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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Tanycyte-Like Cells Derived From Mouse Embryonic Stem Culture Show Hypothalamic Neural Stem/Progenitor Cell Functions

http://hdl.handle.net/2237/00030895
http://hdl.handle.net/2237/00030895
4d81d609-2604-43a0-911c-634ccd21f4b6
名前 / ファイル ライセンス アクション
en_2019-00105_R1.pdf en_2019-00105_R1 (997.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2019-11-15
タイトル
タイトル Tanycyte-Like Cells Derived From Mouse Embryonic Stem Culture Show Hypothalamic Neural Stem/Progenitor Cell Functions
言語 en
著者 Kano, Mayuko

× Kano, Mayuko

WEKO 94279

en Kano, Mayuko

Search repository
Suga, Hidetaka

× Suga, Hidetaka

WEKO 94280

en Suga, Hidetaka

Search repository
Ishihara, Takeshi

× Ishihara, Takeshi

WEKO 94281

en Ishihara, Takeshi

Search repository
Sakakibara, Mayu

× Sakakibara, Mayu

WEKO 94282

en Sakakibara, Mayu

Search repository
Soen, Mika

× Soen, Mika

WEKO 94283

en Soen, Mika

Search repository
Yamada, Tomiko

× Yamada, Tomiko

WEKO 94284

en Yamada, Tomiko

Search repository
Ozaki, Hajime

× Ozaki, Hajime

WEKO 94285

en Ozaki, Hajime

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Mitsumoto, Kazuki

× Mitsumoto, Kazuki

WEKO 94286

en Mitsumoto, Kazuki

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Kasai, Takatoshi

× Kasai, Takatoshi

WEKO 94287

en Kasai, Takatoshi

Search repository
Sugiyama, Mariko

× Sugiyama, Mariko

WEKO 94288

en Sugiyama, Mariko

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Onoue, Takeshi

× Onoue, Takeshi

WEKO 94289

en Onoue, Takeshi

Search repository
Tsunekawa, Taku

× Tsunekawa, Taku

WEKO 94290

en Tsunekawa, Taku

Search repository
Takagi, Hiroshi

× Takagi, Hiroshi

WEKO 94291

en Takagi, Hiroshi

Search repository
Hagiwara, Daisuke

× Hagiwara, Daisuke

WEKO 94292

en Hagiwara, Daisuke

Search repository
Ito, Yoshihiro

× Ito, Yoshihiro

WEKO 94293

en Ito, Yoshihiro

Search repository
Iwama, Shintaro

× Iwama, Shintaro

WEKO 94294

en Iwama, Shintaro

Search repository
Goto, Motomitsu

× Goto, Motomitsu

WEKO 94295

en Goto, Motomitsu

Search repository
Banno, Ryoichi

× Banno, Ryoichi

WEKO 94296

en Banno, Ryoichi

Search repository
Arima, Hiroshi

× Arima, Hiroshi

WEKO 94297

en Arima, Hiroshi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 This is a pre-copyedited, author-produced version of an article accepted for publication in [Endocrinology] following peer review. The version of record [Endocrinology. v.160, n.7, 2019, p.1701-1718] is available online at: http://doi.org/10.1210/en.2019-00105.
キーワード
主題Scheme Other
主題 Mouse ES cells
キーワード
主題Scheme Other
主題 tanycytes
キーワード
主題Scheme Other
主題 retina and anterior neural fold homeobox
キーワード
主題Scheme Other
主題 adult neural stem cells
キーワード
主題Scheme Other
主題 hypothalamus
抄録
内容記述 Tanycytes have recently been accepted as neural stem/progenitor cells in the postnatal hypothalamus. Persistent retina and anterior neural fold homeobox (Rax) expression is characteristic of tanycytes in contrast to its transient expression of whole hypothalamic precursors. In this study, we found that Rax^+ residual cells in the maturation phase of hypothalamic differentiation in mouse embryonic stem cell (mESC) cultures had similar characteristics to ventral tanycytes. They expressed typical neural stem/progenitor cell markers, including Sox2, vimentin, and nestin, and differentiated into mature neurons and glial cells. Quantitative RT-PCR analysis showed that Rax^+ residual cells expressed Fgf-10, Fgf-18, and Lhx2, which are expressed by ventral tanycytes. They highly expressed tanycyte-specific genes Dio2 and Gpr50 compared with Rax^+ early hypothalamic progenitor cells. Therefore, Rax^+ residual cells in the maturation phase of hypothalamic differentiation were considered to be more differentiated and similar to late progenitor cells and tanycytes. They self-renewed and formed neurospheres when cultured with exogenous FGF-2. Additionally, these Rax^+ neurospheres differentiated into three neuronal lineages (neurons, astrocytes, and oligodendrocytes), including neuropeptide Y^+ neuron, that are reported to be differentiated from ventral tanycytes toward the arcuate nuclei. Thus, Rax^+ residual cells were multipotent neural stem/progenitor cells. Rax^+ neurospheres were stably passaged and retained high Sox2 expression even after multiple passages. These results suggest the successful induction of Rax^+ tanycyte-like cells from mESCs [induced tanycyte-like (iTan) cells]. These hypothalamic neural stem/progenitor cells may have potential in regenerative medicine and as a research tool.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開日: 2020/07/01
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Oxford University Press
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1210/en.2019-00105
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 1945-7170
書誌情報 en : Endocrinology

巻 160, 号 7, p. 1701-1718, 発行日 2019-07
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