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Photobleaching of Bacteriorhodopsin Solubilized with Triton X-100
http://hdl.handle.net/2237/9268
http://hdl.handle.net/2237/926862c08bf3-3e20-4c24-b25f-4e5ce8b32df2
名前 / ファイル | ライセンス | アクション |
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02-sasaki_et_al_PandP2005.pdf (387.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-01-11 | |||||
タイトル | ||||||
タイトル | Photobleaching of Bacteriorhodopsin Solubilized with Triton X-100 | |||||
言語 | en | |||||
著者 |
Sasaki, Takanori
× Sasaki, Takanori× Sonoyama, Masashi× Demura, Makoto× Mitaku, Shigeki |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | The definitive version is available at www.blackwell-synergy.com | |||||
抄録 | ||||||
内容記述 | In the current studies, we examined the effects of hexagonal lattice formation with lipid membranes on the structural stability of native bacteriorhodopsin(bR). Denaturation kinetic measurements for bR solubilized with the mild nonionic detergent, Triton X-100(TX100), were performed in the dark and under illumination by visible light. The solubilized bR was stable in the dark over a wide concentration range of TX100(1 to 200 mM). In purple membranes, a bilobed band was observed in visible circular dichroic spectra due to interactions between neighboring chromophores. At all concentrations of TX100, this was replaced by a single positive band. Upon illumination with visible light, TX100-solubilized bR clearly showed photobleaching to bacterioopsin. These experimental results suggest that photobleaching is due to a lack of intermolecular interactions inside the purple membrane lattice. Extensive kinetic measurements further revealed that the rate constant of photobleaching is strongly dependent on the detergent concentration, although the activation energy for photobleaching does not significantly change with the TX100 concentration. The mechanism of photobleaching for the solubilized bR is discussed with respect to detergent micelle properties. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Blackwell | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1562/2005-03-22-RA-470 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0031-8655 | |||||
書誌情報 |
en : Photochemistry and Photobiology 巻 81, 号 5, p. 1131-1137, 発行日 2005 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/9268 | |||||
識別子タイプ | HDL |