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Vwf K1362A resulted in failure of protein synthesis in mice
http://hdl.handle.net/2237/00028521
http://hdl.handle.net/2237/00028521c49b9d4e-14c8-4da2-9715-b9581b272b23
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
IJHM-D-17-00732_R1 (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-08-24 | |||||
| タイトル | ||||||
| タイトル | Vwf K1362A resulted in failure of protein synthesis in mice | |||||
| 言語 | en | |||||
| 著者 |
Sanda, Naomi
× Sanda, Naomi× Suzuki, Nobuaki× Suzuki, Atsuo× Kanematsu, Takeshi× Kishimoto, Mayuko× Hasuwa, Hidetoshi× Takagi, Akira× Kojima, Tetsuhito× Matsushita, Tadashi× Nakamura, Shigeo |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | “This is a post-peer-review, pre-copyedit version of an article published in [International Journal of Hematology]. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12185-017-2394-y”. | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Von Willebrand factor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Mouse model | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Genetic mutation | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Von Willebrand factor (VWF) is synthesized in megakaryocytes and endothelial cells (ECs) and has two main roles: to carry and protect coagulation factor VIII (FVIII) from degradation by forming VWF–FVIII complex; and to mediate platelet adhesion and aggregation at sites of vascular injury. Previous research using the HEK293 cell line revealed that the VWF K1362 mutation interacted directly with platelet glycoprotein Ib (GPIb). Vwf K1362A knock-in (KI) mice were therefore generated to verify the in vivo function of residue 1362 in binding to platelet GPIb. The Cre-loxP system was employed to introduce the Vwf K1362A mutation systemically in mice. In blood coagulation analysis, the VWF antigen (VWF:Ag) of Lys1362Ala KI homozygous (homo) mice was below the sensitivity of detection by enzyme-linked immunosorbent assay. FVIII activities (FVIII:C) were 47.9 ± 0.3 and 3.3 ± 0.3% (K1362A heterozygous (hetero) and K1362A KI homo mice, respectively) compared to wild-type mice. Immunohistochemical staining analysis revealed that VWF protein did not exist in ECs of K1362A KI homo mice. These results indicated that VWF protein synthesis of K1362A was impaired after transcription in mice. K1362 seems to represent a very important position not only for VWF function, but also for VWF synthesis in mice. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ファイル公開：2019/04/01 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Springer | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s12185-017-2394-y | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0925-5710 | |||||
| 書誌情報 |
en : International Journal of Hematology 巻 107, 号 4, p. 428-435, 発行日 2018-04 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
