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Cell Death of Human Oral Squamous Cell Carcinoma Cell Line Induced by Herpes Simplex Virus Thymidine Kinase Gene and Ganciclovir
https://doi.org/10.18999/nagjms.66.3-4.129
https://doi.org/10.18999/nagjms.66.3-4.129931303ff-1fe2-42a7-96e3-f4baacefc168
名前 / ファイル | ライセンス | アクション |
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nj6636.pdf (125.9 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-02-10 | |||||
タイトル | ||||||
タイトル | Cell Death of Human Oral Squamous Cell Carcinoma Cell Line Induced by Herpes Simplex Virus Thymidine Kinase Gene and Ganciclovir | |||||
言語 | en | |||||
著者 |
Nishikawa, Masaya
× Nishikawa, Masaya× Hayashi, Yasushi× Yamamoto, Noriyuki× Fukui, Takafumi× Fukuhara, Hirokazu× Mitsudo, Kenji× Tohnai, Iwai× Ueda, Minoru× Mizuno, Masaaki× Yoshida, Jun |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adenovirus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HSVtk | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Suicide gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Head and neck squamous cell carcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Apoptosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Safety | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Suicide gene therapy combining herpes simplex virus thymidine kinase gene (HSVtk) and ganciclovir (GCV) is one strategy for the treatment of head and neck squamous cell carcinoma (HNSCC). The purpose of this study is to determine the mechanism of cell death that occurs in suicide gene therapy using HSVtk and GCV and to assess the safety of that therapy. The human oral squamous cell carcinoma cell line SAS was treated with adenovirus vector containing HSVtk gene (AdHSVtk) and GCV in vitro. Morphological changes including chromatin condensation, cell shrinkage, blebbing of cell membrane, and ballooning formations were observed. Changes in the localization of phospholipids in the cell membrane were also observed. The results of flow cytometry showed a maximum of about 65% of cells in the early phase of apoptosis. In addition, DNA fragmentation was investigated using the TUNEL method in vivo. Nude mice (BALB/c AJD-nu-, aged 4 weeks) were implanted with SAS and treated with AdHSVtk and GCV. Tumor sections were then observed. The treatment group was confirmed to have DNA fragmentation-positive cells. These results suggest that suicide gene therapy using AdHSVtk and GCV led to apoptosis of the oral squamous cell carcinoma cell line. | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | Nagoya University School of Medicine | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.66.3-4.129 | |||||
ID登録タイプ | JaLC | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 66, 号 3-4, p. 129-137, 発行日 2003-11 |
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フォーマット | ||||||
値 | application/pdf | |||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/5397 | |||||
識別子タイプ | HDL |