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Partial Identification of Amyloid-β Degrading Activity in Human Serum
https://doi.org/10.18999/nagjms.81.1.55
https://doi.org/10.18999/nagjms.81.1.55ae27af70-1763-4fef-a796-3fa57abb95cb
名前 / ファイル | ライセンス | アクション |
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05_Mikawa.pdf (1.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-03-15 | |||||
タイトル | ||||||
タイトル | Partial Identification of Amyloid-β Degrading Activity in Human Serum | |||||
言語 | en | |||||
著者 |
Mikawa, Ryuta
× Mikawa, Ryuta× Okuno, Alato× Yoshimi, Tatsuya× Watanabe, Atsushi× Maruyama, Mitsuo× Takikawa, Osamu |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報Resource | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
権利情報 | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Alzheimer’s disease | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | amyloid-beta-degrading activity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human serum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | brain microvessel | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The major hallmarks of Alzheimer’s disease (AD) are the extracellular accumulation of pathological amyloid beta (Aβ) in the brain parenchyma and Aβ deposition in cerebral blood walls (cerebral amyloid angiopathy; CAA). Although CAA occurs in more than 80% of AD patients, the mechanisms of Aβ deposition and clearance around the vessel walls are unknown. We found Aβ-degrading activity in human serum during analysis of the regulatory mechanism of Aβ production in human endothelial cells. To elucidate the metabolic dynamics of Aβ surrounding the brain microvessels, we identified Aβ-degrading activity in human serum (blood Aβ-degrading activity: BADA) by column chromatography and LC/MS. BADA exhibited characteristics of an acidic protein, pI 4.3, which had two different protein surface charges (low and high affinity cations). Both BADA fractions had a relative molecular mass of greater than 400 kDa. Furthermore, BADA in the low affinity cation fraction was inhibited by the serine protease inhibitor 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF). We clarified alpha-2-macroglobulin (a2M) and several serine proteases from this BADA by LC-MS. Moreover, we demonstrated that BADA is increased by approximately 5000-fold in human serum by column chromatography. Therefore, BADA may play an important role in the circulation and metabolism of Aβ in human brain microvessels. | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | Nagoya University Graduate School of Medicine, School of Medicine | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.81.1.55 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/811.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 81, 号 1, p. 55-64, 発行日 2019-02 |
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著者版フラグ | ||||||
値 | publisher |