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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Involvement of laccase2 and yellow-e genes in antifungal host defense of the model beetle, Tribolium castaneum

http://hdl.handle.net/2237/00028293
0995cbf7-d471-4849-950c-05d611506ddb
名前 / ファイル ライセンス アクション
JIP_2017_243_Revision JIP_2017_243_Revision 1_V0 (1.7 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-06-11
タイトル
タイトル Involvement of laccase2 and yellow-e genes in antifungal host defense of the model beetle, Tribolium castaneum
著者 Hayakawa, Yuuki

× Hayakawa, Yuuki

WEKO 78155

Hayakawa, Yuuki

Search repository
Sawada, Mitsuki

× Sawada, Mitsuki

WEKO 78156

Sawada, Mitsuki

Search repository
Seki, Masaki

× Seki, Masaki

WEKO 78157

Seki, Masaki

Search repository
Sirasoonthorn, Patchara

× Sirasoonthorn, Patchara

WEKO 78158

Sirasoonthorn, Patchara

Search repository
Shiga, Shotaro

× Shiga, Shotaro

WEKO 78159

Shiga, Shotaro

Search repository
Kamiya, Katsumi

× Kamiya, Katsumi

WEKO 78160

Kamiya, Katsumi

Search repository
Minakuchi, Chieka

× Minakuchi, Chieka

WEKO 78161

Minakuchi, Chieka

Search repository
Miura, Ken

× Miura, Ken

WEKO 78162

Miura, Ken

Search repository
権利
権利情報 © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
抄録
内容記述 We previously reported that the moderate knockdown of chitin synthase 1 gene of the model beetle Tribolium castaneum impairs the host defense against entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, which infect host insects via the direct penetration of cuticular integuments (Hayakawa et al., 2017). In this study, we focused on the antifungal roles of laccase2 (Lac2) as well as yellow-e (Y-e) genes, both of which are shown to be important to the establishment of stable cuticular structures in this beetle species. The expression profiles of the two genes somewhat resembled each other, peaking in late prepupae and mid to late pupae, while the transcript levels of Lac2 were higher than Y-e throughout. The knockdown of Lac2 gene at the prepupal and pupal peaks with relatively small amounts of dsRNA resulted in pupae with a lighter color and adults with a lighter color and dimpled/wrinkled elytra, respectively. Meanwhile, similar gene knockdown of Y-e but with 10 times more dsRNA compared to Lac2 resulted in pupae having a normal appearance and adults with a darker color. We conducted fungal infection assays with B. bassiana and M. anisopliae using these knockdown animals. The knockdown of Y-e gene had no or limited effects in both pupae and adults in terms of the antifungal host defense. Similarly, the knockdown of Lac2 gene did not change significantly the defense phenotypes of the resulting pupae. By sharp contrast, the host defense of the adult beetles against the two fungal species was almost totally destroyed by the moderate knockdown of Lac2 gene, suggesting its indispensable role in antifungal host defense presumably through the construction of sound cuticles of the adults. Finally, we investigated the maturation of host defense against fungal infection in the Lac2 knockdown adults and found that while the day 10 adults were still susceptible to M. anisopliae infection with some delay of death in comparison with day 1 adults, they exhibited complete refractoriness to B. bassiana.
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2019-01-01
内容記述タイプ Other
出版者
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jip.2017.10.010
ISSN
収録物識別子タイプ ISSN
収録物識別子 0022-2011
書誌情報 Journal of Invertebrate Pathology

巻 151, p. 41-49, 発行日 2018-01
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