{"_buckets": {"deposit": "58a72830-b23c-494d-9805-c08cca6d915f"}, "_deposit": {"id": "27347", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "27347"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00027347"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "64", "bibliographicPageStart": "55", "bibliographicVolumeNumber": "81", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The major hallmarks of Alzheimer\u2019s disease (AD) are the extracellular accumulation of pathological amyloid beta (A\u03b2) in the brain parenchyma and A\u03b2 deposition in cerebral blood walls (cerebral amyloid angiopathy; CAA). Although CAA occurs in more than 80% of AD patients, the mechanisms of A\u03b2 deposition and clearance around the vessel walls are unknown. We found A\u03b2-degrading activity in human serum during analysis of the regulatory mechanism of A\u03b2 production in human endothelial cells. To elucidate the metabolic dynamics of A\u03b2 surrounding the brain microvessels, we identified A\u03b2-degrading activity in human serum (blood A\u03b2-degrading activity: BADA) by column chromatography and LC/MS. BADA exhibited characteristics of an acidic protein, pI 4.3, which had two different protein surface charges (low and high affinity cations). Both BADA fractions had a relative molecular mass of greater than 400 kDa. Furthermore, BADA in the low affinity cation fraction was inhibited by the serine protease inhibitor 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF). We clarified alpha-2-macroglobulin (a2M) and several serine proteases from this BADA by LC-MS. Moreover, we demonstrated that BADA is increased by approximately 5000-fold in human serum by column chromatography. Therefore, BADA may play an important role in the circulation and metabolism of A\u03b2 in human brain microvessels. ", "subitem_description_type": "Abstract"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.81.1.55", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University Graduate School of Medicine, School of Medicine"}]}, "item_9_relation_43": {"attribute_name": "\u7570\u7248\u3067\u3042\u308b", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/811.html ", "subitem_relation_type_select": "URI"}}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_61": {"attribute_name": "ISSN\uff08Online\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "2186-3326", "subitem_source_identifier_type": "ISSN"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mikawa, Ryuta"}], "nameIdentifiers": [{"nameIdentifier": "89412", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okuno, Alato"}], "nameIdentifiers": [{"nameIdentifier": "89413", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshimi, Tatsuya"}], "nameIdentifiers": [{"nameIdentifier": "89414", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "89415", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maruyama, Mitsuo"}], "nameIdentifiers": [{"nameIdentifier": "89416", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takikawa, Osamu"}], "nameIdentifiers": [{"nameIdentifier": "89417", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-03-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "05_Mikawa.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "05_Mikawa.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/27347/files/05_Mikawa.pdf"}, "version_id": "aac0f2fb-4cfa-4f92-9472-13ede70dd4a3"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Alzheimer\u2019s disease", "subitem_subject_scheme": "Other"}, {"subitem_subject": "amyloid-beta-degrading activity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "human serum", "subitem_subject_scheme": "Other"}, {"subitem_subject": "brain microvessel", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Partial Identification of Amyloid-\u03b2 Degrading Activity in Human Serum", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Partial Identification of Amyloid-\u03b2 Degrading Activity in Human Serum"}]}, "item_type_id": "9", "owner": "1", "path": ["499/508/509/2359"], "permalink_uri": "https://doi.org/10.18999/nagjms.81.1.55", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2019-03-15"}, "publish_date": "2019-03-15", "publish_status": "0", "recid": "27347", "relation": {}, "relation_version_is_last": true, "title": ["Partial Identification of Amyloid-\u03b2 Degrading Activity in Human Serum"], "weko_shared_id": 3}