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Although slice culture, which is one of the tissue or organ culture methods, effectively preserves the in vivo microenvironment where normal developmental processes occur, conventional phase-contrast microscopic observation of brain slices fails to provide good visibility of single cells. However, a combination of slice culture with the use of fl uorescent dyes and/or the introduction of fl uorescent protein genes provides live, three-dimensional information on cytogenetic and histogenetic events at the individual cell level. Dynamic cellular behaviors can then be vividly captured without destroying tissue structures.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/5406"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.67.3-4.65", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine"}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Miyata, Takaki"}], "nameIdentifiers": [{"nameIdentifier": "10183", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito, Kanako"}], "nameIdentifiers": [{"nameIdentifier": "10184", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishizawa, Yuji"}], "nameIdentifiers": [{"nameIdentifier": "10185", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murayama, Ayako"}], "nameIdentifiers": [{"nameIdentifier": "10186", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Masaoka, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "10187", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogawa, Masaharu"}], "nameIdentifiers": [{"nameIdentifier": "10188", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "p65MIYATA.pdf", "filesize": [{"value": "336.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 336100.0, "url": {"label": "p65MIYATA.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/3973/files/p65MIYATA.pdf"}, "version_id": "f2373821-bf9a-4350-8a73-621f249f4074"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "brain", "subitem_subject_scheme": "Other"}, {"subitem_subject": "development", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tissue culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cell migration", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Modern slice culture for direct observation of production and migration of brain neurons", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Modern slice culture for direct observation of production and migration of brain neurons"}]}, "item_type_id": "9", "owner": "1", "path": ["520"], "permalink_uri": "https://doi.org/10.18999/nagjms.67.3-4.65", "pubdate": {"attribute_name": "公開日", "attribute_value": "2006-02-10"}, "publish_date": "2006-02-10", "publish_status": "0", "recid": "3973", "relation": {}, "relation_version_is_last": true, "title": ["Modern slice culture for direct observation of production and migration of brain neurons"], "weko_shared_id": 3}