{"_buckets": {"deposit": "7721418e-f36c-4b10-bce8-a70533d1cc5b"}, "_deposit": {"id": "28351", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28351"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00028351"}, "control_number": "28351", "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "648", "bibliographicPageStart": "638", "bibliographicVolumeNumber": "94", "bibliographic_titles": [{"bibliographic_title": "The Plant Journal"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Pentatricopeptide repeat (PPR) proteins are known to play important roles in post\u2010transcriptional regulation in plant organelles. However, the function of the majority of PPR proteins remains unknown. To examine their functions, Physcomitrella patens PpPPR_66 knockout (KO) mutants were generated and characterized. The KO mosses exhibited a wild\u2010type\u2010like growth phenotype but showed aberrant chlorophyll fluorescence due to defects in chloroplast NADH dehydrogenase\u2010like (NDH) activity. Immunoblot analysis suggested that disruption of PpPPR_66 led to a complete loss of the chloroplast NDH complex. To examine whether the loss of PpPPR_66 affects the expression of plastid ndh genes, the transcript levels of 11 plastid ndh genes were analyzed by reverse transcription PCR. This analysis indicated that splicing of the ndhA transcript was specifically impaired while mRNA accumulation levels as well as the processing patterns of other plastid ndh genes were not affected in the KO mutants. Complemented PpPPR_66 KO lines transformed with the PpPPR_66 full\u2010length cDNA rescued splicing of the ndhA transcript. Arabidopsis thaliana T\u2010DNA tagged lines of a PPR_66 homolog (At2 g35130) showed deficient splicing of the ndhA transcript. This indicates that the two proteins are functionally conserved between bryophytes and vascular plants. An in vitro RNA\u2010binding assay demonstrated that the recombinant PpPPR_66 bound preferentially to the region encompassing a part of exon 1 to a 5\u2032 part of the ndhA group II intron. Taken together, these results indicate that PpPPR_66 acts as a specific factor to splice ndhA pre\u2010mRNA.", "subitem_description_type": "Abstract"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Wiley"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1111/tpj.13884", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\"This is the peer reviewed version of the following article: [Ito, A. , Sugita, C. , Ichinose, M. , Kato, Y. , Yamamoto, H. , Shikanai, T. and Sugita, M. (2018), An evolutionarily conserved P\u2010subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana. Plant J, 94: 638-648. doi:10.1111/tpj.13884], which has been published in final form at [https://doi.org/10.1111/tpj.13884]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.\""}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0960-7412", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "\u30a2\u30af\u30bb\u30b9\u6a29", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ito, Ayaka"}], "nameIdentifiers": [{"nameIdentifier": "92620", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugita, Chieko"}], "nameIdentifiers": [{"nameIdentifier": "92621", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ichinose, Mizuho"}], "nameIdentifiers": [{"nameIdentifier": "92622", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Yoshinobu"}], "nameIdentifiers": [{"nameIdentifier": "92623", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "92624", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shikanai, Toshiharu"}], "nameIdentifiers": [{"nameIdentifier": "92625", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugita, Mamoru"}], "nameIdentifiers": [{"nameIdentifier": "92626", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-11"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "TPJ-01005-2017.R1.pdf", "filesize": [{"value": "3.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3500000.0, "url": {"label": "TPJ-01005-2017.R1", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/28351/files/TPJ-01005-2017.R1.pdf"}, "version_id": "25c2f7f2-f0b6-44c8-b04b-b8bb1281864e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "P\u2010subfamily PPR protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "splicing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ndhA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "plastid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Physcomitrella patens", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Arabidopsis thaliana", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "An evolutionarily conserved P-subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "An evolutionarily conserved P-subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "17", "path": ["1931/1932/1933"], "permalink_uri": "http://hdl.handle.net/2237/00030545", "pubdate": {"attribute_name": "PubDate", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2019-07-11"}, "publish_date": "2019-07-11", "publish_status": "0", "recid": "28351", "relation": {}, "relation_version_is_last": true, "title": ["An evolutionarily conserved P-subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana"], "weko_shared_id": -1}