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Speciation of Small Molecules and Inorganic Ions in Salmon Egg Cell Cytoplasm by Surfactant-Mediated HPLC/ICP-MS
http://hdl.handle.net/2237/8748
http://hdl.handle.net/2237/8748be821bc6-964b-452c-8cd2-bb5fc96d9db1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
v19_n1_117-121.pdf (83.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-09-14 | |||||
| タイトル | ||||||
| タイトル | Speciation of Small Molecules and Inorganic Ions in Salmon Egg Cell Cytoplasm by Surfactant-Mediated HPLC/ICP-MS | |||||
| 言語 | en | |||||
| 著者 |
MATSUURA, Hirotaka
× MATSUURA, Hirotaka× HASEGAWA, Takuya× NAGATA, Hitomi× TAKATANI, Kohei× ASANO, Motoki× ITOH, Akihide× HARAGUCHI, Hiroki |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The speciation of diverse elements in salmon egg cell cytoplasm was performed by a surfactant-mediated HPLC/ICP-MS hyphenated system. In the present experiment, an ODS column coated with CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), which is a zwitterionic bile acid derivative, was employed as a surfactant-mediated separation column, and ICP-MS was used as an element-selective detector. The present surfactant-mediated HPLC allowed us to separate large and small molecules within 10 min; large molecules, such as proteins, were eluted within 2.5 min, while small molecules were eluted after 2.5 min, but within 10 min. In the present experiment, Fe, Cu, and Zn in egg cell cytoplasm were observed mostly in species with large molecular weights, indicating that these elements are contained as metalloproteins or metalloenzymes in egg cell cytoplasm. On the contrary, it was found that P, S, Mo, and halogens in egg cell cytoplasm were contained as small molecules or inorganic ions. The major species of P in egg cell cytoplasm was identified as the phosphate ion (PO_4^3-). Molybdenum, Cl, and Br in egg cell cytoplasm were molybdate (MoO_4^2-), chloride (Cl^-), and bromide (Br^-) ions, respectively. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | 日本分析化学会 | |||||
| 言語 | ja | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.2116/analsci.19.117 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0910-6340 | |||||
| 書誌情報 |
en : Analytical Sciences 巻 19, 号 1, p. 117-121, 発行日 2003-07 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/8748 | |||||
| 識別子タイプ | HDL | |||||
