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Identification of cisplatin-resistant factor by integration of transcriptomic and proteomic data using head and neck carcinoma cell lines
https://doi.org/10.18999/nagjms.82.3.519
https://doi.org/10.18999/nagjms.82.3.519b1accb38-ff3f-446f-a8c5-8f7d7ac96148
名前 / ファイル | ライセンス | アクション |
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14_Inukai.pdf (8.8 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2020-09-04 | |||||
タイトル | ||||||
タイトル | Identification of cisplatin-resistant factor by integration of transcriptomic and proteomic data using head and neck carcinoma cell lines | |||||
言語 | en | |||||
著者 |
Inukai, Daisuke
× Inukai, Daisuke× Nishimura, Kunihiro× Okamoto, Hiroki× Sano, Rui× Ueda, Hiromi× Ota, Akinobu× Karnan, Sivasundaram× Hosokawa, Yoshitaka× Yoshikawa, Kazuhiro× Suzuki, Susumu× Ueda, Ryuzo× Murotani, Kenta× Bradford, Carol R.× Ogawa, Tetsuya |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報Resource | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
権利情報 | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | S100A2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transcriptomics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proteomics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cisplatin resistant factor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | head and neck squamous cell carcinoma | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cisplatin is an important drug for the treatment of head and neck squamous cell carcinoma (HNSCC). Determining chemoresistant factors prior to treatment will lead to great benefits for clinicians and patients. Here, we evaluated chemoresistant factors by integrating proteomic and transcriptomic data using HNSCC cell lines to identify a more precise chemoresistant factor in HNSCC. We used four HNSCC cell lines: cisplatin-sensitive, acquired cisplatin resistance, naturally cisplatin-resistant, and acquired 5-FU resistance. Proteomic analysis was performed using iTRAQ, tandem mass spectrometry, and liquid chromatography-electrospray ionization-tandem mass spectrometry. Transcriptomic analysis was performed using microarrays. By integrating these independent data, common factors were addressed and functional analysis was performed using small interfering RNAs (siRNAs) to change the chemosensitivity. Using iTRAQ analysis, 7 proteins were identified as specific for cisplatin chemoresistance factors. Transcriptomic analysis revealed hundreds of potential candidate factors. By combining and integrating these data, S100A2 was identified as a potential cisplatin-specific chemoresistance factor. Functional analysis with siRNA revealed that the expression of S100A2 was reduced and cisplatin sensitivity recovered in the acquired and naturally cisplatin-resistant cell lines, but not in the cisplatin-sensitive cell lines. S100A2 was identified as a cisplatin-specific chemoresistance factor by integrating the transcriptomic and proteomic results obtained using HNSCC cell lines. This is a novel technique that allows for a precise identification, also known as a comprehensive analysis. Our findings indicate that these proteins could be used as biomarkers of HNSCC treatments, providing physicians with new treatment strategies for patients with HNSCC, showing chemoresistance. | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | Nagoya University Graduate School of Medicine, School of Medicine | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.82.3.519 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/823.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 82, 号 3, p. 519-531, 発行日 2020-08 |
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著者版フラグ | ||||||
値 | publisher |
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Cite as
Inukai, Daisuke, Nishimura, Kunihiro, Okamoto, Hiroki, Sano, Rui, Ueda, Hiromi, Ota, Akinobu, Karnan, Sivasundaram, Hosokawa, Yoshitaka, Yoshikawa, Kazuhiro, Suzuki, Susumu, Ueda, Ryuzo, Murotani, Kenta, Bradford, Carol R., Ogawa, Tetsuya, 2020, Identification of cisplatin-resistant factor by integration of transcriptomic and proteomic data using head and neck carcinoma cell lines: Nagoya University Graduate School of Medicine, School of Medicine, 519–531 p.
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