ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "7ae66390-037b-4903-ac1d-fd33ae28e926"}, "_deposit": {"id": "26012", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "26012"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00026012", "sets": ["2091"]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "153", "bibliographicPageStart": "141", "bibliographicVolumeNumber": "80", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Recently, the effects of stem cell supernatants or exosomes, such as skin wounds, have attracted at- tention. However, the effects of the induced pluripotent stem (iPS) cell-derived exosomes (iPS-Exos) have not been investigated in detail. Here, we investigated the effects of iPS-Exos on skin wound healing using an animal model. We isolated iPS-Exos from the iPS cell culture media. Control exosomes were isolated from unused iPS cell culture media (M-Exos). We first observed the morphologic characteristics of the isolated exosomes and examined the expression of surface antigens. The effects of these exosomes on the migratory response and proliferation of fibroblasts were analyzed as well. Additionally, using a diabetic ulcer model, the effects of iPS-Exos and M-Exos on skin wound healing were investigated. Transmission electron microscope analysis demonstrated that the size of iPS-Exos (120 ± 25 nm) was significantly larger than that of M-Exos (≤ 100 nm). Flow cytometry analyses showed that iPS-Exos were positive for CD9, CD63, and CD81, whereas they were negative for HLA-ABC and -DR expression. The migratory ability of fibroblasts cocultured with iPS-Exos was shown to be higher than that of the cells cocultured with M-Exos, as demonstrated using scratch assay. Skin wound healing model results showed that the administration of iPS-Exos results in a faster wound closure compared with that observed in the M-Exo group. In conclusion, the results obtained here indicate that iPS-Exos may promote the migration of fibroblasts in vitro and in vivo, suggesting the possibility of using iPS-Exos for the treatment of diabetic ulcer. ", "subitem_description_type": "Abstract"}]}, "item_9_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "This research was supported by the Challenging Sprouts Research Program through the Japan Society for the Promotion of Science (7115K15652).", "subitem_description_type": "Other"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.80.2.141", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University Graduate School of Medicine, School of Medicine"}]}, "item_9_relation_43": {"attribute_name": "異版である", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/802.html ", "subitem_relation_type_select": "URI"}}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN(print)", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kobayashi, Hitoshi"}], "nameIdentifiers": [{"nameIdentifier": "77370", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ebisawa, Katsumi"}], "nameIdentifiers": [{"nameIdentifier": "77371", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kambe, Miki"}], "nameIdentifiers": [{"nameIdentifier": "77372", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kasai, Takatoshi"}], "nameIdentifiers": [{"nameIdentifier": "77373", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suga, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "77374", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura, Kae"}], "nameIdentifiers": [{"nameIdentifier": "77375", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Narita, Yuji"}], "nameIdentifiers": [{"nameIdentifier": "77376", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogata, Aika"}], "nameIdentifiers": [{"nameIdentifier": "77377", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamei, Yuzuru"}], "nameIdentifiers": [{"nameIdentifier": "77378", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-05-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "01_Kobayashi_H.pdf", "filesize": [{"value": "12.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 12800000.0, "url": {"label": "01_Kobayashi_H.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/26012/files/01_Kobayashi_H.pdf"}, "version_id": "e3629c4b-f159-4a86-a4bf-2f6a984df2ff"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "exosome", "subitem_subject_scheme": "Other"}, {"subitem_subject": "induced pluripotent stem cell", "subitem_subject_scheme": "Other"}, {"subitem_subject": "wound healing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "diabetic ulcer", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effects of exosomes derived from the induced pluripotent stem cells on skin wound healing", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effects of exosomes derived from the induced pluripotent stem cells on skin wound healing"}]}, "item_type_id": "9", "owner": "1", "path": ["2091"], "permalink_uri": "https://doi.org/10.18999/nagjms.80.2.141", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-05-28"}, "publish_date": "2018-05-28", "publish_status": "0", "recid": "26012", "relation": {}, "relation_version_is_last": true, "title": ["Effects of exosomes derived from the induced pluripotent stem cells on skin wound healing"], "weko_shared_id": 3}
  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 80(2)

