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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 81(1)

Partial Identification of Amyloid-β Degrading Activity in Human Serum

https://doi.org/10.18999/nagjms.81.1.55
https://doi.org/10.18999/nagjms.81.1.55
ae27af70-1763-4fef-a796-3fa57abb95cb
名前 / ファイル ライセンス アクション
05_Mikawa.pdf 05_Mikawa.pdf (1.1 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2019-03-15
タイトル
タイトル Partial Identification of Amyloid-β Degrading Activity in Human Serum
著者 Mikawa, Ryuta

× Mikawa, Ryuta

WEKO 89412

Mikawa, Ryuta

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Okuno, Alato

× Okuno, Alato

WEKO 89413

Okuno, Alato

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Yoshimi, Tatsuya

× Yoshimi, Tatsuya

WEKO 89414

Yoshimi, Tatsuya

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Watanabe, Atsushi

× Watanabe, Atsushi

WEKO 89415

Watanabe, Atsushi

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Maruyama, Mitsuo

× Maruyama, Mitsuo

WEKO 89416

Maruyama, Mitsuo

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Takikawa, Osamu

× Takikawa, Osamu

WEKO 89417

Takikawa, Osamu

Search repository
キーワード
主題Scheme Other
主題 Alzheimer’s disease
キーワード
主題Scheme Other
主題 amyloid-beta-degrading activity
キーワード
主題Scheme Other
主題 human serum
キーワード
主題Scheme Other
主題 brain microvessel
抄録
内容記述 The major hallmarks of Alzheimer’s disease (AD) are the extracellular accumulation of pathological amyloid beta (Aβ) in the brain parenchyma and Aβ deposition in cerebral blood walls (cerebral amyloid angiopathy; CAA). Although CAA occurs in more than 80% of AD patients, the mechanisms of Aβ deposition and clearance around the vessel walls are unknown. We found Aβ-degrading activity in human serum during analysis of the regulatory mechanism of Aβ production in human endothelial cells. To elucidate the metabolic dynamics of Aβ surrounding the brain microvessels, we identified Aβ-degrading activity in human serum (blood Aβ-degrading activity: BADA) by column chromatography and LC/MS. BADA exhibited characteristics of an acidic protein, pI 4.3, which had two different protein surface charges (low and high affinity cations). Both BADA fractions had a relative molecular mass of greater than 400 kDa. Furthermore, BADA in the low affinity cation fraction was inhibited by the serine protease inhibitor 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF). We clarified alpha-2-macroglobulin (a2M) and several serine proteases from this BADA by LC-MS. Moreover, we demonstrated that BADA is increased by approximately 5000-fold in human serum by column chromatography. Therefore, BADA may play an important role in the circulation and metabolism of Aβ in human brain microvessels.
内容記述タイプ Abstract
出版者
出版者 Nagoya University Graduate School of Medicine, School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.81.1.55
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/811.html
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 81, 号 1, p. 55-64, 発行日 2019-02
著者版フラグ
値 publisher
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