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Suicide gene therapy using adenovirus vector for human oral squamous carcinoma cell line In vitro
https://doi.org/10.18999/nagjms.67.3-4.83
https://doi.org/10.18999/nagjms.67.3-4.831b12b53f-8e3b-4148-aaaa-f82653b6187d
名前 / ファイル | ライセンス | アクション |
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p83YAMAMOTO.pdf (367.2 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-02-10 | |||||
タイトル | ||||||
タイトル | Suicide gene therapy using adenovirus vector for human oral squamous carcinoma cell line In vitro | |||||
言語 | en | |||||
著者 |
Yamamoto, Noriyuki
× Yamamoto, Noriyuki× Hayashi, Yasushi× Kagami, Hideaki× Fukui, Takafumi× Fukuhara, Hirokazu× Tohnai, Iwai× Ueda, Minoru× Mizuno, Masaaki× Yoshida, Jun |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Squamous Cell Carcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adenovirus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Herpes Simplex Virus Thymidine Kinase Gene, | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ganciclovir | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bystander effect | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recently, suicide gene therapy using the herpes simplex virus thymidine kinase (HSVtk) gene followed by ganciclovir (GCV) administration was evaluated for the treatment of cancer. The purpose of this study was to investigate the effectiveness of suicide gene therapy using the replication-defi cient recombinant adenovirus vector for human oral squamous carcinoma cell lines. To evaluate transduction effi ciency, each cell line was transduced in vitro with an adenovirus vector containing the β-galactosidase gene. By 24 hours after transduction, nearly 100% of the cells were transduced at a multiplicity of infection (MOI) of 10, and from 30 to 10% at an MOI of 1. Next, each cell line was transduced with an adenovirus vector containing the HSVtk gene, and a subsequent administration of GCV for the assessment of suicide gene therapy. A subsequent administration of GCV resulted in complete tumor cell death. In addition, we conducted a morphological analysis of that cell death using video-enhanced contrast differential interference contrast microscopy, and we observed that it included both apoptosis and necrosis after HSVtk gene and GCV treatment. These results suggest that adenovirus-mediated suicide gene therapy induced remarkable cytotoxicity with a bystander effect in human oral squamous cell carcinoma thus suggesting an effective treatment strategy for that tumor. | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | Nagoya University School of Medicine | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.67.3-4.83 | |||||
ID登録タイプ | JaLC | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 67, 号 3-4, p. 83-91, 発行日 2005-06 |
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フォーマット | ||||||
値 | application/pdf | |||||
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値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/5408 | |||||
識別子タイプ | HDL |