{"_buckets": {"deposit": "5d4376c9-9811-4802-a066-97ede796fe7c"}, "_deposit": {"id": "25719", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25719"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025719"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "17", "bibliographicPageEnd": "2855", "bibliographicPageStart": "2843", "bibliographicVolumeNumber": "284", "bibliographic_titles": [{"bibliographic_title": "The FEBS Journal"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "In the final process of blood coagulation, fibrin molecules are stabilized via a catalytic reaction by Factor XIIIA (FXIIIA), a member of the transglutaminase (TGase) family that catalyzes protein cross\u2010linking reactions. In this study, we characterized the orthologue of this enzyme in medaka (Oryzias latipes), an established model fish in which a coagulation system is also preserved. The recombinant protein of this orthologue enzyme was produced in baculovirus\u2010infected insect cells and used for analysis of its biochemical properties including activation by thrombin proteolysis and calcium dependence of the TGase enzymatic activity. Immunostaining and immunoblotting revealed that medaka FXIIIA is expressed in the kidney, bone, and esophagus in addition to blood cells. Furthermore, a gene\u2010mutant fish was established using the CRISPR/Cas9 system. The loss of FXIIIA expression was validated in the mutants, and phenotypes, such as absence of fibrin cross\u2010linking, were investigated in the established mutant fish.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5: 2018/09/01", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Wiley"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1111/febs.14153", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\"This is the peer reviewed version of the following article: [Horimizu, R. , Ogawa, R. , Watanabe, Y. , Tatsukawa, H. , Kinoshita, M. , Hashimoto, H. and Hitomi, K. (2017), Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene\u2010edited mutant. FEBS J, 284: 2843-2855. doi:10.1111/febs.14153], which has been published in final form at [https://doi.org/10.1111/febs.14153]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.\""}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1742464X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Horimizu, Rima"}], "nameIdentifiers": [{"nameIdentifier": "76234", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogawa, Ryota"}], "nameIdentifiers": [{"nameIdentifier": "76235", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Yuko"}], "nameIdentifiers": [{"nameIdentifier": "76236", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tatsukawa, Hideki"}], "nameIdentifiers": [{"nameIdentifier": "76237", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kinoshita, Masato"}], "nameIdentifiers": [{"nameIdentifier": "76238", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hashimoto, Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "76239", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hitomi, Kiyotaka"}], "nameIdentifiers": [{"nameIdentifier": "76240", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-09-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Hitomi_FEBS_J_2017.pdf", "filesize": [{"value": "2.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2300000.0, "url": {"label": "Hitomi_FEBS_J_2017", "url": "https://nagoya.repo.nii.ac.jp/record/25719/files/Hitomi_FEBS_J_2017.pdf"}, "version_id": "c4dbd1f3-3c80-4215-8030-2b5f902ce2eb"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "medaka", "subitem_subject_scheme": "Other"}, {"subitem_subject": "transglutaminase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gene-editing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "coagulation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant"}]}, "item_type_id": "10", "owner": "1", "path": ["1903/1904/1905"], "permalink_uri": "http://hdl.handle.net/2237/00027927", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2018-04-20"}, "publish_date": "2018-04-20", "publish_status": "0", "recid": "25719", "relation": {}, "relation_version_is_last": true, "title": ["Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant"], "weko_shared_id": null}