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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Vwf K1362A resulted in failure of protein synthesis in mice

http://hdl.handle.net/2237/00028521
http://hdl.handle.net/2237/00028521
c49b9d4e-14c8-4da2-9715-b9581b272b23
名前 / ファイル ライセンス アクション
IJHM-D-17-00732_R1.pdf IJHM-D-17-00732_R1 (1.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-08-24
タイトル
タイトル Vwf K1362A resulted in failure of protein synthesis in mice
言語 en
著者 Sanda, Naomi

× Sanda, Naomi

WEKO 84880

en Sanda, Naomi

Search repository
Suzuki, Nobuaki

× Suzuki, Nobuaki

WEKO 84881

en Suzuki, Nobuaki

Search repository
Suzuki, Atsuo

× Suzuki, Atsuo

WEKO 84882

en Suzuki, Atsuo

Search repository
Kanematsu, Takeshi

× Kanematsu, Takeshi

WEKO 84883

en Kanematsu, Takeshi

Search repository
Kishimoto, Mayuko

× Kishimoto, Mayuko

WEKO 84884

en Kishimoto, Mayuko

Search repository
Hasuwa, Hidetoshi

× Hasuwa, Hidetoshi

WEKO 84885

en Hasuwa, Hidetoshi

Search repository
Takagi, Akira

× Takagi, Akira

WEKO 84886

en Takagi, Akira

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Kojima, Tetsuhito

× Kojima, Tetsuhito

WEKO 84887

en Kojima, Tetsuhito

Search repository
Matsushita, Tadashi

× Matsushita, Tadashi

WEKO 84888

en Matsushita, Tadashi

Search repository
Nakamura, Shigeo

× Nakamura, Shigeo

WEKO 84889

en Nakamura, Shigeo

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 “This is a post-peer-review, pre-copyedit version of an article published in [International Journal of Hematology]. The final authenticated version is available online at: http://dx.doi.org/10.1007/s12185-017-2394-y”.
キーワード
主題Scheme Other
主題 Von Willebrand factor
キーワード
主題Scheme Other
主題 Mouse model
キーワード
主題Scheme Other
主題 Genetic mutation
抄録
内容記述 Von Willebrand factor (VWF) is synthesized in megakaryocytes and endothelial cells (ECs) and has two main roles: to carry and protect coagulation factor VIII (FVIII) from degradation by forming VWF–FVIII complex; and to mediate platelet adhesion and aggregation at sites of vascular injury. Previous research using the HEK293 cell line revealed that the VWF K1362 mutation interacted directly with platelet glycoprotein Ib (GPIb). Vwf K1362A knock-in (KI) mice were therefore generated to verify the in vivo function of residue 1362 in binding to platelet GPIb. The Cre-loxP system was employed to introduce the Vwf K1362A mutation systemically in mice. In blood coagulation analysis, the VWF antigen (VWF:Ag) of Lys1362Ala KI homozygous (homo) mice was below the sensitivity of detection by enzyme-linked immunosorbent assay. FVIII activities (FVIII:C) were 47.9 ± 0.3 and 3.3 ± 0.3% (K1362A heterozygous (hetero) and K1362A KI homo mice, respectively) compared to wild-type mice. Immunohistochemical staining analysis revealed that VWF protein did not exist in ECs of K1362A KI homo mice. These results indicated that VWF protein synthesis of K1362A was impaired after transcription in mice. K1362 seems to represent a very important position not only for VWF function, but also for VWF synthesis in mice.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2019/04/01
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Springer
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s12185-017-2394-y
ISSN
収録物識別子タイプ PISSN
収録物識別子 0925-5710
書誌情報 en : International Journal of Hematology

巻 107, 号 4, p. 428-435, 発行日 2018-04
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