{"_buckets": {"deposit": "5d4376c9-9811-4802-a066-97ede796fe7c"}, "_deposit": {"id": "25719", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25719"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025719", "sets": ["1905"]}, "author_link": ["76234", "76235", "76236", "76237", "76238", "76239", "76240"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "17", "bibliographicPageEnd": "2855", "bibliographicPageStart": "2843", "bibliographicVolumeNumber": "284", "bibliographic_titles": [{"bibliographic_title": "The FEBS Journal", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In the final process of blood coagulation, fibrin molecules are stabilized via a catalytic reaction by Factor XIIIA (FXIIIA), a member of the transglutaminase (TGase) family that catalyzes protein cross‐linking reactions. In this study, we characterized the orthologue of this enzyme in medaka (Oryzias latipes), an established model fish in which a coagulation system is also preserved. The recombinant protein of this orthologue enzyme was produced in baculovirus‐infected insect cells and used for analysis of its biochemical properties including activation by thrombin proteolysis and calcium dependence of the TGase enzymatic activity. Immunostaining and immunoblotting revealed that medaka FXIIIA is expressed in the kidney, bone, and esophagus in addition to blood cells. Furthermore, a gene‐mutant fish was established using the CRISPR/Cas9 system. The loss of FXIIIA expression was validated in the mutants, and phenotypes, such as absence of fibrin cross‐linking, were investigated in the established mutant fish.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "ファイル公開日: 2018/09/01", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Wiley", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1111/febs.14153", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is the peer reviewed version of the following article: [Horimizu, R. , Ogawa, R. , Watanabe, Y. , Tatsukawa, H. , Kinoshita, M. , Hashimoto, H. and Hitomi, K. (2017), Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene‐edited mutant. FEBS J, 284: 2843-2855. doi:10.1111/febs.14153], which has been published in final form at [https://doi.org/10.1111/febs.14153]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1742464X", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Horimizu, Rima", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76234", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogawa, Ryota", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76235", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Yuko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76236", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tatsukawa, Hideki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76237", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kinoshita, Masato", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76238", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hashimoto, Hisashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76239", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hitomi, Kiyotaka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "76240", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-09-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Hitomi_FEBS_J_2017.pdf", "filesize": [{"value": "2.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2300000.0, "url": {"label": "Hitomi_FEBS_J_2017", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/25719/files/Hitomi_FEBS_J_2017.pdf"}, "version_id": "c4dbd1f3-3c80-4215-8030-2b5f902ce2eb"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "medaka", "subitem_subject_scheme": "Other"}, {"subitem_subject": "transglutaminase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gene-editing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "coagulation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["1905"], "permalink_uri": "http://hdl.handle.net/2237/00027927", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-04-20"}, "publish_date": "2018-04-20", "publish_status": "0", "recid": "25719", "relation": {}, "relation_version_is_last": true, "title": ["Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant"], "weko_shared_id": -1}