Effects of exosomes derived from the induced pluripotent stem cells on skin wound healing

https://doi.org/10.18999/nagjms.80.2.141
https://doi.org/10.18999/nagjms.80.2.141
4c23c9f5-4367-43e5-a5eb-c94a7ef94c2a
名前 / ファイル ライセンス アクション
01_Kobayashi_H.pdf 01_Kobayashi_H.pdf (12.8 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2018-05-28
タイトル
タイトル Effects of exosomes derived from the induced pluripotent stem cells on skin wound healing
著者 Kobayashi, Hitoshi

× Kobayashi, Hitoshi

WEKO 77370

Kobayashi, Hitoshi

Search repository
Ebisawa, Katsumi

× Ebisawa, Katsumi

WEKO 77371

Ebisawa, Katsumi

Search repository
Kambe, Miki

× Kambe, Miki

WEKO 77372

Kambe, Miki

Search repository
Kasai, Takatoshi

× Kasai, Takatoshi

WEKO 77373

Kasai, Takatoshi

Search repository
Suga, Hidetaka

× Suga, Hidetaka

WEKO 77374

Suga, Hidetaka

Search repository
Nakamura, Kae

× Nakamura, Kae

WEKO 77375

Nakamura, Kae

Search repository
Narita, Yuji

× Narita, Yuji

WEKO 77376

Narita, Yuji

Search repository
Ogata, Aika

× Ogata, Aika

WEKO 77377

Ogata, Aika

Search repository
Kamei, Yuzuru

× Kamei, Yuzuru

WEKO 77378

Kamei, Yuzuru

Search repository
キーワード
主題Scheme Other
主題 exosome
キーワード
主題Scheme Other
主題 induced pluripotent stem cell
キーワード
主題Scheme Other
主題 wound healing
キーワード
主題Scheme Other
主題 diabetic ulcer
抄録
内容記述 Recently, the effects of stem cell supernatants or exosomes, such as skin wounds, have attracted at- tention. However, the effects of the induced pluripotent stem (iPS) cell-derived exosomes (iPS-Exos) have not been investigated in detail. Here, we investigated the effects of iPS-Exos on skin wound healing using an animal model. We isolated iPS-Exos from the iPS cell culture media. Control exosomes were isolated from unused iPS cell culture media (M-Exos). We first observed the morphologic characteristics of the isolated exosomes and examined the expression of surface antigens. The effects of these exosomes on the migratory response and proliferation of fibroblasts were analyzed as well. Additionally, using a diabetic ulcer model, the effects of iPS-Exos and M-Exos on skin wound healing were investigated. Transmission electron microscope analysis demonstrated that the size of iPS-Exos (120 ± 25 nm) was significantly larger than that of M-Exos (≤ 100 nm). Flow cytometry analyses showed that iPS-Exos were positive for CD9, CD63, and CD81, whereas they were negative for HLA-ABC and -DR expression. The migratory ability of fibroblasts cocultured with iPS-Exos was shown to be higher than that of the cells cocultured with M-Exos, as demonstrated using scratch assay. Skin wound healing model results showed that the administration of iPS-Exos results in a faster wound closure compared with that observed in the M-Exo group. In conclusion, the results obtained here indicate that iPS-Exos may promote the migration of fibroblasts in vitro and in vivo, suggesting the possibility of using iPS-Exos for the treatment of diabetic ulcer.
内容記述タイプ Abstract
内容記述
内容記述 This research was supported by the Challenging Sprouts Research Program through the Japan Society for the Promotion of Science (7115K15652).
内容記述タイプ Other
出版者
出版者 Nagoya University Graduate School of Medicine, School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.80.2.141
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/802.html
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
書誌情報 Nagoya Journal of Medical Science

巻 80, 号 2, p. 141-153, 発行日 2018-05
著者版フラグ
値 publisher
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 12:49:47.713320
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3