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  1. D400 創薬科学研究科
  2. D400a 雑誌掲載論文
  3. 学術雑誌

Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant

http://hdl.handle.net/2237/00027927
862c8000-20b9-44fe-96ba-7216dac81b4c
名前 / ファイル ライセンス アクション
Hitomi_FEBS_J_2017.pdf Hitomi_FEBS_J_2017 (2.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-04-20
タイトル
タイトル Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant
著者 Horimizu, Rima

× Horimizu, Rima

WEKO 76234

Horimizu, Rima

Search repository
Ogawa, Ryota

× Ogawa, Ryota

WEKO 76235

Ogawa, Ryota

Search repository
Watanabe, Yuko

× Watanabe, Yuko

WEKO 76236

Watanabe, Yuko

Search repository
Tatsukawa, Hideki

× Tatsukawa, Hideki

WEKO 76237

Tatsukawa, Hideki

Search repository
Kinoshita, Masato

× Kinoshita, Masato

WEKO 76238

Kinoshita, Masato

Search repository
Hashimoto, Hisashi

× Hashimoto, Hisashi

WEKO 76239

Hashimoto, Hisashi

Search repository
Hitomi, Kiyotaka

× Hitomi, Kiyotaka

WEKO 76240

Hitomi, Kiyotaka

Search repository
権利
権利情報 "This is the peer reviewed version of the following article: [Horimizu, R. , Ogawa, R. , Watanabe, Y. , Tatsukawa, H. , Kinoshita, M. , Hashimoto, H. and Hitomi, K. (2017), Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene‐edited mutant. FEBS J, 284: 2843-2855. doi:10.1111/febs.14153], which has been published in final form at [https://doi.org/10.1111/febs.14153]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
キーワード
主題Scheme Other
主題 medaka
キーワード
主題Scheme Other
主題 transglutaminase
キーワード
主題Scheme Other
主題 gene-editing
キーワード
主題Scheme Other
主題 coagulation
抄録
内容記述 In the final process of blood coagulation, fibrin molecules are stabilized via a catalytic reaction by Factor XIIIA (FXIIIA), a member of the transglutaminase (TGase) family that catalyzes protein cross‐linking reactions. In this study, we characterized the orthologue of this enzyme in medaka (Oryzias latipes), an established model fish in which a coagulation system is also preserved. The recombinant protein of this orthologue enzyme was produced in baculovirus‐infected insect cells and used for analysis of its biochemical properties including activation by thrombin proteolysis and calcium dependence of the TGase enzymatic activity. Immunostaining and immunoblotting revealed that medaka FXIIIA is expressed in the kidney, bone, and esophagus in addition to blood cells. Furthermore, a gene‐mutant fish was established using the CRISPR/Cas9 system. The loss of FXIIIA expression was validated in the mutants, and phenotypes, such as absence of fibrin cross‐linking, were investigated in the established mutant fish.
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開日: 2018/09/01
内容記述タイプ Other
出版者
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1111/febs.14153
ISSN
収録物識別子タイプ ISSN
収録物識別子 1742464X
書誌情報 The FEBS Journal

巻 284, 号 17, p. 2843-2855, 発行日 2017-09
著者版フラグ
値 author
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