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  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Speciation of Small Molecules and Inorganic Ions in Salmon Egg Cell Cytoplasm by Surfactant-Mediated HPLC/ICP-MS

http://hdl.handle.net/2237/8748
http://hdl.handle.net/2237/8748
be821bc6-964b-452c-8cd2-bb5fc96d9db1
名前 / ファイル ライセンス アクション
v19_n1_117-121.pdf v19_n1_117-121.pdf (83.1 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-09-14
タイトル
タイトル Speciation of Small Molecules and Inorganic Ions in Salmon Egg Cell Cytoplasm by Surfactant-Mediated HPLC/ICP-MS
言語 en
著者 MATSUURA, Hirotaka

× MATSUURA, Hirotaka

WEKO 19074

en MATSUURA, Hirotaka

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HASEGAWA, Takuya

× HASEGAWA, Takuya

WEKO 19075

en HASEGAWA, Takuya

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NAGATA, Hitomi

× NAGATA, Hitomi

WEKO 19076

en NAGATA, Hitomi

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TAKATANI, Kohei

× TAKATANI, Kohei

WEKO 19077

en TAKATANI, Kohei

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ASANO, Motoki

× ASANO, Motoki

WEKO 19078

en ASANO, Motoki

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ITOH, Akihide

× ITOH, Akihide

WEKO 19079

en ITOH, Akihide

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HARAGUCHI, Hiroki

× HARAGUCHI, Hiroki

WEKO 19080

en HARAGUCHI, Hiroki

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
抄録
内容記述タイプ Abstract
内容記述 The speciation of diverse elements in salmon egg cell cytoplasm was performed by a surfactant-mediated HPLC/ICP-MS hyphenated system. In the present experiment, an ODS column coated with CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), which is a zwitterionic bile acid derivative, was employed as a surfactant-mediated separation column, and ICP-MS was used as an element-selective detector. The present surfactant-mediated HPLC allowed us to separate large and small molecules within 10 min; large molecules, such as proteins, were eluted within 2.5 min, while small molecules were eluted after 2.5 min, but within 10 min. In the present experiment, Fe, Cu, and Zn in egg cell cytoplasm were observed mostly in species with large molecular weights, indicating that these elements are contained as metalloproteins or metalloenzymes in egg cell cytoplasm. On the contrary, it was found that P, S, Mo, and halogens in egg cell cytoplasm were contained as small molecules or inorganic ions. The major species of P in egg cell cytoplasm was identified as the phosphate ion (PO_4^3-). Molybdenum, Cl, and Br in egg cell cytoplasm were molybdate (MoO_4^2-), chloride (Cl^-), and bromide (Br^-) ions, respectively.
言語 en
出版者
出版者 日本分析化学会
言語 ja
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.2116/analsci.19.117
ISSN
収録物識別子タイプ PISSN
収録物識別子 0910-6340
書誌情報 en : Analytical Sciences

巻 19, 号 1, p. 117-121, 発行日 2003-07
フォーマット
値 application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/8748
識別子タイプ HDL
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