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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Involvement of the transcription factor E75 in adult cuticular formation in the red flour beetle Tribolium castaneum

http://hdl.handle.net/2237/00033512
http://hdl.handle.net/2237/00033512
5c514ce1-1b74-4286-b264-afa39b92ed11
名前 / ファイル ライセンス アクション
Sapin_et_al.pdf Sapin_et_al (1.1 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2021-02-10
タイトル
タイトル Involvement of the transcription factor E75 in adult cuticular formation in the red flour beetle Tribolium castaneum
言語 en
著者 Sapin, Gelyn D.

× Sapin, Gelyn D.

WEKO 103434

en Sapin, Gelyn D.

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Tomoda, Kai

× Tomoda, Kai

WEKO 103435

en Tomoda, Kai

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Tanaka, Sayumi

× Tanaka, Sayumi

WEKO 103436

en Tanaka, Sayumi

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Shinoda, Tetsuro

× Shinoda, Tetsuro

WEKO 103437

en Shinoda, Tetsuro

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Miura, Ken

× Miura, Ken

WEKO 103438

en Miura, Ken

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Minakuchi, Chieka

× Minakuchi, Chieka

WEKO 103439

en Minakuchi, Chieka

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
抄録
内容記述 Insect adult metamorphosis generally proceeds with undetectable levels of juvenile hormone (JH). In adult development of the red flour beetle Tribolium castaneum, biosynthesis of adult cuticle followed by its pigmentation and sclerotization occurs, and dark coloration of the cuticle becomes visible in pharate adults. Here, we examined the molecular mechanism of adult cuticular formation in more detail. We noticed that an exogenous JH mimic (JHM) treatment of Day 0 pupae did not inhibit pigmentation or sclerotization, but instead, induced precocious pigmentation of adult cuticle two days in advance. Quantitative RT-PCR analyses revealed that ecdysone-induced protein 75B (E75) is downregulated in JHM-treated pupae. Meanwhile, tyrosine hydroxylase (Th), an enzyme involved in cuticular pigmentation and sclerotization, was precociously induced, whereas a structural cuticular protein CPR27 was downregulated, by exogenous JHM treatment. RNA interference-mediated knockdown of E75 resulted in precocious adult cuticular pigmentation, which resembled the phenotype caused by JHM treatment. Notably, upregulation of Th as well as suppression of CPR27 were observed with E75 knockdown. Meanwhile, JHM treatment suppressed the expression of genes involved in melanin synthesis, such as Yellow-y and Laccase 2, but E75 knockdown did not result in marked reduction in their expression. Taken together, these results provided insights into the regulatory mechanisms of adult cuticular formation; the transcription of genes involved in adult cuticular formation proceeds in a proper timing with undetectable JH, and exogenous JHM treatment disturbs their transcription. For some of these genes such as Th and CPR27, E75 is involved in transcriptional regulation. This study shed light on the molecular mode of action of JHM as insecticides; exogenous JHM treatment disturbed the expression of genes involved in the adult cuticular formation, which resulted in lethality as pharate adults.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2021-11-01
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.ibmb.2020.103450
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0965-1748
書誌情報 en : Insect Biochemistry and Molecular Biology

巻 126, p. 103450, 発行日 2020-11
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