{"_buckets": {"deposit": "ce8151ca-2f03-4031-bb58-54bf28f3ed49"}, "_deposit": {"id": "25707", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25707"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025707", "sets": ["696"]}, "author_link": ["76141", "76142", "76143"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-12-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "49", "bibliographicPageEnd": "20057", "bibliographicPageStart": "20046", "bibliographicVolumeNumber": "292", "bibliographic_titles": [{"bibliographic_title": "Journal of Biological Chemistry", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In photosynthetic water oxidation, two water molecules are converted into one oxygen molecule and four protons at the Mn4CaO5 cluster in photosystem II (PSII) via the S-state cycle. Efficient proton exit from the catalytic site to the lumen is essential for this process. However, the exit pathways of individual protons through the PSII proteins remain to be identified. In this study, we examined the involvement of a hydrogen-bond network near the redox-active tyrosine YZ in proton transfer during the S-state cycle. We focused on spectroscopic analyses of a site-directed variant of D1-Asn-298, a residue involved in a hydrogen-bond network near YZ. We found that the D1-N298A mutant of Synechocystis sp. PCC 6803 exhibits an O2 evolution activity of ∼10% of the wild-type. D1-N298A and the wild-type D1 had very similar features of thermoluminescence glow curves and of an FTIR difference spectrum upon YZ oxidation, suggesting that the hydrogen-bonded structure of YZ and electron transfer from the Mn4CaO5 cluster to YZ were little affected by substitution. In the D1-N298A mutant, however, the flash-number dependence of delayed luminescence showed a monotonic increase without oscillation, and FTIR difference spectra of the S-state cycle indicated partial and significant inhibition of the S2 → S3 and S3 → S0 transitions, respectively. These results suggest that the D1-N298A substitution inhibits the proton transfer processes in the S2 → S3 and S3 → S0 transitions. This in turn indicates that the hydrogen-bond network near YZ can be functional as a proton transfer pathway during photosynthetic water oxidation. ", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "ファイル公開日: 2018/12/08 ", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Biochemistry and Molecular Biology", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1074/jbc.M117.815183", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This research was originally published in the Journal of Biological Chemistry. Ryo Nagao, Hanayo Ueoka-Nakanishi, and Takumi Noguchi. D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation. J. Biol. Chem. 2017; 292:200046-20057. © the American Society for Biochemistry and Molecular Biology or © the Author(s).", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nagao, Ryo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76141", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueoka-Nakanishi, Hanayo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76142", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Noguchi, Takumi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76143", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-12-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "J_BiolChem-2017-Nagao-20046-57.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "J_BiolChem-2017-Nagao-20046-57", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/25707/files/J_BiolChem-2017-Nagao-20046-57.pdf"}, "version_id": "cd861f06-6e8e-4bcc-b5c7-7f121ca5541c"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cyanobacteria", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fourier transform IR (FTIR)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "photosynthesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "photosystem II", "subitem_subject_scheme": "Other"}, {"subitem_subject": "site-directed mutagenesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "D1-Asn298", "subitem_subject_scheme": "Other"}, {"subitem_subject": "YZ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proton trasfer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "thermoluminescence", "subitem_subject_scheme": "Other"}, {"subitem_subject": "water oxidation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["696"], "permalink_uri": "http://hdl.handle.net/2237/00027915", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-04-17"}, "publish_date": "2018-04-17", "publish_status": "0", "recid": "25707", "relation": {}, "relation_version_is_last": true, "title": ["D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation"], "weko_shared_id": -1}