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Differential effects of sevoflurane on the growth and apoptosis of human cancer cell lines
http://hdl.handle.net/2237/00032327
http://hdl.handle.net/2237/00032327f80b1358-8e3f-43c5-949d-d49e04d1afc3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JOAN-S-19-00301-2-05122019-konishi (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-10 | |||||
| タイトル | ||||||
| タイトル | Differential effects of sevoflurane on the growth and apoptosis of human cancer cell lines | |||||
| 言語 | en | |||||
| 著者 |
Hirai, Takahiro
× Hirai, Takahiro× Konishi, Yuko× Mizuno, Shoko× Rui, Zhou× Sun, Yao× Nishiwaki, Kimitoshi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | “This is a post-peer-review, pre-copyedit version of an article published in [Journal of Anesthesia]. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00540-019-02701-w”. | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Sevoflurane | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cancer | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Proliferation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Invasion | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Apoptosis | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: There have been contradictory findings regarding the effects of sevoflurane on the oncogenic properties of cancer cells. This study was conducted to gain insights into the fundamental rules governing the differential effects of sevoflurane exposure on various cancer cells derived from multiple origins. Methods: A series of cancer cell lines were exposed to 1% (v/v) sevoflurane for 2–8 h and then assessed for their proliferation, Matrigel invasion, and apoptotic cell death, in comparison with their untreated counterparts. Cell proliferation and Matrigel invasion assays were performed using Coulter counter and Boyden chamber techniques, respectively. Apoptosis was evaluated by staining cells with Annexin V and 7-AAD followed by fluorescence flow cytometry. In addition, the expression of cleaved caspase-3 protein, another marker of apoptosis, was assessed using immunoblotting. Results: Proliferation was significantly enhanced after sevoflurane exposure in six of eight cancer cell lines (NCI-H1299, MDA-MB-231, HCT116, DLD-1, HT29, and RKO). In contrast, sevoflurane attenuated proliferation in the last two cancer cell lines, A549 and MCF-7, as well as in the non-cancerous MCF10A cell line. Cell biological assays using four cancer cell lines demonstrated that accelerated but not attenuated cancer cell proliferation after sevoflurane exposure is associated with enhanced Matrigel invasion and suppressed apoptosis. Conclusion: Sevoflurane augmented or hampered cell proliferation and Matrigel invasion depending on the cancer cell line examined. Loss of sevoflurane-induced apoptosis occurring in cancer cell lines is likely to be correlated with their enhanced proliferation after sevoflurane exposure. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ファイル公開：2021/02/01 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Springer | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s00540-019-02701-w | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0913-8668 | |||||
| 書誌情報 |
en : Journal of Anesthesia 巻 34, 号 1, p. 47-57, 発行日 2020-02 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
