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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 82(3)

Identification of cisplatin-resistant factor by integration of transcriptomic and proteomic data using head and neck carcinoma cell lines

https://doi.org/10.18999/nagjms.82.3.519
https://doi.org/10.18999/nagjms.82.3.519
b1accb38-ff3f-446f-a8c5-8f7d7ac96148
名前 / ファイル ライセンス アクション
14_Inukai.pdf 14_Inukai.pdf (8.8 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2020-09-04
タイトル
タイトル Identification of cisplatin-resistant factor by integration of transcriptomic and proteomic data using head and neck carcinoma cell lines
著者 Inukai, Daisuke

× Inukai, Daisuke

WEKO 101080

Inukai, Daisuke

Search repository
Nishimura, Kunihiro

× Nishimura, Kunihiro

WEKO 101081

Nishimura, Kunihiro

Search repository
Okamoto, Hiroki

× Okamoto, Hiroki

WEKO 101082

Okamoto, Hiroki

Search repository
Sano, Rui

× Sano, Rui

WEKO 101083

Sano, Rui

Search repository
Ueda, Hiromi

× Ueda, Hiromi

WEKO 101084

Ueda, Hiromi

Search repository
Ota, Akinobu

× Ota, Akinobu

WEKO 101085

Ota, Akinobu

Search repository
Karnan, Sivasundaram

× Karnan, Sivasundaram

WEKO 101086

Karnan, Sivasundaram

Search repository
Hosokawa, Yoshitaka

× Hosokawa, Yoshitaka

WEKO 101087

Hosokawa, Yoshitaka

Search repository
Yoshikawa, Kazuhiro

× Yoshikawa, Kazuhiro

WEKO 101088

Yoshikawa, Kazuhiro

Search repository
Suzuki, Susumu

× Suzuki, Susumu

WEKO 101089

Suzuki, Susumu

Search repository
Ueda, Ryuzo

× Ueda, Ryuzo

WEKO 101090

Ueda, Ryuzo

Search repository
Murotani, Kenta

× Murotani, Kenta

WEKO 101091

Murotani, Kenta

Search repository
Bradford, Carol R.

× Bradford, Carol R.

WEKO 101092

Bradford, Carol R.

Search repository
Ogawa, Tetsuya

× Ogawa, Tetsuya

WEKO 101093

Ogawa, Tetsuya

Search repository
キーワード
主題Scheme Other
主題 S100A2
キーワード
主題Scheme Other
主題 transcriptomics
キーワード
主題Scheme Other
主題 proteomics
キーワード
主題Scheme Other
主題 cisplatin resistant factor
キーワード
主題Scheme Other
主題 head and neck squamous cell carcinoma
抄録
内容記述 Cisplatin is an important drug for the treatment of head and neck squamous cell carcinoma (HNSCC). Determining chemoresistant factors prior to treatment will lead to great benefits for clinicians and patients. Here, we evaluated chemoresistant factors by integrating proteomic and transcriptomic data using HNSCC cell lines to identify a more precise chemoresistant factor in HNSCC. We used four HNSCC cell lines: cisplatin-sensitive, acquired cisplatin resistance, naturally cisplatin-resistant, and acquired 5-FU resistance. Proteomic analysis was performed using iTRAQ, tandem mass spectrometry, and liquid chromatography-electrospray ionization-tandem mass spectrometry. Transcriptomic analysis was performed using microarrays. By integrating these independent data, common factors were addressed and functional analysis was performed using small interfering RNAs (siRNAs) to change the chemosensitivity. Using iTRAQ analysis, 7 proteins were identified as specific for cisplatin chemoresistance factors. Transcriptomic analysis revealed hundreds of potential candidate factors. By combining and integrating these data, S100A2 was identified as a potential cisplatin-specific chemoresistance factor. Functional analysis with siRNA revealed that the expression of S100A2 was reduced and cisplatin sensitivity recovered in the acquired and naturally cisplatin-resistant cell lines, but not in the cisplatin-sensitive cell lines. S100A2 was identified as a cisplatin-specific chemoresistance factor by integrating the transcriptomic and proteomic results obtained using HNSCC cell lines. This is a novel technique that allows for a precise identification, also known as a comprehensive analysis. Our findings indicate that these proteins could be used as biomarkers of HNSCC treatments, providing physicians with new treatment strategies for patients with HNSCC, showing chemoresistance.
内容記述タイプ Abstract
出版者
出版者 Nagoya University Graduate School of Medicine, School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.82.3.519
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/823.html
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 82, 号 3, p. 519-531, 発行日 2020-08
著者版フラグ
値 publisher
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