ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "0ca621d9-b7cd-48df-a38f-b1614c0bf26e"}, "_deposit": {"id": "7569", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "7569"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00007569", "sets": ["322"]}, "author_link": ["21324", "21325", "21326", "21327"], "control_number": "7569", "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "1137", "bibliographicPageStart": "1131", "bibliographicVolumeNumber": "81", "bibliographic_titles": [{"bibliographic_title": "Photochemistry and Photobiology", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In the current studies, we examined the effects of hexagonal lattice formation with lipid membranes on the structural stability of native bacteriorhodopsin(bR). Denaturation kinetic measurements for bR solubilized with the mild nonionic detergent, Triton X-100(TX100), were performed in the dark and under illumination by visible light. The solubilized bR was stable in the dark over a wide concentration range of TX100(1 to 200 mM). In purple membranes, a bilobed band was observed in visible circular dichroic spectra due to interactions between neighboring chromophores. At all concentrations of TX100, this was replaced by a single positive band. Upon illumination with visible light, TX100-solubilized bR clearly showed photobleaching to bacterioopsin. These experimental results suggest that photobleaching is due to a lack of intermolecular interactions inside the purple membrane lattice. Extensive kinetic measurements further revealed that the rate constant of photobleaching is strongly dependent on the detergent concentration, although the activation energy for photobleaching does not significantly change with the TX100 concentration. The mechanism of photobleaching for the solubilized bR is discussed with respect to detergent micelle properties.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/9268"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Blackwell", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1562/2005-03-22-RA-470", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "The definitive version is available at www.blackwell-synergy.com", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0031-8655", "subitem_source_identifier_type": "PISSN"}]}, "item_10_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sasaki, Takanori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "21324", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sonoyama, Masashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "21325", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Demura, Makoto", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "21326", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitaku, Shigeki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "21327", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "02-sasaki_et_al_PandP2005.pdf", "filesize": [{"value": "387.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 387500.0, "url": {"label": "02-sasaki_et_al_PandP2005.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/7569/files/02-sasaki_et_al_PandP2005.pdf"}, "version_id": "e79557fb-3ca4-4f52-b840-328b15ce3799"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Photobleaching of Bacteriorhodopsin Solubilized with Triton X-100", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Photobleaching of Bacteriorhodopsin Solubilized with Triton X-100", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["322"], "permalink_uri": "http://hdl.handle.net/2237/9268", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-01-11"}, "publish_date": "2008-01-11", "publish_status": "0", "recid": "7569", "relation": {}, "relation_version_is_last": true, "title": ["Photobleaching of Bacteriorhodopsin Solubilized with Triton X-100"], "weko_shared_id": 3}
  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Photobleaching of Bacteriorhodopsin Solubilized with Triton X-100

http://hdl.handle.net/2237/9268
http://hdl.handle.net/2237/9268
62c08bf3-3e20-4c24-b25f-4e5ce8b32df2
名前 / ファイル ライセンス アクション
02-sasaki_et_al_PandP2005.pdf 02-sasaki_et_al_PandP2005.pdf (387.5 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-01-11
タイトル
タイトル Photobleaching of Bacteriorhodopsin Solubilized with Triton X-100
言語 en
著者 Sasaki, Takanori

× Sasaki, Takanori

WEKO 21324

en Sasaki, Takanori

Search repository
Sonoyama, Masashi

× Sonoyama, Masashi

WEKO 21325

en Sonoyama, Masashi

Search repository
Demura, Makoto

× Demura, Makoto

WEKO 21326

en Demura, Makoto

Search repository
Mitaku, Shigeki

× Mitaku, Shigeki

WEKO 21327

en Mitaku, Shigeki

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 The definitive version is available at www.blackwell-synergy.com
抄録
内容記述 In the current studies, we examined the effects of hexagonal lattice formation with lipid membranes on the structural stability of native bacteriorhodopsin(bR). Denaturation kinetic measurements for bR solubilized with the mild nonionic detergent, Triton X-100(TX100), were performed in the dark and under illumination by visible light. The solubilized bR was stable in the dark over a wide concentration range of TX100(1 to 200 mM). In purple membranes, a bilobed band was observed in visible circular dichroic spectra due to interactions between neighboring chromophores. At all concentrations of TX100, this was replaced by a single positive band. Upon illumination with visible light, TX100-solubilized bR clearly showed photobleaching to bacterioopsin. These experimental results suggest that photobleaching is due to a lack of intermolecular interactions inside the purple membrane lattice. Extensive kinetic measurements further revealed that the rate constant of photobleaching is strongly dependent on the detergent concentration, although the activation energy for photobleaching does not significantly change with the TX100 concentration. The mechanism of photobleaching for the solubilized bR is discussed with respect to detergent micelle properties.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Blackwell
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1562/2005-03-22-RA-470
ISSN
収録物識別子タイプ PISSN
収録物識別子 0031-8655
書誌情報 en : Photochemistry and Photobiology

巻 81, 号 5, p. 1131-1137, 発行日 2005
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/9268
識別子タイプ HDL
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 12:18:08.658822
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3