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Altered expression of barley proline transporter causes different growth responses in Arabidopsis
http://hdl.handle.net/2237/8780
http://hdl.handle.net/2237/8780ba6efe43-85f2-49b4-98a2-29b34e14ad34
名前 / ファイル | ライセンス | アクション |
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PLANTA.pdf (479.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-09-26 | |||||
タイトル | ||||||
タイトル | Altered expression of barley proline transporter causes different growth responses in Arabidopsis | |||||
言語 | en | |||||
著者 |
UEDA, Akihiro
× UEDA, Akihiro× SHI, Weiming× SHIMADA, Takiko× MIYAKE, Hiroshi× TAKABE, Tetsuko |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arabidopsis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Barley | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HvProT | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Proline | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Proline transporter | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Root cap | |||||
抄録 | ||||||
内容記述 | A compatible solute, proline is accumulated in various kinds of plants and microorganisms under environmental stresses. The function of proline is thought to be an osmotic regulator under water stress, and its transport into cells is mediated by a proline transporter. Here, we report the effects of expressing the barley proline transporter (HvProT) under the control of either the CaMV35S promoter (35Sp) or a root cap promoter (RCp), on Arabidopsis growth. In Arabidopsis, transformed HvProT functions in the plasma membrane, like other amino acid transporters. Reduction in biomass production was observed in aerial parts of 35Sp-HvProT plants, and it was accompanied with decreased proline accumulation in leaves. Impaired growth of 35Sp-HvProT plants was restored by exogenously adding L-proline. These results suggested that growth reduction was caused by a deficiency of endogenous proline. In 35Sp-HvProT plants, the amount of proline dehydrogenase (PDH) transcript was increased compared to wild type plants, with a consequent enhancement of the activity of PDH. On the other hand, transgenic RCp-HvProT plants accumulated 2- to 3-fold more proline in the root tip region compared to WT, and root elongation was enhanced at the same time. Thus, different physiological responses were caused by the different location in accumulation of proline using two different promoters for heterologous expression of HvProT. These results indicate the importance of proline distribution at the tissue level during vegetative development. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | The original publication is available at www.springerlink.com. | |||||
言語 | en | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Springer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00425-007-0615-y | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1432-2048 | |||||
書誌情報 |
en : Planta 巻 227, 号 2, p. 277-286, 発行日 2007 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/8780 | |||||
識別子タイプ | HDL |