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Utilization of an intermediate of the methylerythritol phosphate pathway, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate, as the prenyl donor substrate for various prenyltransferases
http://hdl.handle.net/2237/00028546
http://hdl.handle.net/2237/000285465d279ba6-f8b5-4a61-bdf3-f8bc047e5986
名前 / ファイル | ライセンス | アクション |
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manuscriptforNUrepository (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-09-10 | |||||
タイトル | ||||||
タイトル | Utilization of an intermediate of the methylerythritol phosphate pathway, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate, as the prenyl donor substrate for various prenyltransferases | |||||
言語 | en | |||||
著者 |
Hayashi, Yoshifumi
× Hayashi, Yoshifumi× Ito, Tomokazu× Yoshimura, Tohru× Hemmi, Hisashi |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is an Accepted Manuscript of an article published by Taylor & Francis Group in Bioscience, Biotechnology, and Biochemistry on 01/12/2017, available online: http://www.tandfonline.com/10.1080/09168451.2017.1398064. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Isoprenoid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | prenyltransferase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lycopene elongase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | flavuxanthin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | carotenoid | |||||
抄録 | ||||||
内容記述 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate (HMBPP) is an intermediate of the methylerythritol phosphate pathway. Utilization of HMBPP by lycopene elongase from Corynebacterium glutamicum, which is a UbiA-family prenyltransferase responsible for C50 carotenoid biosynthesis, was investigated using an Escherichia coli strain that contained the exogenous mevalonate pathway as well as the carotenoid biosynthetic pathway. Inhibition of the endogenous methylerythritol phosphate pathway resulted in loss of the production of C50 carotenoid flavuxanthin, while C40 lycopene formation was retained. Overexpression of E. coli ispH gene, which encodes HMBPP reductase, also decreased the production of flavuxanthin in E. coli cells. These results indicate the preference of lycopene elongase for HMBPP instead of the previously proposed substrate, dimethylallyl diphosphate. Furthermore, several (all-E)-prenyl diphosphate synthases, which are classified in a distinct family of prenyltransferase, were demonstrated to accept HMBPP, which implies that the compound is more widely used as a prenyl donor substrate than was previously expected. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | Published online: 01 Dec 2017. ファイル公開:2018/12/01 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Taylor & Francis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1080/09168451.2017.1398064 | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0916-8451 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 1347-6947 | |||||
書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 82, 号 6, p. 993-1002, 発行日 2018 |
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著者版フラグ | ||||||
値 | author |