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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Development of a method to preliminarily embed tissue samples using low melting temperature fish gelatin before sectioning: A technical note

http://hdl.handle.net/2237/00028335
facd2a6b-7d69-4a80-acd3-a8af8b1c045c
名前 / ファイル ライセンス アクション
Ushida_et_al.pdf Ushida_et_al (3.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-07-04
タイトル
タイトル Development of a method to preliminarily embed tissue samples using low melting temperature fish gelatin before sectioning: A technical note
著者 Ushida, Kaori

× Ushida, Kaori

WEKO 78385

Ushida, Kaori

Search repository
Asai, Naoya

× Asai, Naoya

WEKO 78386

Asai, Naoya

Search repository
Uchiyama, Kozo

× Uchiyama, Kozo

WEKO 78387

Uchiyama, Kozo

Search repository
Enomoto, Atsushi

× Enomoto, Atsushi

WEKO 78388

Enomoto, Atsushi

Search repository
Takahashi, Masahide

× Takahashi, Masahide

WEKO 78389

Takahashi, Masahide

Search repository
権利
権利情報 "This is the peer reviewed version of the following article: [Ushida, K. , Asai, N. , Uchiyama, K. , Enomoto, A. and Takahashi, M. (2018), Development of a method to preliminarily embed tissue samples using low melting temperature fish gelatin before sectioning: A technical note. Pathol Int, 68: 241-245. doi:10.1111/pin.12652], which has been published in final form at [http://doi.org/10.1111/pin.12652]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
キーワード
主題Scheme Other
主題 low melting temperature gelatin
キーワード
主題Scheme Other
主題 paraffin embedding
キーワード
主題Scheme Other
主題 preliminary embedding
キーワード
主題Scheme Other
主題 tissue sample preparation
抄録
内容記述 Embedding of tissue samples that maintains a desired orientation is critical for preparing sections suitable for diagnosis and study objectives. Methods to prepare tissue sections include: (i) paraffin embedding or snap‐freezing followed by microtome or cryostat sectioning; and (ii) agarose embedding followed by cutting on a vibrating microslicer. Although these methods are useful for routine laboratory work, preparation of small and fragile tissues such as mouse organs, small human biopsy samples, and cultured floating spheres is difficult and requires special skills. In particular, tissue specimen orientation can be lost during embedding in molds and subsequent sectioning. Here, we developed a method using low melting temperature (LM) gelatin either alone or mixed with agarose to preliminarily embed collected tissues that are either prefixed or unfixed, followed by conventional fixation, paraffin embedding, freezing, and sectioning. The advantage of the method is that the LM gelatin and its mixture with agarose can be handled at room temperature but quickly hardens at 4°C, which allows embedding, trimming, and arranging of small and fragile tissues in a desired orientation and are compatible with traditional stainings. Thus, this method can have various laboratory applications and can be modified according to the needs of each laboratory.
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2019-04-02
内容記述タイプ Other
出版者
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1111/pin.12652
ISSN
収録物識別子タイプ ISSN
収録物識別子 13205463
書誌情報 Pathology International

巻 68, 号 4, p. 241-245, 発行日 2018
著者版フラグ
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