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Septin-dependent remodeling of cortical microtubule drives cell reshaping during epithelial wound healing
http://hdl.handle.net/2237/00029136
http://hdl.handle.net/2237/00029136cc25ebe1-0660-4eb8-b1df-2012f358fdea
名前 / ファイル | ライセンス | アクション |
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jcs212647 (9.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-12-25 | |||||
タイトル | ||||||
タイトル | Septin-dependent remodeling of cortical microtubule drives cell reshaping during epithelial wound healing | |||||
言語 | en | |||||
著者 |
Shindo, Asako
× Shindo, Asako× Audrey, Anastasia× Takagishi, Maki× Takahashi, Masahide× Wallingford, John B.× Kinoshita, Makoto |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2018. Published by The Company of Biologists Ltd | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytoskeleton | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Embryonic wound healing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Epidermis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Xenopus laevis | |||||
抄録 | ||||||
内容記述 | Wounds in embryos heal rapidly through contraction of the wound edges. Despite well-recognized significance of the actomyosin purse string for wound closure, roles for other cytoskeletal components are largely unknown. Here, we report that the septin cytoskeleton cooperates with actomyosin and microtubules to coordinate circumferential contraction of the wound margin and concentric elongation of wound-proximal cells in Xenopus laevis embryos. Microtubules reoriented radially, forming bundles along lateral cell cortices in elongating wound-proximal cells. Depletion of septin 7 (Sept7) slowed wound closure by attenuating the wound edge contraction and cell elongation. ROCK/Rho-kinase inhibitor-mediated suppression of actomyosin contractility enhanced the Sept7 phenotype, whereas the Sept7 depletion did not affect the accumulation of actomyosin at the wound edge. The cortical microtubule bundles were reduced in wound-proximal cells in Sept7 knockdown (Sept7-KD) embryos, but forced bundling of microtubules mediated by the microtubule-stabilizing protein Map7 did not rescue the Sept7-KD phenotype. Nocodazole-mediated microtubule depolymerization enhanced the Sept7-KD phenotype, suggesting that Sept7 is required for microtubule reorganization during cell elongation. Our findings indicate that septins are required for the rapid wound closure by facilitating cortical microtubule reorganization and the concentric elongation of surrounding cells. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | ファイル公開:2019-06-28 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | The Company of Biologists | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1242/jcs.212647 | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0021-9533 | |||||
書誌情報 |
en : Journal of Cell Science 巻 131, 号 12, p. jcs212647, 発行日 2018-11 |
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著者版フラグ | ||||||
値 | publisher |