{"_buckets": {"deposit": "cba86c85-b626-4a14-8356-39d81315abec"}, "_deposit": {"id": "29393", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "29393"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00029393"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-11-11", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "109371", "bibliographicVolumeNumber": "96", "bibliographic_titles": [{"bibliographic_title": "Journal of Biomechanics"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Multipotent stem cells are considered as a key material in regenerative medicine, and the understanding of the heterogeneity in the differentiation potentials of bone marrow-derived cells is important in the successful regenerative tissue repair. Therefore, the present study has been performed to investigate how the differentiation of post-harvest, native bone marrow-derived cells is regulated by cyclic stretch in vitro. Bone marrow-derived cells were obtained from mouse femur of both hind limbs and categorized into the following five categories: amebocytes, round cells, spindle cells, stellate cells and others. The cells were seeded on a silicone-made stretch chamber, and subjected to cyclic stretch with an amplitude of 10% at a frequency of 1\u202fHz for 7\u202fdays for cell shape analysis and for 3\u202fdays for the analysis of the expression of marker proteins of osteogenic (osteocalcin), vascular smooth muscle (\u03b1-smooth muscle actin and smooth muscle myosin heavy chain) and neurogenic (neurofilament) differentiation. When disregarding the differences in the cell shapes, there was an overall trend that the application of 10% cyclic stretch inhibited osteogenic and neurogenic differentiation, but enhanced smooth muscle differentiation. Close examinations revealed that round cells were influenced the most by cyclic stretch (significant up- or down-regulation in all the four marker protein expressions) while amebocytes and spindle cells were only influenced by cyclic stretch for vascular smooth muscle and/or neurogenic differentiation. As far as the authors know, this is the first study reporting the shape-related differences in the fate decision criteria for mechanical strain in bone marrow-derived cells.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2020-11-11", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.jbiomech.2019.109371", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9290", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Maeda, Eijiro"}], "nameIdentifiers": [{"nameIdentifier": "96521", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Atsumi, Yoshinori"}], "nameIdentifiers": [{"nameIdentifier": "96522", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishiguro, Mai"}], "nameIdentifiers": [{"nameIdentifier": "96523", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagayama, Kazuaki"}], "nameIdentifiers": [{"nameIdentifier": "96524", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Takeo"}], "nameIdentifiers": [{"nameIdentifier": "96525", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-11"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ForRepositoryNU.pdf", "filesize": [{"value": "3.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3300000.0, "url": {"label": "ForRepositoryNU", "url": "https://nagoya.repo.nii.ac.jp/record/29393/files/ForRepositoryNU.pdf"}, "version_id": "e715fc47-5add-4a8c-9aa4-6e5ec6e4cd5f"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Bone marrow cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Multipotency", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Heterogeneity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cyclic stretch", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch"}]}, "item_type_id": "10", "owner": "1", "path": ["320/321/322"], "permalink_uri": "http://hdl.handle.net/2237/00031580", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2020-03-11"}, "publish_date": "2020-03-11", "publish_status": "0", "recid": "29393", "relation": {}, "relation_version_is_last": true, "title": ["Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch"], "weko_shared_id": null}