{"_buckets": {"deposit": "f6fd17b5-43f4-4392-9346-f1de42d36c43"}, "_deposit": {"id": "5108", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5108"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00005108", "sets": ["667"]}, "author_link": ["12640", "12641", "12642", "12643"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "403", "bibliographicPageStart": "393", "bibliographicVolumeNumber": "1708", "bibliographic_titles": [{"bibliographic_title": "Biochimica et Biophysica Acta", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The H+-pyrophosphatase (H+-PPase) consists of a single polypeptide, containing 16 or 17 transmembrane domains. To determine the higher order oligomeric state of Streptomyces coelicolor H+-PPase, we constructed a series of cysteine substitution mutants and expressed them in Escherichia coli. Firstly, we analyzed the formation of disulfide bonds, promoted by copper, in mutants with single cysteine substitutions. 28 of 39 mutants formed disulfide bonds, including S545C, a substitution at the periplasmic side. The formation of intermolecular disulfide bonds suppressed the enzyme activity of several, where the substituted residues were located in the cytosol. Creating disulfide links in the cytosol may interfere with the enzyme’s catalytic function. Secondly, we prepared double mutants by introducing second cysteine substitutions into the S545C mutant. These double-cysteine mutants produced cross-linked complexes, estimated to be at least tetramers and possibly hexamers. Thirdly, we co-expressed epitope-tagged, wild type and inactive mutant H+-PPases in E. coli and confirmed the formation of oligomers by co-purifying one subunit using the epitope tag used to label the other. The enzyme activity of these oligomers was markedly suppressed. We propose that H+-PPase is present as an oligomer made up of at least two or three sets of dimers.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/6661"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mimura, Hisatoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "12640", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakanishi, Yoichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "12641", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maeshima, Masayoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "12642", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "前島, 正義", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "12643", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2005-BBA-ScPP.pdf", "filesize": [{"value": "918.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 918300.0, "url": {"label": "2005-BBA-ScPP.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/5108/files/2005-BBA-ScPP.pdf"}, "version_id": "65a18e8b-6e03-4ae6-9895-3d06eff16d58"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Disulfide bond", "subitem_subject_scheme": "Other"}, {"subitem_subject": "H+-pyrophosphatase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Oligomerization", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Proton pump", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Oligomerization of H+-pyrophosphatase and its structural and functional consequences", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Oligomerization of H+-pyrophosphatase and its structural and functional consequences", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/6661", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2006-06-30"}, "publish_date": "2006-06-30", "publish_status": "0", "recid": "5108", "relation": {}, "relation_version_is_last": true, "title": ["Oligomerization of H+-pyrophosphatase and its structural and functional consequences"], "weko_shared_id": -1}