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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Benzoquinone synthesis-related genes of Tribolium castaneum confer the robust antifungal host defense to the adult beetles through the inhibition of conidial germination on the body surface

http://hdl.handle.net/2237/00032255
29e0da70-ed16-49c2-99b9-94ba07d5838e
名前 / ファイル ライセンス アクション
JIP_2019_242_Revision JIP_2019_242_Revision 1_V0 (1.7 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-05-26
タイトル
タイトル Benzoquinone synthesis-related genes of Tribolium castaneum confer the robust antifungal host defense to the adult beetles through the inhibition of conidial germination on the body surface
著者 Sawada, Mitsuki

× Sawada, Mitsuki

WEKO 99165

Sawada, Mitsuki

Search repository
Sano, Takuma

× Sano, Takuma

WEKO 99166

Sano, Takuma

Search repository
Hanakawa, Kento

× Hanakawa, Kento

WEKO 99167

Hanakawa, Kento

Search repository
Sirasoonthorn, Patchara

× Sirasoonthorn, Patchara

WEKO 99168

Sirasoonthorn, Patchara

Search repository
Oi, Takao

× Oi, Takao

WEKO 99169

Oi, Takao

Search repository
Miura, Ken

× Miura, Ken

WEKO 99170

Miura, Ken

Search repository
権利
権利情報 © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 Tribolium castaneum
キーワード
主題Scheme Other
主題 Immunity
キーワード
主題Scheme Other
主題 Entomopathogenic fungus
キーワード
主題Scheme Other
主題 Benzoquinone
キーワード
主題Scheme Other
主題 Scanning electron microscopy
抄録
内容記述 Insects fight against invading microbial pathogens through various immune-related measures that comprise ‘internal’, ‘external’ as well as ‘social’ immunities. The defenses by external immunity associated with the cuticular integument are supposed to be of particular importance in repelling entomopathogenic fungi that infect host insects transcutaneously. Among such integument-related defenses, external secretions of benzoquinone derivatives typical of tenebrionid beetles have been suggested to play important roles in the antimicrobial defenses. In the present study, by utilizing the experimental infection system composed of the red flour beetle Tribolium castaneum and generalist ascomycete entomopathogens Beauveria bassiana and Metarhizium anisopliae, we performed the functional assays of the three T. castaneum genes whose involvement in benzoquinone synthesis in the adults has been reported, namely GT39, GT62 and GT63. Observations by scanning electron microcopy (SEM) revealed that the conidia of the two fungal species did not germinate on the wild-type adult body surface but did on the pupae. The expression analyses demonstrated that the levels of GT39 and GT62 mRNA increased from middle pupae and reached high in early adults while GT63 did not show a clear adult-biased expression pattern. The RNA interference-based knockdown of any of the three genes in pupae resulted in the adults compromised to the infection of the both fungal species. SEM observations revealed that the gene silencing allowed the conidial germination on the body surface of the knockdown beetles, thereby impairing the robust antifungal defense of adult beetles. Thus, we have provided direct experimental evidence for the functional importance in vivo of these benzoquinone synthesis-related genes that support the antifungal defense of tenebrionid beetles.
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2021-01-01
内容記述タイプ Other
出版者
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jip.2019.107298
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0022-2011
書誌情報 Journal of Invertebrate Pathology

巻 169, p. 107298, 発行日 2020-01
著者版フラグ
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