{"_buckets": {"deposit": "51848810-c9f8-49f9-b3e0-bc19d37b1ca4"}, "_deposit": {"id": "31336", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "31336"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00031336"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "104017", "bibliographicVolumeNumber": "121", "bibliographic_titles": [{"bibliographic_title": "Journal of Insect Physiology"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Insect juvenile hormone (JH) is well known to regulate post-embryonic development and reproduction in concert with ecdysteroids in a variety of insect species. In contrast, our knowledge on the role of JH in embryonic development is limited and inconsistent. Preceding studies indicate that JH biosynthesis or JH signaling genes are dispensable in holometabolous Drosophila melanogaster and Bombyx mori, while essential in hemimetabolous Blattella germanica. In the red flour beetle Tribolium castaneum, we performed functional analyses of key factors in JH signaling, i.e. the JH receptor Methoprene-tolerant (Met) and the early JH-response gene Kr\u00fcppel homolog 1 (Kr-h1) using parental RNA interference. Knockdown of Met resulted in a significant reduction in hatching rates and survival rates in the first and second larval instars. Meanwhile, knockdown of Kr-h1 caused no significant effect on hatching or survival. The unhatched embryos under Met knockdown developed up to the late embryonic stage, but their body shape was flat and tubby compared with the controls. Attempts to suppress JH biosynthesis by parental RNA interference of JH biosynthetic enzymes were unsuccessful due to insufficient knockdown efficiency. These results suggested that Met but not Kr-h1 is essential for the embryonic development of T. castaneum, although involvement of JH still remains to be examined. Taken together, the function of Met in embryonic development seems to be diverse among insect species.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "Available online 20 January 2020.", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.jinsphys.2020.104017", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0022-1910", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Naruse, Shouya"}], "nameIdentifiers": [{"nameIdentifier": "103457", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Washidu, Yumiko"}], "nameIdentifiers": [{"nameIdentifier": "103458", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miura, Ken"}], "nameIdentifiers": [{"nameIdentifier": "103459", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shinoda, Tetsuro"}], "nameIdentifiers": [{"nameIdentifier": "103460", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Minakuchi, Chieka"}], "nameIdentifiers": [{"nameIdentifier": "103461", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-02-10"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Naruse et al__maintext.pdf", "filesize": [{"value": "263.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 263700.0, "url": {"label": "Naruse et al__maintext", "url": "https://nagoya.repo.nii.ac.jp/record/31336/files/Naruse et al__maintext.pdf"}, "version_id": "b7e18a3a-bf8b-475d-9bce-f6f95d3c2fbd"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-02-10"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "Naruse et al_Figs\u0026Tables.pdf", "filesize": [{"value": "4.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 4500000.0, "url": {"label": "Naruse et al_Figs\u0026Tables", "url": "https://nagoya.repo.nii.ac.jp/record/31336/files/Naruse et al_Figs\u0026Tables.pdf"}, "version_id": "acb667dd-e0c0-4f78-b2ee-30819d7b6770"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Juvenile hormone", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Methoprene-tolerant", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Embryonic development: Tribolium castaneum", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Methoprene-tolerant is essential for embryonic development of the red flour beetle Tribolium castaneum", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Methoprene-tolerant is essential for embryonic development of the red flour beetle Tribolium castaneum"}]}, "item_type_id": "10", "owner": "1", "path": ["643/666/667"], "permalink_uri": "http://hdl.handle.net/2237/00033516", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2021-02-10"}, "publish_date": "2021-02-10", "publish_status": "0", "recid": "31336", "relation": {}, "relation_version_is_last": true, "title": ["Methoprene-tolerant is essential for embryonic development of the red flour beetle Tribolium castaneum"], "weko_shared_id": null}