchi, Yu"}], "nameIdentifiers": [{"nameIdentifier": "75123", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakane, Hiroki"}], "nameIdentifiers": [{"nameIdentifier": "75124", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kigoshi, Hideo"}], "nameIdentifiers": [{"nameIdentifier": "75125", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-12-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ApA_PF_BMC_MS_r1_f.pdf", "filesize": [{"value": "4.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 4400000.0, "url": {"label": "ApA_PF_BMC_MS_r1_f.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5:2019/12/15", "url": "https://nagoya.repo.nii.ac.jp/record/25183/files/ApA_PF_BMC_MS_r1_f.pdf"}, "version_id": "8fcd92b6-59e4-487b-a0db-c80ef9f0ee19"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Photoaffinity probe", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Protein\u2013protein interaction", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Antitumor natural products", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Actin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tubulin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin"}]}, "item_type_id": "10", "owner": "1", "path": ["643/666/667"], "permalink_uri": "http://hdl.handle.net/2237/27403", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2018-02-13"}, "publish_date": "2018-02-13", "publish_status": "0", "recid": "25183", "relation": {}, "relation_version_is_last": true, "title": ["Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin"], "weko_shared_id": null}