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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 66(3-4)

Cell Death of Human Oral Squamous Cell Carcinoma Cell Line Induced by Herpes Simplex Virus Thymidine Kinase Gene and Ganciclovir

https://doi.org/10.18999/nagjms.66.3-4.129
https://doi.org/10.18999/nagjms.66.3-4.129
931303ff-1fe2-42a7-96e3-f4baacefc168
名前 / ファイル ライセンス アクション
nj6636.pdf nj6636.pdf (125.9 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2006-02-10
タイトル
タイトル Cell Death of Human Oral Squamous Cell Carcinoma Cell Line Induced by Herpes Simplex Virus Thymidine Kinase Gene and Ganciclovir
著者 Nishikawa, Masaya

× Nishikawa, Masaya

WEKO 10141

Nishikawa, Masaya

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Hayashi, Yasushi

× Hayashi, Yasushi

WEKO 10142

Hayashi, Yasushi

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Yamamoto, Noriyuki

× Yamamoto, Noriyuki

WEKO 10143

Yamamoto, Noriyuki

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Fukui, Takafumi

× Fukui, Takafumi

WEKO 10144

Fukui, Takafumi

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Fukuhara, Hirokazu

× Fukuhara, Hirokazu

WEKO 10145

Fukuhara, Hirokazu

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Mitsudo, Kenji

× Mitsudo, Kenji

WEKO 10146

Mitsudo, Kenji

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Tohnai, Iwai

× Tohnai, Iwai

WEKO 10147

Tohnai, Iwai

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Ueda, Minoru

× Ueda, Minoru

WEKO 10148

Ueda, Minoru

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Mizuno, Masaaki

× Mizuno, Masaaki

WEKO 10149

Mizuno, Masaaki

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Yoshida, Jun

× Yoshida, Jun

WEKO 10150

Yoshida, Jun

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キーワード
主題Scheme Other
主題 Adenovirus
キーワード
主題Scheme Other
主題 HSVtk
キーワード
主題Scheme Other
主題 Suicide gene therapy
キーワード
主題Scheme Other
主題 Head and neck squamous cell carcinoma
キーワード
主題Scheme Other
主題 Apoptosis
キーワード
主題Scheme Other
主題 Safety
抄録
内容記述 Suicide gene therapy combining herpes simplex virus thymidine kinase gene (HSVtk) and ganciclovir (GCV) is one strategy for the treatment of head and neck squamous cell carcinoma (HNSCC). The purpose of this study is to determine the mechanism of cell death that occurs in suicide gene therapy using HSVtk and GCV and to assess the safety of that therapy. The human oral squamous cell carcinoma cell line SAS was treated with adenovirus vector containing HSVtk gene (AdHSVtk) and GCV in vitro. Morphological changes including chromatin condensation, cell shrinkage, blebbing of cell membrane, and ballooning formations were observed. Changes in the localization of phospholipids in the cell membrane were also observed. The results of flow cytometry showed a maximum of about 65% of cells in the early phase of apoptosis. In addition, DNA fragmentation was investigated using the TUNEL method in vivo. Nude mice (BALB/c AJD-nu-, aged 4 weeks) were implanted with SAS and treated with AdHSVtk and GCV. Tumor sections were then observed. The treatment group was confirmed to have DNA fragmentation-positive cells. These results suggest that suicide gene therapy using AdHSVtk and GCV led to apoptosis of the oral squamous cell carcinoma cell line.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.66.3-4.129
ID登録タイプ JaLC
書誌情報 Nagoya Journal of Medical Science

巻 66, 号 3-4, p. 129-137, 発行日 2003-11
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/5397
識別子タイプ HDL
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