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  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch

http://hdl.handle.net/2237/00031580
7ed7ef07-bc37-4946-b635-e43e2aa6d349
名前 / ファイル ライセンス アクション
ForRepositoryNU.pdf ForRepositoryNU (3.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-03-11
タイトル
タイトル Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch
著者 Maeda, Eijiro

× Maeda, Eijiro

WEKO 96521

Maeda, Eijiro

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Atsumi, Yoshinori

× Atsumi, Yoshinori

WEKO 96522

Atsumi, Yoshinori

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Ishiguro, Mai

× Ishiguro, Mai

WEKO 96523

Ishiguro, Mai

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Nagayama, Kazuaki

× Nagayama, Kazuaki

WEKO 96524

Nagayama, Kazuaki

Search repository
Matsumoto, Takeo

× Matsumoto, Takeo

WEKO 96525

Matsumoto, Takeo

Search repository
権利
権利情報 © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 Bone marrow cells
キーワード
主題Scheme Other
主題 Multipotency
キーワード
主題Scheme Other
主題 Heterogeneity
キーワード
主題Scheme Other
主題 Cyclic stretch
抄録
内容記述 Multipotent stem cells are considered as a key material in regenerative medicine, and the understanding of the heterogeneity in the differentiation potentials of bone marrow-derived cells is important in the successful regenerative tissue repair. Therefore, the present study has been performed to investigate how the differentiation of post-harvest, native bone marrow-derived cells is regulated by cyclic stretch in vitro. Bone marrow-derived cells were obtained from mouse femur of both hind limbs and categorized into the following five categories: amebocytes, round cells, spindle cells, stellate cells and others. The cells were seeded on a silicone-made stretch chamber, and subjected to cyclic stretch with an amplitude of 10% at a frequency of 1 Hz for 7 days for cell shape analysis and for 3 days for the analysis of the expression of marker proteins of osteogenic (osteocalcin), vascular smooth muscle (α-smooth muscle actin and smooth muscle myosin heavy chain) and neurogenic (neurofilament) differentiation. When disregarding the differences in the cell shapes, there was an overall trend that the application of 10% cyclic stretch inhibited osteogenic and neurogenic differentiation, but enhanced smooth muscle differentiation. Close examinations revealed that round cells were influenced the most by cyclic stretch (significant up- or down-regulation in all the four marker protein expressions) while amebocytes and spindle cells were only influenced by cyclic stretch for vascular smooth muscle and/or neurogenic differentiation. As far as the authors know, this is the first study reporting the shape-related differences in the fate decision criteria for mechanical strain in bone marrow-derived cells.
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2020-11-11
内容記述タイプ Other
出版者
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jbiomech.2019.109371
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0021-9290
書誌情報 Journal of Biomechanics

巻 96, p. 109371, 発行日 2019-11-11
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