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et a1. (1) have shown that, when F-actin is mixed with inert high polymer, a large number of actin filaments closely align in parallel with overlaps to form a long and thick bundle. The bundle may be designated non-polar, as the constituent filaments are random in polarity (1). I prepared non-polar bundles of F-actin using methylcellulose (MC) as the high polymer, exposed them to heavy meromyosin (HMM) in the presence of ATP under a light microscope, and followed their morphological changes in the continuous presence of MC. It was found that bundles several tens of micrometers long contracted to about one-third the initial length, while becoming thicker, in half a minute after exposure to HMM. Subsequently, each bundle was split longitudinally into several bundles in a stepwise manner, while the newly formed ones remained associated together at one of the two ends. The product, an aster-like assembly of actin bundles, was morphologically quiescent; that is, individual bundles never contracted upon second exposure to HMM and ATP, although they were still longer than the F-actin used. Bundles in this state consisted of filaments with parallel polarity as examined by electron microscopy. This implies that non-polar bundles were transformed into assemblies of polar bundles with ATP hydrosis by HMM. Importantly, myosin subfragment-1 caused neither contraction nor transformation. These results are interpreted as follows. In the presence of ATP, the two-headed HMM molecule was able to cross-bridge antiparallel actin filaments, as well as parallel ones. 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  1. B100 理学部／理学研究科
  2. B100d 学位論文
  3. 博士論文(理博・論理博)

ヘビーメロミオシンによって引き起こされる極性が反平行なアクチン線維間の滑り運動

http://hdl.handle.net/2237/6898
4758e258-99ea-4d1e-b33e-3d51fa541014
名前 / ファイル ライセンス アクション
ko2436_abstr.pdf ko2436_abstr.pdf (164.7 kB)
ko2436.pdf ko2436.pdf (6.7 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2006-09-07
タイトル
タイトル ヘビーメロミオシンによって引き起こされる極性が反平行なアクチン線維間の滑り運動
その他のタイトル
その他のタイトル Heavy Meromyosin lnduces Sliding Movements Between Antiparallel Actin Filaments
著者 瀧口, 金吾

× 瀧口, 金吾

WEKO 13480

瀧口, 金吾
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Takiguchi, Kingo

× Takiguchi, Kingo

WEKO 13481

Takiguchi, Kingo
Search repository
抄録
内容記述 鈴木らによって、Ｆ－アクチン溶液に高分子を添加すると浸透圧効果により多数のアクチン線維が互いにオーバーラップしながら並行に集まり、長く太い束を形成することが報告されている［１］。束内のアクチン線維の極性はランダムであるため、この束は極性を持たない［１］。高分子としてメチルセルロースを用いて形成させた束は、長さ数10μmに及びアクチン線維1本の長さの10倍以上になる。この束にATP存在下でヘビーメロミオシン(HMM)あるいはミオシンサブフラグメント1(S-1)を加えて、その形状の変化を光学顕微鏡で観察した。HMMを加えた場合、まず束に収縮が起こる。収縮により、束は30秒程度で初めの長さの約3分の1になり、同時に太くなる。この収縮を経た束は、やがて数本の小さな束に裂けて分かれる。但し、これらの束は各の一端で結合し合うので、星型をした数本の束の集団が形成される。星型集団を構成する各束は、長さがアクチン線維より長い場合でも、HMMとATPの再添加によって収縮しなかった。これらの束を電子顕微鏡で観察したところ、束内のアクチン線維の極性は平行であった。従って、初め極性を持たなかった1本の束が、最後に極性を持つ束の星型集団に変化したことになる。一方、S-1を用いた場合には、束の収縮も星型構造の形成も起きなかった。以上の結果を次のように解釈している。双頭構造を持つHMMは、極性が平行な2本のアクチン線維とのみならず極性が反平行な2本とも同時に相互作用することができる。反平行な場合は2本のアクチン線維間に滑りを引き起こす。平行な場合はHMMは外からこの2本のアクチン線維間に加わる滑り力に対する抵抗を生む。Suzuki et a1. (1) have shown that, when F-actin is mixed with inert high polymer, a large number of actin filaments closely align in parallel with overlaps to form a long and thick bundle. The bundle may be designated non-polar, as the constituent filaments are random in polarity (1). I prepared non-polar bundles of F-actin using methylcellulose (MC) as the high polymer, exposed them to heavy meromyosin (HMM) in the presence of ATP under a light microscope, and followed their morphological changes in the continuous presence of MC. It was found that bundles several tens of micrometers long contracted to about one-third the initial length, while becoming thicker, in half a minute after exposure to HMM. Subsequently, each bundle was split longitudinally into several bundles in a stepwise manner, while the newly formed ones remained associated together at one of the two ends. The product, an aster-like assembly of actin bundles, was morphologically quiescent; that is, individual bundles never contracted upon second exposure to HMM and ATP, although they were still longer than the F-actin used. Bundles in this state consisted of filaments with parallel polarity as examined by electron microscopy. This implies that non-polar bundles were transformed into assemblies of polar bundles with ATP hydrosis by HMM. Importantly, myosin subfragment-1 caused neither contraction nor transformation. These results are interpreted as follows. In the presence of ATP, the two-headed HMM molecule was able to cross-bridge antiparallel actin filaments, as well as parallel ones. In the antiparallel case, HMM induced sliding forces between the two filaments, while, in the parallel case, HMM traveled along the pair directionally, producing resistence against external sliding forces acting upon the two filaments.
内容記述タイプ Abstract
内容記述
内容記述 名古屋大学博士学位論文 学位の種類:理学博士 (課程) 学位授与年月日:平成3年3月25日
内容記述タイプ Other
言語
言語 jpn
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
書誌情報 発行日 1991-03-25
学位授与年度
学位授与年度 1990
学位授与年月日
学位授与年月日 1991-03-25
学位授与番号
学位授与番号 13901甲第2436号
フォーマット
application/pdf
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/6898
識別子タイプ HDL
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