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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli

http://hdl.handle.net/2237/00031653
http://hdl.handle.net/2237/00031653
704ad98d-9303-4794-bdb1-e3209b93cc9e
名前 / ファイル ライセンス アクション
YggS-GCVfinal_version_1024.pdf YggS-GCVfinal_version_1024 (563.3 kB)
GCV-yggS_for_revise2-final.pdf GCV-yggS_for_revise2-final (440.4 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-03-19
タイトル
タイトル Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli
言語 en
著者 Ito, Tomokazu

× Ito, Tomokazu

WEKO 96824

en Ito, Tomokazu

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Hori, Ran

× Hori, Ran

WEKO 96825

en Hori, Ran

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Hemmi, Hisashi

× Hemmi, Hisashi

WEKO 96826

en Hemmi, Hisashi

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Downs, Diana M.

× Downs, Diana M.

WEKO 96827

en Downs, Diana M.

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Yoshimura, Tohru

× Yoshimura, Tohru

WEKO 96828

en Yoshimura, Tohru

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 This is the peer reviewed version of the following article: [Ito, T., Hori, R., Hemmi, H., Downs, D.M. and Yoshimura, T. (2020), Inhibition of glycine cleavage system by pyridoxine 5′‐phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli. Mol Microbiol, 113: 270-284. doi:10.1111/mmi.14415], which has been published in final form at [https://doi.org/10.1111/mmi.14415]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
抄録
内容記述 The YggS/Ybl036c/PLPBP family includes conserved pyridoxal 5′‐phosphate (PLP)‐binding proteins that play a critical role in the homeostasis of vitamin B6 and amino acids. Disruption of members of this family causes pleiotropic effects in many organisms by unknown mechanisms. In Escherichia coli, conditional lethality of the yggS and glyA (encoding serine hydroxymethyltransferase) has been described, but the mechanism of lethality was not determined. Strains lacking yggS and serA (3‐phosphoglycerate dehydrogenase) were conditionally lethality in the M9‐glucose medium supplemented with Gly. Analyses of vitamin B6 pools found the high‐levels of pyridoxine 5′‐phosphate (PNP) in the two yggS mutants. Growth defects of the double mutants could be eliminated by overexpressing PNP/PMP oxidase (PdxH) to decrease the PNP levels. Further, a serA pdxH strain, which accumulates PNP in the presence of yggS, exhibited similar phenotype to serA yggS mutant. Together these data suggested the inhibition of the glycine cleavage (GCV) system caused the synthetic lethality. Biochemical assays confirmed that PNP disrupts the GCV system by competing with PLP in GcvP protein. Our data are consistent with a model in which PNP‐dependent inhibition of the GCV system causes the conditional lethality observed in the glyA yggS or serA yggS mutants.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2021-02-06
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1111/mmi.14415
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0950-382X
書誌情報 en : Molecular Microbiology

巻 113, 号 1, p. 270-284, 発行日 2020-02-06
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