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Expression profiles of genes for enzymes involved in capsidiol production in Nicotiana benthamiana
http://hdl.handle.net/2237/00033202
http://hdl.handle.net/2237/00033202a4e69e8c-4d28-4ceb-af4d-8a96300c7b73
名前 / ファイル | ライセンス | アクション |
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Rin_et_al_2020JGPP (17.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-15 | |||||
タイトル | ||||||
タイトル | Expression profiles of genes for enzymes involved in capsidiol production in Nicotiana benthamiana | |||||
言語 | en | |||||
著者 |
Rin, Soriya
× Rin, Soriya× Imano, Sayaka× Camagna, Maurizio× Suzuki, Takamasa× Tanaka, Aiko× Sato, Ikuo× Chiba, Sotaro× Kawakita, Kazuhito× Takemoto, Daigo |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | “This is a post-peer-review, pre-copyedit version of an article published in [Journal of General Plant Pathology]. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10327-020-00931-5”. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MAP kinases | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mevalonate pathway | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nicotiana benthamiana | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phytophthora infestans | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sesquiterpenoid phytoalexins | |||||
抄録 | ||||||
内容記述 | In Solanaceae plants, the major phytoalexins produced during the induction of plant defense are sesquiterpenoids, such as capsidiol for Nicotiana species and rishitin for Solanum species, which are produced via the mevalonate (MVA) pathway. Eight enzymes are involved in the production of farnesyl pyrophosphate (FPP), the common precursor of phytosterols for maintaining membrane integrity and sesquiterpenoid phytoalexins for plant defense. In this study, expression profiles of N. benthamiana genes for the production of capsidiol during the induction of disease resistance were investigated. In the genome of N. benthamiana, multiple copies of genes for each enzyme in the MVA pathway were identified, and the expression of some, but not all MVA genes, was significantly upregulated after inoculation with Phytophthora infestans, or treatment with the INF1 elicitor, a secretory protein of P. infestans. For genes encoding enzymes involved in capsidiol production, 10 copies of 5-epi-aristolochene synthase (NbEAS) and six copies of 5-epi-aristolochene dihydroxylase (NbEAH) were identified, and all copies were significantly upregulated during the induction of disease resistance. Gene silencing of MAP kinase genes NbWIPK, NbSIPK, and NbNTF4 compromised INF1-induced production of phytoalexins. Expression analysis of control and NbWIPK/SIPK/NTF4-silenced plants indicated that most of the MVA genes are not under the control of these MAP kinases. In contrast, the expression pattern of NbWIPK/SIPK/NTF4 and all copies of NbEAH genes showed significant correlation, suggesting that MAP kinases are critical regulators of transcriptional upregulation of specific genes for capsidiol production. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Springer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s10327-020-00931-5 | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1345-2630 | |||||
書誌情報 |
en : Journal of General Plant Pathology 巻 86, 号 5, p. 340-349, 発行日 2020-09 |
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著者版フラグ | ||||||
値 | author |