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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

tRIP‐seq reveals repression of premature polyadenylation by co‐transcriptional FUS‐U1 snRNP assembly

http://hdl.handle.net/2237/00032634
http://hdl.handle.net/2237/00032634
0e2d6a0c-e75f-4b4a-943e-780886df2908
名前 / ファイル ライセンス アクション
EMBOR-2019-49890-T_manuscript.pdf EMBOR-2019-49890-T_manuscript (974.5 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-09-15
タイトル
タイトル tRIP‐seq reveals repression of premature polyadenylation by co‐transcriptional FUS‐U1 snRNP assembly
言語 en
著者 Masuda, Akio

× Masuda, Akio

WEKO 101234

en Masuda, Akio

Search repository
Kawachi, Toshihiko

× Kawachi, Toshihiko

WEKO 101235

en Kawachi, Toshihiko

Search repository
Takeda, Jun‐ichi

× Takeda, Jun‐ichi

WEKO 101236

en Takeda, Jun‐ichi

Search repository
Ohkawara, Bisei

× Ohkawara, Bisei

WEKO 101237

en Ohkawara, Bisei

Search repository
Ito, Mikako

× Ito, Mikako

WEKO 101238

en Ito, Mikako

Search repository
Ohno, Kinji

× Ohno, Kinji

WEKO 101239

en Ohno, Kinji

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
抄録
内容記述 RNA processing occurs co‐transcriptionally through the dynamic recruitment of RNA processing factors to RNA polymerase II (RNAPII). However, transcriptome‐wide identification of protein–RNA interactions specifically assembled on transcribing RNAPII is challenging. Here, we develop the targeted RNA immunoprecipitation sequencing (tRIP‐seq) method that detects protein–RNA interaction sites in thousands of cells. The high sensitivity of tRIP‐seq enables identification of protein–RNA interactions at functional subcellular levels. Application of tRIP‐seq to the FUS‐RNA complex in the RNAPII machinery reveals that FUS binds upstream of alternative polyadenylation (APA) sites of nascent RNA bound to RNAPII, which retards RNAPII and suppresses the recognition of the polyadenylation signal by CPSF. Further tRIP‐seq analyses demonstrate that the repression of APA is achieved by a complex composed of FUS and U1 snRNP on RNAPII, but not by either one alone. Moreover, our analysis reveals that FUS mutations in familial amyotrophic lateral sclerosis (ALS) that impair the FUS‐U1 snRNP interaction aberrantly activate the APA sites. tRIP‐seq provides new insights into the regulatory mechanism of co‐transcriptional RNA processing by RNA processing factors.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2020-11-06
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 EMBO Press
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.15252/embr.201949890
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 1469-221X
書誌情報 en : EMBO reports

巻 21, 号 5, p. e49890, 発行日 2020-05-06
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