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Tanycyte-Like Cells Derived From Mouse Embryonic Stem Culture Show Hypothalamic Neural Stem/Progenitor Cell Functions
http://hdl.handle.net/2237/00030895
http://hdl.handle.net/2237/000308954d81d609-2604-43a0-911c-634ccd21f4b6
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-11-15 | |||||
タイトル | ||||||
タイトル | Tanycyte-Like Cells Derived From Mouse Embryonic Stem Culture Show Hypothalamic Neural Stem/Progenitor Cell Functions | |||||
言語 | en | |||||
著者 |
Kano, Mayuko
× Kano, Mayuko× Suga, Hidetaka× Ishihara, Takeshi× Sakakibara, Mayu× Soen, Mika× Yamada, Tomiko× Ozaki, Hajime× Mitsumoto, Kazuki× Kasai, Takatoshi× Sugiyama, Mariko× Onoue, Takeshi× Tsunekawa, Taku× Takagi, Hiroshi× Hagiwara, Daisuke× Ito, Yoshihiro× Iwama, Shintaro× Goto, Motomitsu× Banno, Ryoichi× Arima, Hiroshi |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is a pre-copyedited, author-produced version of an article accepted for publication in [Endocrinology] following peer review. The version of record [Endocrinology. v.160, n.7, 2019, p.1701-1718] is available online at: http://doi.org/10.1210/en.2019-00105. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mouse ES cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tanycytes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | retina and anterior neural fold homeobox | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adult neural stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hypothalamus | |||||
抄録 | ||||||
内容記述 | Tanycytes have recently been accepted as neural stem/progenitor cells in the postnatal hypothalamus. Persistent retina and anterior neural fold homeobox (Rax) expression is characteristic of tanycytes in contrast to its transient expression of whole hypothalamic precursors. In this study, we found that Rax^+ residual cells in the maturation phase of hypothalamic differentiation in mouse embryonic stem cell (mESC) cultures had similar characteristics to ventral tanycytes. They expressed typical neural stem/progenitor cell markers, including Sox2, vimentin, and nestin, and differentiated into mature neurons and glial cells. Quantitative RT-PCR analysis showed that Rax^+ residual cells expressed Fgf-10, Fgf-18, and Lhx2, which are expressed by ventral tanycytes. They highly expressed tanycyte-specific genes Dio2 and Gpr50 compared with Rax^+ early hypothalamic progenitor cells. Therefore, Rax^+ residual cells in the maturation phase of hypothalamic differentiation were considered to be more differentiated and similar to late progenitor cells and tanycytes. They self-renewed and formed neurospheres when cultured with exogenous FGF-2. Additionally, these Rax^+ neurospheres differentiated into three neuronal lineages (neurons, astrocytes, and oligodendrocytes), including neuropeptide Y^+ neuron, that are reported to be differentiated from ventral tanycytes toward the arcuate nuclei. Thus, Rax^+ residual cells were multipotent neural stem/progenitor cells. Rax^+ neurospheres were stably passaged and retained high Sox2 expression even after multiple passages. These results suggest the successful induction of Rax^+ tanycyte-like cells from mESCs [induced tanycyte-like (iTan) cells]. These hypothalamic neural stem/progenitor cells may have potential in regenerative medicine and as a research tool. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | ファイル公開日: 2020/07/01 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Oxford University Press | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1210/en.2019-00105 | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1945-7170 | |||||
書誌情報 |
en : Endocrinology 巻 160, 号 7, p. 1701-1718, 発行日 2019-07 |
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著者版フラグ | ||||||
値 | author |