{"_buckets": {"deposit": "653a6674-33a3-414f-81a2-2381e6ffb53c"}, "_deposit": {"id": "31321", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "31321"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00031321", "sets": ["1937"]}, "author_link": ["103360", "103361", "103362", "103363", "103364", "103365", "103366", "103367", "103368", "103369"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-09-17", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageEnd": "1198.e5", "bibliographicPageStart": "1192", "bibliographicVolumeNumber": "27", "bibliographic_titles": [{"bibliographic_title": "Cell Chemical Biology", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cryptochrome 1 (CRY1) and CRY2 are core regulators of the circadian clock, and the development of isoform-selective modulators is important for the elucidation of their redundant and distinct functions. Here, we report the identification and functional characterization of a small-molecule modulator of the mammalian circadian clock that selectively controls CRY1. Cell-based circadian chemical screening identified a thienopyrimidine derivative KL201 that lengthened the period of circadian rhythms in cells and tissues. Functional assays revealed stabilization of CRY1 but not CRY2 by KL201. A structure-activity relationship study of KL201 derivatives in combination with X-ray crystallography of the CRY1-KL201 complex uncovered critical sites and interactions required for CRY1 regulation. KL201 bound to CRY1 in overlap with FBXL3, a subunit of ubiquitin ligase complex, and the effect of KL201 was blunted by knockdown of FBXL3. KL201 will facilitate isoform-selective regulation of CRY1 to accelerate chronobiology research and therapeutics against clock-related diseases.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "ファイル公開：2021-09-17", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.chembiol.2020.05.008", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN（print）", "attribute_value_mlt": [{"subitem_source_identifier": "2451-9456", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Miller, Simon", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103360", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aikawa, Yoshiki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103361", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugiyama, Akiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103362", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagai, Yoshiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103363", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hara, Aya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103364", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oshima, Tsuyoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103365", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Amaike, Kazuma", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103366", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kay, Steve A.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103367", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itami, Kenichiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103368", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirota, Tsuyoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "103369", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-09-17"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CellChemBiol-rev2-Hirota.pdf", "filesize": [{"value": "4.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 4800000.0, "url": {"label": "CellChemBiol-rev2-Hirota", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/31321/files/CellChemBiol-rev2-Hirota.pdf"}, "version_id": "704857f2-c370-4bae-9d58-51d34a8120c7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "circadian clock", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cryptochrome", "subitem_subject_scheme": "Other"}, {"subitem_subject": "small-molecule modulator", "subitem_subject_scheme": "Other"}, {"subitem_subject": "chemical biology", "subitem_subject_scheme": "Other"}, {"subitem_subject": "X-ray crystallography", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "An Isoform-Selective Modulator of Cryptochrome 1 Regulates Circadian Rhythms in Mammals", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "An Isoform-Selective Modulator of Cryptochrome 1 Regulates Circadian Rhythms in Mammals", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["1937"], "permalink_uri": "http://hdl.handle.net/2237/00033501", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2021-02-09"}, "publish_date": "2021-02-09", "publish_status": "0", "recid": "31321", "relation": {}, "relation_version_is_last": true, "title": ["An Isoform-Selective Modulator of Cryptochrome 1 Regulates Circadian Rhythms in Mammals"], "weko_shared_id": -1}