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  1. D400 創薬科学研究科
  2. D400a 雑誌掲載論文
  3. 学術雑誌

Morphology-Based Analysis of Myoblasts for Prediction of Myotube Formation

http://hdl.handle.net/2237/00030226
http://hdl.handle.net/2237/00030226
94d83c3a-83d9-4664-953c-c044d7f4e083
名前 / ファイル ライセンス アクション
SLAS_finalmanuscript.pdf SLAS_finalmanuscript (66.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2019-05-15
タイトル
タイトル Morphology-Based Analysis of Myoblasts for Prediction of Myotube Formation
言語 en
著者 Ishikawa, Kiyoshi

× Ishikawa, Kiyoshi

WEKO 91280

en Ishikawa, Kiyoshi

Search repository
Yoshida, Kei

× Yoshida, Kei

WEKO 91281

en Yoshida, Kei

Search repository
Kanie, Kei

× Kanie, Kei

WEKO 91282

en Kanie, Kei

Search repository
Omori, Kenji

× Omori, Kenji

WEKO 91283

en Omori, Kenji

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Kato, Ryuji

× Kato, Ryuji

WEKO 91284

en Kato, Ryuji

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
権利情報 Ishikawa, K., Yoshida, K., Kanie, K., Omori, K., & Kato, R. (2019). Morphology-Based Analysis of Myoblasts for Prediction of Myotube Formation. SLAS DISCOVERY: Advancing Life Sciences R&D, 24(1), 47–56. https://doi.org/10.1177/2472555218793374. Copyright © [2019] (The Author(s)). DOI: [10.1177/2472555218793374].
キーワード
主題Scheme Other
主題 cellular morphology
キーワード
主題Scheme Other
主題 morphology-based analysis
キーワード
主題Scheme Other
主題 myoblast differentiation
キーワード
主題Scheme Other
主題 cGMP
抄録
内容記述 The development of new drugs depends on the efficiency of drug screening. Phenotype-based screening has attracted interest due to its considerable potency for the discovery of first-in-class drugs. In general, fluorescently labeled imagery is the leading technique for phenotype-based screening; however, there are growing requirements to understand total culture profiles, which are unclear after end-point assays. In this study, we demonstrate that morphology-based cellular evaluation of unlabeled cells is an efficient approach to evaluate myotube formation assays. One of our aims was to study the myogenic differentiation process in C2C12 cells to discern the differences between cellular responses to different medium conditions (serum concentrations and insulin dosages). Our results show that predictive morphological profiles that strongly correlate with myogenic differentiation can be generated from myotube images, even in the confluent stage. The differentiation rate after 14 days can be quantitatively predicted with the highest accuracy by means of images taken on days 0–11.5. In addition, for the application of our morphology-based cellular evaluation, the effect of cyclic guanosine monophosphate (cGMP) on myogenic differentiation was analyzed. Our results show that the quantitated morphological profile from these images can be an effective descriptor for analysis of the myotube-recovering effect of cGMP.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 SAGE Publications
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1177/2472555218793374
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 2472-5552
書誌情報 en : SLAS DISCOVERY: Advancing Life Sciences R&D

巻 24, 号 1, p. 47-56, 発行日 2019-01-01
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