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  1. B100 理学部/理学研究科
  2. B100a 雑誌掲載論文
  3. 学術雑誌

Tension-Dependent Formation of Stress Fibers in Fibroblasts : A Study Using Semi-Intact Cells

http://hdl.handle.net/2237/9066
http://hdl.handle.net/2237/9066
421daed3-1cb8-429b-8b63-8dfe63411b02
名前 / ファイル ライセンス アクション
JSME_C47-4-962.pdf JSME_C47-4-962.pdf (1.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-12-03
タイトル
タイトル Tension-Dependent Formation of Stress Fibers in Fibroblasts : A Study Using Semi-Intact Cells
言語 en
著者 HIRATA, Hiroaki

× HIRATA, Hiroaki

WEKO 20552

en HIRATA, Hiroaki

Search repository
TATSUMI, Hitoshi

× TATSUMI, Hitoshi

WEKO 20553

en TATSUMI, Hitoshi

Search repository
SOKABE, Masahiro

× SOKABE, Masahiro

WEKO 20554

en SOKABE, Masahiro

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 ja
権利情報 日本機械学会
権利
言語 ja
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである。
キーワード
主題Scheme Other
主題 Biomechanics
キーワード
主題Scheme Other
主題 Image Processing
キーワード
主題Scheme Other
主題 Optical Measurement
キーワード
主題Scheme Other
主題 Stress Fiber
キーワード
主題Scheme Other
主題 Semi-Intact Cell
キーワード
主題Scheme Other
主題 Traction Force
キーワード
主題Scheme Other
主題 Molecular Machine
キーワード
主題Scheme Other
主題 Self-Organization
抄録
内容記述 Stress fiber is a bundle of action filaments, which also contains myosin and cross-linking proteins. It remains unclear how stress fibers are formed from their main components, actin and myosin. To address this question we examined the process of formation of stress fibers in a model system, a semi-intact cell. Fibroblasts were treated with a Rho kinase inhibitor to disorganize stress fibers. These cells were permeabilized and used as semi-intact cells in this study. When these cells were treated with ATP and Ca2+, stress fibers were restored within 15 min. Motion analysis of action filaments labeled with quantum dots during formation of stress fibers revealed action flaments were gradually assembled while they were moving toward the center of the cdell. This suggests that ATP-driven tension influences the stress fiber formation. The ATP-driven tension was mimicked in this study by artificial centripetal traction force, which was carried out by dragging the micropipette attached on the cell surface. Stress fibers were formed by the traction force application. These results suggest that tension in actomyosin meshwork is an important element in stress fiber formation.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 The Japan Society of Mechanical Engineers
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/110004820824/
ISSN
収録物識別子タイプ PISSN
収録物識別子 1344-7653
書誌情報 en : JSME International Journal Series C

巻 47, 号 4, p. 962-969, 発行日 2004-12
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/9066
識別子タイプ HDL
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