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Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli
http://hdl.handle.net/2237/00031653
http://hdl.handle.net/2237/00031653704ad98d-9303-4794-bdb1-e3209b93cc9e
| 名前 / ファイル | ライセンス | アクション |
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YggS-GCVfinal_version_1024 (563.3 kB)
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GCV-yggS_for_revise2-final (440.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-03-19 | |||||
| タイトル | ||||||
| タイトル | Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli | |||||
| 言語 | en | |||||
| 著者 |
Ito, Tomokazu
× Ito, Tomokazu× Hori, Ran× Hemmi, Hisashi× Downs, Diana M.× Yoshimura, Tohru |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | This is the peer reviewed version of the following article: [Ito, T., Hori, R., Hemmi, H., Downs, D.M. and Yoshimura, T. (2020), Inhibition of glycine cleavage system by pyridoxine 5′‐phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli. Mol Microbiol, 113: 270-284. doi:10.1111/mmi.14415], which has been published in final form at [https://doi.org/10.1111/mmi.14415]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The YggS/Ybl036c/PLPBP family includes conserved pyridoxal 5′‐phosphate (PLP)‐binding proteins that play a critical role in the homeostasis of vitamin B6 and amino acids. Disruption of members of this family causes pleiotropic effects in many organisms by unknown mechanisms. In Escherichia coli, conditional lethality of the yggS and glyA (encoding serine hydroxymethyltransferase) has been described, but the mechanism of lethality was not determined. Strains lacking yggS and serA (3‐phosphoglycerate dehydrogenase) were conditionally lethality in the M9‐glucose medium supplemented with Gly. Analyses of vitamin B6 pools found the high‐levels of pyridoxine 5′‐phosphate (PNP) in the two yggS mutants. Growth defects of the double mutants could be eliminated by overexpressing PNP/PMP oxidase (PdxH) to decrease the PNP levels. Further, a serA pdxH strain, which accumulates PNP in the presence of yggS, exhibited similar phenotype to serA yggS mutant. Together these data suggested the inhibition of the glycine cleavage (GCV) system caused the synthetic lethality. Biochemical assays confirmed that PNP disrupts the GCV system by competing with PLP in GcvP protein. Our data are consistent with a model in which PNP‐dependent inhibition of the GCV system causes the conditional lethality observed in the glyA yggS or serA yggS mutants. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ファイル公開：2021-02-06 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Wiley | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/mmi.14415 | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0950-382X | |||||
| 書誌情報 |
en : Molecular Microbiology 巻 113, 号 1, p. 270-284, 発行日 2020-02-06 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
