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  1. B100 理学部/理学研究科
  2. B100a 雑誌掲載論文
  3. 学術雑誌

Roles of maternal wnt8a transcripts in axis formation in zebrafish

http://hdl.handle.net/2237/00031579
http://hdl.handle.net/2237/00031579
cb93aa63-c1bc-44b7-ae75-703325ec8e6b
名前 / ファイル ライセンス アクション
Hino_Dev Hino_Dev Biol_2018 (1.8 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-03-11
タイトル
タイトル Roles of maternal wnt8a transcripts in axis formation in zebrafish
言語 en
著者 Hino, Hiromu

× Hino, Hiromu

WEKO 96513

en Hino, Hiromu

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Nakanishi, Akiko

× Nakanishi, Akiko

WEKO 96514

en Nakanishi, Akiko

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Seki, Ryoko

× Seki, Ryoko

WEKO 96515

en Seki, Ryoko

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Aoki, Tsubasa

× Aoki, Tsubasa

WEKO 96516

en Aoki, Tsubasa

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Yamaha, Etsuro

× Yamaha, Etsuro

WEKO 96517

en Yamaha, Etsuro

Search repository
Kawahara, Atsuo

× Kawahara, Atsuo

WEKO 96518

en Kawahara, Atsuo

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Shimizu, Takashi

× Shimizu, Takashi

WEKO 96519

en Shimizu, Takashi

Search repository
Hibi, Masahiko

× Hibi, Masahiko

WEKO 96520

en Hibi, Masahiko

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 wnt
キーワード
主題Scheme Other
主題 Axis formation
キーワード
主題Scheme Other
主題 Maternal mutant
キーワード
主題Scheme Other
主題 Zebrafish
抄録
内容記述 In early zebrafish development, the program for dorsal axis formation begins soon after fertilization. Previous studies suggested that dorsal determinants (DDs) localize to the vegetal pole, and are transported to the dorsal blastomeres in a microtubule-dependent manner. The DDs activate the canonical Wnt pathway and induce dorsal-specific genes that are required for dorsal axis formation. Among wnt-family genes, only the wnt8a mRNA is reported to localize to the vegetal pole in oocytes and to induce the dorsal axis, suggesting that Wnt8a is a candidate DD. Here, to reveal the roles of maternal wnt8a, we generated wnt8a mutants by transcription activator-like effector nucleases (TALENs), and established zygotic, maternal, and maternal zygotic wnt8a mutants by germ-line replacement. Zebrafish wnt8a has two open reading frames (ORF1 and ORF2) that are tandemly located in the genome. Although the zygotic ORF1 or ORF2 wnt8a mutants showed little or no axis-formation defects, the ORF1/2 compound mutants showed antero-dorsalized phenotypes, indicating that ORF1 and ORF2 have redundant roles in ventrolateral and posterior tissue formation. Unexpectedly, the maternal wnt8a ORF1/2 mutants showed no axis-formation defects. The maternal-zygotic wnt8a ORF1/2 mutants showed more severe antero-dorsalized phenotypes than the zygotic mutants. These results indicated that maternal wnt8a is dispensable for the initial dorsal determination, but cooperates with zygotic wnt8a for ventrolateral and posterior tissue formation. Finally, we re-examined the maternal wnt genes and found that Wnt6a is an alternative candidate DD.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.ydbio.2017.11.016
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0012-1606
書誌情報 en : Developmental Biology

巻 434, 号 1, p. 96-107, 発行日 2018-02-01
著者版フラグ
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