ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "59ac9dd4-04bd-4604-a0f2-001eda7486d4"}, "_deposit": {"id": "25183", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25183"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025183", "sets": ["667"]}, "author_link": ["75120", "75121", "75122", "75123", "75124", "75125"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-12-15", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "24", "bibliographicPageEnd": "6331", "bibliographicPageStart": "6322", "bibliographicVolumeNumber": "25", "bibliographic_titles": [{"bibliographic_title": "BIOORGANIC \u0026 MEDICINAL CHEMISTRY", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The antitumor and actin-depolymerizing marine macrolide aplyronine A (ApA) synergistically binds to tubulin in association with actin, and prevents spindle formation and mitosis. While the crystal structure of the actin ApA complex was solved in 2006, its interaction with the tubulin heterodimer has not been clarified. To investigate the binding modes of ApA as a unique protein–protein interaction (PPI)-inducer between these two cytoskeletal proteins, we prepared its photoaffinity acetylene and fluorescent derivatives with the aid of molecular modeling studies for probe design. Among these three derivatives, the ApA–PPA–TAMRA probe specifically photoreacted with both actin and tubulin in vitro. However, the photolabeling yield of tubulin was quite low (up to ∼1%), and one of the major side-reactions was the addition of a water molecule to the carbene species generated from an aryldiazirine moiety on the hydrophilic surface of actin.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1016/j.bmc.2017.09.044"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27403"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bmc.2017.09.044", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0968-0896", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kita, Masaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "75120", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamagishi, Kota", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "75121", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuchiya, Kota", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "75122", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Seguchi, Yu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "75123", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakane, Hiroki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "75124", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kigoshi, Hideo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "75125", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-12-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ApA_PF_BMC_MS_r1_f.pdf", "filesize": [{"value": "4.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 4400000.0, "url": {"label": "ApA_PF_BMC_MS_r1_f.pdf ファイル公開日:2019/12/15", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/25183/files/ApA_PF_BMC_MS_r1_f.pdf"}, "version_id": "8fcd92b6-59e4-487b-a0db-c80ef9f0ee19"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Photoaffinity probe", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Protein–protein interaction", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Antitumor natural products", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Actin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tubulin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/27403", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-02-13"}, "publish_date": "2018-02-13", "publish_status": "0", "recid": "25183", "relation": {}, "relation_version_is_last": true, "title": ["Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin"], "weko_shared_id": -1}
  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin

http://hdl.handle.net/2237/27403
http://hdl.handle.net/2237/27403
bf7e1a19-1e57-4a35-b54b-f5b3e940b216
名前 / ファイル ライセンス アクション
ApA_PF_BMC_MS_r1_f.pdf ApA_PF_BMC_MS_r1_f.pdf ファイル公開日:2019/12/15 (4.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-02-13
タイトル
タイトル Development of photoaffinity derivatives of the antitumor macrolide aplyronine A, a PPI-inducer between actin and tubulin
言語 en
著者 Kita, Masaki

× Kita, Masaki

WEKO 75120

en Kita, Masaki

Search repository
Yamagishi, Kota

× Yamagishi, Kota

WEKO 75121

en Yamagishi, Kota

Search repository
Tsuchiya, Kota

× Tsuchiya, Kota

WEKO 75122

en Tsuchiya, Kota

Search repository
Seguchi, Yu

× Seguchi, Yu

WEKO 75123

en Seguchi, Yu

Search repository
Nakane, Hiroki

× Nakane, Hiroki

WEKO 75124

en Nakane, Hiroki

Search repository
Kigoshi, Hideo

× Kigoshi, Hideo

WEKO 75125

en Kigoshi, Hideo

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 Photoaffinity probe
キーワード
主題Scheme Other
主題 Protein–protein interaction
キーワード
主題Scheme Other
主題 Antitumor natural products
キーワード
主題Scheme Other
主題 Actin
キーワード
主題Scheme Other
主題 Tubulin
抄録
内容記述 The antitumor and actin-depolymerizing marine macrolide aplyronine A (ApA) synergistically binds to tubulin in association with actin, and prevents spindle formation and mitosis. While the crystal structure of the actin ApA complex was solved in 2006, its interaction with the tubulin heterodimer has not been clarified. To investigate the binding modes of ApA as a unique protein–protein interaction (PPI)-inducer between these two cytoskeletal proteins, we prepared its photoaffinity acetylene and fluorescent derivatives with the aid of molecular modeling studies for probe design. Among these three derivatives, the ApA–PPA–TAMRA probe specifically photoreacted with both actin and tubulin in vitro. However, the photolabeling yield of tubulin was quite low (up to ∼1%), and one of the major side-reactions was the addition of a water molecule to the carbene species generated from an aryldiazirine moiety on the hydrophilic surface of actin.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.bmc.2017.09.044
ISSN
収録物識別子タイプ PISSN
収録物識別子 0968-0896
書誌情報 en : BIOORGANIC & MEDICINAL CHEMISTRY

巻 25, 号 24, p. 6322-6331, 発行日 2017-12-15
著者版フラグ
値 author
URI
識別子 http://doi.org/10.1016/j.bmc.2017.09.044
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/27403
識別子タイプ HDL
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 13:43:36.613424
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3