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Apical-to-basolateral transepithelial transport of cow’s milk caseins by intestinal Caco-2 cell monolayers: MS-based quantitation of cellularly degraded α- and β-casein fragments
http://hdl.handle.net/2237/00028382
http://hdl.handle.net/2237/0002838263a7938a-524c-4cd4-8209-c479b582c544
名前 / ファイル | ライセンス | アクション |
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J_Biochem_Sakurai_et_al_TEXT (330.1 kB)
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Sakurai_et_al_Figs (7.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-07-13 | |||||
タイトル | ||||||
タイトル | Apical-to-basolateral transepithelial transport of cow’s milk caseins by intestinal Caco-2 cell monolayers: MS-based quantitation of cellularly degraded α- and β-casein fragments | |||||
言語 | en | |||||
著者 |
Sakurai, Nao
× Sakurai, Nao× Nishio, Shunsuke× Akiyama, Yuka× Miyata, Shinji× Oshima, Kenzi× Nadano, Daita× Matsuda, Tsukasa |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is a pre-copyedited, author-produced version of an article accepted for publication in [Japanese Journal of Clinical Oncology] following peer review. The version of record [Nao Sakurai, Shunsuke Nishio, Yuka Akiyama, Shinji Miyata, Kenzi Oshima, Daita Nadano, Tsukasa Matsuda; Apical-to-basolateral transepithelial transport of cow’s milk caseins by intestinal Caco-2 cell monolayers: MS-based quantitation of cellularly degraded α- and β-casein fragments, The Journal of Biochemistry, Volume 164, Issue 2, 1 August 2018, Pages 113–125, https://doi.org/10.1093/jb/mvy034] is available online at: https://doi.org/10.1093/jb/mvy034. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antigen processing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | degradation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lysosomes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mass spectroscopy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polarity | |||||
抄録 | ||||||
内容記述 | Casein (CN) is the major milk protein to nourish infants but, in certain population, it causes cow’s milk allergy, indicating the uptake of antigenic CN and their peptides through the intestinal epithelium. Using human intestinal Caco-2 cell monolayers, the apical-to-basal transepithelial transport of CN was investigated. Confocal microscopy using component-specific antibodies showed that αs1-CN antigens became detectable as punctate signals at the apical-side cytoplasm and reached to the cytoplasm at a tight-junction level within a few hours. Such intracellular CN signals were more remarkable than those of the other antigens, β-lactoglobulin and ovalbumin, colocalized in part with an early endosome marker protein (EEA1) and decreased in the presence of cytochalasin D or sodium azide and also at lowered temperature at 4°C. Liquid chromatography coupled with mass spectroscopy analysis of the protein fraction in the basal-side medium identified the αs1-CB fragment including the N-terminal region and the αs2-CN fragment containing the central part of polypeptide at 100–1,000 fmol per well levels. Moreover, β-CN C-terminal overlapping peptides were identified in the peptide fraction below 10 kDa of the basal medium. These results suggest that CNs are partially degraded by cellular proteases and/or peptidases and immunologically active CN fragments are transported to basal side of the cell monolayers. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | ファイル公開日: 2019/08/01 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Oxford University Press | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1093/jb/mvy034 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0021-924X | |||||
書誌情報 |
en : The Journal of Biochemistry 巻 164, 号 2, p. 113-125, 発行日 2018-08 |
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著者版フラグ | ||||||
値 | author |