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  1. H110 遺伝子実験施設
  2. H110a 雑誌掲載論文
  3. 学術雑誌

An evolutionarily conserved P-subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana

http://hdl.handle.net/2237/00030545
http://hdl.handle.net/2237/00030545
6b47b352-2df9-4f17-a83b-bcc90c297ae5
名前 / ファイル ライセンス アクション
TPJ-01005-2017.R1.pdf TPJ-01005-2017.R1 (3.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2019-07-11
タイトル
タイトル An evolutionarily conserved P-subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana
言語 en
著者 Ito, Ayaka

× Ito, Ayaka

WEKO 92620

en Ito, Ayaka

Search repository
Sugita, Chieko

× Sugita, Chieko

WEKO 92621

en Sugita, Chieko

Search repository
Ichinose, Mizuho

× Ichinose, Mizuho

WEKO 92622

en Ichinose, Mizuho

Search repository
Kato, Yoshinobu

× Kato, Yoshinobu

WEKO 92623

en Kato, Yoshinobu

Search repository
Yamamoto, Hiroshi

× Yamamoto, Hiroshi

WEKO 92624

en Yamamoto, Hiroshi

Search repository
Shikanai, Toshiharu

× Shikanai, Toshiharu

WEKO 92625

en Shikanai, Toshiharu

Search repository
Sugita, Mamoru

× Sugita, Mamoru

WEKO 92626

en Sugita, Mamoru

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
権利情報 This is the peer reviewed version of the following article: [Ito, A. , Sugita, C. , Ichinose, M. , Kato, Y. , Yamamoto, H. , Shikanai, T. and Sugita, M. (2018), An evolutionarily conserved P‐subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana. Plant J, 94: 638-648. doi:10.1111/tpj.13884], which has been published in final form at [https://doi.org/10.1111/tpj.13884]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
キーワード
主題Scheme Other
主題 P‐subfamily PPR protein
キーワード
主題Scheme Other
主題 splicing
キーワード
主題Scheme Other
主題 ndhA
キーワード
主題Scheme Other
主題 plastid
キーワード
主題Scheme Other
主題 Physcomitrella patens
キーワード
主題Scheme Other
主題 Arabidopsis thaliana
抄録
内容記述 Pentatricopeptide repeat (PPR) proteins are known to play important roles in post‐transcriptional regulation in plant organelles. However, the function of the majority of PPR proteins remains unknown. To examine their functions, Physcomitrella patens PpPPR_66 knockout (KO) mutants were generated and characterized. The KO mosses exhibited a wild‐type‐like growth phenotype but showed aberrant chlorophyll fluorescence due to defects in chloroplast NADH dehydrogenase‐like (NDH) activity. Immunoblot analysis suggested that disruption of PpPPR_66 led to a complete loss of the chloroplast NDH complex. To examine whether the loss of PpPPR_66 affects the expression of plastid ndh genes, the transcript levels of 11 plastid ndh genes were analyzed by reverse transcription PCR. This analysis indicated that splicing of the ndhA transcript was specifically impaired while mRNA accumulation levels as well as the processing patterns of other plastid ndh genes were not affected in the KO mutants. Complemented PpPPR_66 KO lines transformed with the PpPPR_66 full‐length cDNA rescued splicing of the ndhA transcript. Arabidopsis thaliana T‐DNA tagged lines of a PPR_66 homolog (At2 g35130) showed deficient splicing of the ndhA transcript. This indicates that the two proteins are functionally conserved between bryophytes and vascular plants. An in vitro RNA‐binding assay demonstrated that the recombinant PpPPR_66 bound preferentially to the region encompassing a part of exon 1 to a 5′ part of the ndhA group II intron. Taken together, these results indicate that PpPPR_66 acts as a specific factor to splice ndhA pre‐mRNA.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1111/tpj.13884
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0960-7412
書誌情報 en : The Plant Journal

巻 94, 号 4, p. 638-648, 発行日 2018-05
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