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  1. A500 情報学部/情報学研究科・情報文化学部・情報科学研究科
  2. A500a 雑誌掲載論文
  3. 学術雑誌

Bioluminescence burst caused by a process in carbohydrate metabolism in a luciferase reporter strain of Escherichia coli

http://hdl.handle.net/2237/00033491
http://hdl.handle.net/2237/00033491
2bf782dd-72b8-4400-b199-59a8e0a4a4b7
名前 / ファイル ライセンス アクション
Aibaetal_BBRC_200804.pdf Aibaetal_BBRC_200804 (1.6 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2021-02-08
タイトル
タイトル Bioluminescence burst caused by a process in carbohydrate metabolism in a luciferase reporter strain of Escherichia coli
言語 en
著者 Aiba, Kazuki

× Aiba, Kazuki

WEKO 103288

en Aiba, Kazuki

Search repository
Kobayashi, Yuto

× Kobayashi, Yuto

WEKO 103289

en Kobayashi, Yuto

Search repository
Aoki, Setsuyuki

× Aoki, Setsuyuki

WEKO 103290

en Aoki, Setsuyuki

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
抄録
内容記述 We previously reported that Escherichia coli strains carrying a firefly luciferase reporter gene (luc+) showed a posttranslationally-generated bioluminescence burst upon entry into the stationary phase. In this paper, we studied the mechanism underpinning this burst by using a series of “Keio” gene deletion strains. When luc+ driven by the lac gene promoter (lacp::luc+) was introduced into a group of Keio strains, the resulting reporter strains showed significantly altered timing and/or sizes of the burst. Remarkably, a reporter strain that lacked phosphoglucose isomerase (PGI), which catalyzes the second step of glycolysis, showed no burst, while the onset of the stationary phase of this strain was the same as that of the wild-type (WT) reporter strain. Consistently, the WT reporter strain showed no burst, when grown on arabinose or xylose instead of glucose as the carbon source. These results suggest that a process in carbohydrate metabolism is involved in the mechanism of generation of the burst. We measured temporal changes in intracellular NADPH concentrations but could not detect a significant increase or decrease relative to the occurrence of the burst. Functional implications and possible applications of the burst are discussed.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2021-11-05
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.bbrc.2020.08.016
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0006-291X
書誌情報 en : Biochemical and Biophysical Research Communications

巻 532, 号 2, p. 185-189, 発行日 2020-11-05
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