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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Bystin in human cancer cells : intracellular localization and function in ribosome biogenesis

http://hdl.handle.net/2237/9306
http://hdl.handle.net/2237/9306
117a733c-51ca-41e2-bc3d-1da07032abb8
名前 / ファイル ライセンス アクション
BJ2007.pdf BJ2007.pdf (2.6 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-01-16
タイトル
タイトル Bystin in human cancer cells : intracellular localization and function in ribosome biogenesis
言語 en
著者 MIYOSHI, Masaya

× MIYOSHI, Masaya

WEKO 21422

en MIYOSHI, Masaya

Search repository
OKAJIMA, Tetsuya

× OKAJIMA, Tetsuya

WEKO 21423

en OKAJIMA, Tetsuya

Search repository
MATSUDA, Tsukasa

× MATSUDA, Tsukasa

WEKO 21424

en MATSUDA, Tsukasa

Search repository
FUKUDA, Michiko N.

× FUKUDA, Michiko N.

WEKO 21425

en FUKUDA, Michiko N.

Search repository
NADANO, Daita

× NADANO, Daita

WEKO 21426

en NADANO, Daita

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 BYSL
キーワード
主題Scheme Other
主題 cancer
キーワード
主題Scheme Other
主題 embryo implantation
キーワード
主題Scheme Other
主題 nucleolar stress
キーワード
主題Scheme Other
主題 ribosomal RNA processing
キーワード
主題Scheme Other
主題 ribosome biogenesis
抄録
内容記述 While bystin has been identified as a protein potentially involved in human embryo implantation-a process unique to mammals-the bystin gene is evolutionarily conserved from yeast to humans. DNA microarray data indicates that bystin is overexpressed in human cancers, suggesting that it promotes cell growth. We undertook RT-PCR and immunoblotting and confirmed that bystin mRNA and protein, respectively, is expressed in human cancer cell lines, including HeLa. Subcellular fractionation identified bystin protein as nuclear and cytoplasmic, and immunofluorescence showed that nuclear bystin localizes mainly in the nucleolus. Sucrose gradient ultracentrifugation of total cytoplasmic ribosomes revealed preferential association of bystin with the 40s subunit fractions. To analyze its function, bystin expression in cells was suppressed by RNA interference. Pulse-chase analysis of ribosomal RNA processing suggested that bystin knockdown delays processing of 18s ribosomal RNA, a component of the 40s submit. Furthermore, this knockdown significantly inhibited cell proliferation. Our findings suggest that bystin may promote cell proliferation by facilitating ribosome biogenesis, specifically in the production of the 40s subunit. Localization of bystin to the nucleolus, the site of ribosome biogenesis, was blocked by low concentrations of actinomycin D, a reagent causing nucleolar stress. when bystin was transiently overexpressed in HeLa cells subjected to nucleolar stress, nuclear bystin was included in particles different from the nuclear stress granules induced by heat shock. By contrast, cytoplasmic bystin was barely affected by nucleolar stress. These results suggest that, while bystin may play multiple roles in mammalian cells, a conserved function is to facilitate ribosome biogenesis required for cell growth.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Biochemical Society
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1042/BJ20061597
書誌情報 en : Biochemical journal

巻 404, 号 3, p. 373-381, 発行日 2007-06
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/9306
識別子タイプ HDL
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