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  1. F100 環境医学研究所
  2. F100a 雑誌掲載論文
  3. 学術雑誌

Diminished climing fiber innervation of Purkinje cells in the cerebellum of myosin Va mutant mice and rats

http://hdl.handle.net/2237/7904
4eb39538-488b-42e0-949b-507cfcda286d
名前 / ファイル ライセンス アクション
Fig7.pdf Fig7.pdf (259.9 kB)
Fig6.pdf Fig6.pdf (1.9 MB)
Fig5.pdf Fig5.pdf (298.4 kB)
Fig4.pdf Fig4.pdf (282.8 kB)
Fig3.pdf Fig3.pdf (572.8 kB)
Fig2.pdf Fig2.pdf (302.2 kB)
Fig1.pdf Fig1.pdf (150.1 kB)
Tabel2.pdf Tabel2.pdf (44.6 kB)
Table1.pdf Table1.pdf (16.4 kB)
Takagishi_et_al_doc.pdf Takagishi_et_al_doc.pdf (198.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-05-25
タイトル
タイトル Diminished climing fiber innervation of Purkinje cells in the cerebellum of myosin Va mutant mice and rats
著者 Takagishi, Yoshiko

× Takagishi, Yoshiko

WEKO 16876

Takagishi, Yoshiko

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Hashimoto, Kouichi

× Hashimoto, Kouichi

WEKO 16877

Hashimoto, Kouichi

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Kayahara, Tetsuro

× Kayahara, Tetsuro

WEKO 16878

Kayahara, Tetsuro

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Watanabe, Masahiko

× Watanabe, Masahiko

WEKO 16879

Watanabe, Masahiko

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Otsuka, Hiroyuki

× Otsuka, Hiroyuki

WEKO 16880

Otsuka, Hiroyuki

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Mizoguchi, Akira

× Mizoguchi, Akira

WEKO 16881

Mizoguchi, Akira

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Kano, Masanobu

× Kano, Masanobu

WEKO 16882

Kano, Masanobu

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Murata, Yoshiharu

× Murata, Yoshiharu

WEKO 16883

Murata, Yoshiharu

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キーワード
主題Scheme Other
主題 myosin Va
キーワード
主題Scheme Other
主題 cerebellum
キーワード
主題Scheme Other
主題 climbing fiber
キーワード
主題Scheme Other
主題 development
キーワード
主題Scheme Other
主題 Purkinje cell
キーワード
主題Scheme Other
主題 axonal transport
キーワード
主題Scheme Other
主題 Ca^2+ signaling
キーワード
主題Scheme Other
主題 mutant mouse
抄録
内容記述 Myosin Va is an actin-based molecular motor that is involved in organelle transport and membrane trafficking. Here, we explored the role of myosin Va in the formation of synaptic circuitry by examining climbing fiber (CF) innervation of Purkinje cells (PCs) in the cerebella of dilute-neurological (d-n) mice and dilute-opisthotonus (dop) rats that have mutations in dilute–encoded myosin Va. Anterograde labeling of CFs with biotinylated dextran amine (BDA) revealed that they arborized poorly and that their tips extended only half way through the thickness of the molecular layer (ML) in adult d-n mice. Using immunohistochemistry specific for vesicular glutamate transporter 2 (VGluT2) to visualize CF synaptic terminals, we found that during development and in adulthood, these terminals did not ascend as far along the proximal shaft dendrites of PCs in d-n mice and dop rats as they did in normal animals. An irregular distribution of BDA-labeled bulbous varicosities and VGluT2 spots along CF branches were also noted in these animals. Finally, VGluT2-positive CF terminals were occasionally localized on the PC somata of adult d-n cerebella. These phenotypes are consistent with our electrophysiological findings that CF-mediated excitatory postsynaptic currents (EPSCs) were significantly smaller in amplitude and faster in decay in adult d-n mice, and that the regression of multiple CFs was slightly delayed in developing d-n mice. Taken together, our results suggest that myosin Va is essential for terminal CF extension and for the establishment of CF synapses within the proper dendritic territories of PCs.
内容記述タイプ Abstract
内容記述
内容記述 Running title: Climbing fibers in myosin Va mutants
内容記述タイプ Other
出版者
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
DOI
関連識別子
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1002/dneu.20375
書誌情報 Developmental Neurobiology

巻 67, 号 7, p. 909-923, 発行日 2007-06
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著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/7904
識別子タイプ HDL
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