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D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation
http://hdl.handle.net/2237/00027915
http://hdl.handle.net/2237/000279155a17b8e8-eb02-4a14-a119-4edc9572b8fe
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
J_BiolChem-2017-Nagao-20046-57 (1.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-04-17 | |||||
| タイトル | ||||||
| タイトル | D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation | |||||
| 言語 | en | |||||
| 著者 |
Nagao, Ryo
× Nagao, Ryo× Ueoka-Nakanishi, Hanayo× Noguchi, Takumi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | This research was originally published in the Journal of Biological Chemistry. Ryo Nagao, Hanayo Ueoka-Nakanishi, and Takumi Noguchi. D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation. J. Biol. Chem. 2017; 292:200046-20057. © the American Society for Biochemistry and Molecular Biology or © the Author(s). | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cyanobacteria | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fourier transform IR (FTIR) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | photosynthesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | photosystem II | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | site-directed mutagenesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | D1-Asn298 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | YZ | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | proton trasfer | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | thermoluminescence | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | water oxidation | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In photosynthetic water oxidation, two water molecules are converted into one oxygen molecule and four protons at the Mn4CaO5 cluster in photosystem II (PSII) via the S-state cycle. Efficient proton exit from the catalytic site to the lumen is essential for this process. However, the exit pathways of individual protons through the PSII proteins remain to be identified. In this study, we examined the involvement of a hydrogen-bond network near the redox-active tyrosine YZ in proton transfer during the S-state cycle. We focused on spectroscopic analyses of a site-directed variant of D1-Asn-298, a residue involved in a hydrogen-bond network near YZ. We found that the D1-N298A mutant of Synechocystis sp. PCC 6803 exhibits an O2 evolution activity of ∼10% of the wild-type. D1-N298A and the wild-type D1 had very similar features of thermoluminescence glow curves and of an FTIR difference spectrum upon YZ oxidation, suggesting that the hydrogen-bonded structure of YZ and electron transfer from the Mn4CaO5 cluster to YZ were little affected by substitution. In the D1-N298A mutant, however, the flash-number dependence of delayed luminescence showed a monotonic increase without oscillation, and FTIR difference spectra of the S-state cycle indicated partial and significant inhibition of the S2 → S3 and S3 → S0 transitions, respectively. These results suggest that the D1-N298A substitution inhibits the proton transfer processes in the S2 → S3 and S3 → S0 transitions. This in turn indicates that the hydrogen-bond network near YZ can be functional as a proton transfer pathway during photosynthetic water oxidation. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ファイル公開日: 2018/12/08 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | American Society for Biochemistry and Molecular Biology | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1074/jbc.M117.815183 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0021-9258 | |||||
| 書誌情報 |
en : Journal of Biological Chemistry 巻 292, 号 49, p. 20046-20057, 発行日 2017-12-08 |
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| 著者版フラグ | ||||||
| 値 | publisher | |||||
