{"_buckets": {"deposit": "8bf52d38-d442-498d-ad64-9344875a05c1"}, "_deposit": {"id": "30449", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "30449"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00030449"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-05-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageStart": "e49890", "bibliographicVolumeNumber": "21", "bibliographic_titles": [{"bibliographic_title": "EMBO reports"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "RNA processing occurs co\u2010transcriptionally through the dynamic recruitment of RNA processing factors to RNA polymerase II (RNAPII). However, transcriptome\u2010wide identification of protein\u2013RNA interactions specifically assembled on transcribing RNAPII is challenging. Here, we develop the targeted RNA immunoprecipitation sequencing (tRIP\u2010seq) method that detects protein\u2013RNA interaction sites in thousands of cells. The high sensitivity of tRIP\u2010seq enables identification of protein\u2013RNA interactions at functional subcellular levels. Application of tRIP\u2010seq to the FUS\u2010RNA complex in the RNAPII machinery reveals that FUS binds upstream of alternative polyadenylation (APA) sites of nascent RNA bound to RNAPII, which retards RNAPII and suppresses the recognition of the polyadenylation signal by CPSF. Further tRIP\u2010seq analyses demonstrate that the repression of APA is achieved by a complex composed of FUS and U1 snRNP on RNAPII, but not by either one alone. Moreover, our analysis reveals that FUS mutations in familial amyotrophic lateral sclerosis (ALS) that impair the FUS\u2010U1 snRNP interaction aberrantly activate the APA sites. tRIP\u2010seq provides new insights into the regulatory mechanism of co\u2010transcriptional RNA processing by RNA processing factors.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2020-11-06", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "EMBO Press"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.15252/embr.201949890", "subitem_relation_type_select": "DOI"}}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "1469-221X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Masuda, Akio"}], "nameIdentifiers": [{"nameIdentifier": "101234", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawachi, Toshihiko"}], "nameIdentifiers": [{"nameIdentifier": "101235", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeda, Jun\u2010ichi"}], "nameIdentifiers": [{"nameIdentifier": "101236", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohkawara, Bisei"}], "nameIdentifiers": [{"nameIdentifier": "101237", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Mikako"}], "nameIdentifiers": [{"nameIdentifier": "101238", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohno, Kinji"}], "nameIdentifiers": [{"nameIdentifier": "101239", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "EMBOR-2019-49890-T_manuscript.pdf", "filesize": [{"value": "974.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 974500.0, "url": {"label": "EMBOR-2019-49890-T_manuscript", "url": "https://nagoya.repo.nii.ac.jp/record/30449/files/EMBOR-2019-49890-T_manuscript.pdf"}, "version_id": "0b07cd83-5d7b-4620-b623-e4339024fa1e"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "tRIP\u2010seq reveals repression of premature polyadenylation by co\u2010transcriptional FUS\u2010U1 snRNP assembly", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "tRIP\u2010seq reveals repression of premature polyadenylation by co\u2010transcriptional FUS\u2010U1 snRNP assembly"}]}, "item_type_id": "10", "owner": "1", "path": ["499/500/501"], "permalink_uri": "http://hdl.handle.net/2237/00032634", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2020-09-15"}, "publish_date": "2020-09-15", "publish_status": "0", "recid": "30449", "relation": {}, "relation_version_is_last": true, "title": ["tRIP\u2010seq reveals repression of premature polyadenylation by co\u2010transcriptional FUS\u2010U1 snRNP assembly"], "weko_shared_id": null}