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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Repeated Exposure to Cationic Immunoliposomes Activates Effective Gene Transfer to Human Glioma Cells

http://hdl.handle.net/2237/10864
http://hdl.handle.net/2237/10864
40c59410-fede-42e7-b478-ce94c6b01336
名前 / ファイル ライセンス アクション
mizuno_1214.pdf mizuno_1214.pdf (391.5 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2009-01-22
タイトル
タイトル Repeated Exposure to Cationic Immunoliposomes Activates Effective Gene Transfer to Human Glioma Cells
言語 en
著者 MIZUNO, Masaaki

× MIZUNO, Masaaki

WEKO 25520

en MIZUNO, Masaaki

Search repository
YOSHIDA, Jun

× YOSHIDA, Jun

WEKO 25521

en YOSHIDA, Jun

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 ja
権利情報 日本脳神経外科学会
権利
言語 ja
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
キーワード
主題Scheme Other
主題 cationic immunoliposomes
キーワード
主題Scheme Other
主題 gene transfer
キーワード
主題Scheme Other
主題 repeated exposure
キーワード
主題Scheme Other
主題 glioma
抄録
内容記述 The use of whole immunoglobulin G (IgG) and F(ab')_2 of the G-22 monoclonal antibody associated with cationic liposomes (immunoliposomes) and the effect of repeated exposure were investigated for the transfection of the LacZ gene to various glioma cell lines. Immunoliposomes associated with either whole IgG or F(ab')_2 monoclonal antibody caused an about 2-fold increase in β-galactosidase activity compared with liposomes associated with no antibody in glioma cell lines expressing the CD44 antigen. β-Galactosidase activity was further increased by about 2-fold by repeated exposure compared with single exposure. A glioma cell line not expressing the CD44 antigen showed no such increase in β-galactosidase activity. These results indicate that repeated exposure of cationic immunoliposomes achieves a higher transfection efficiency and is a potentially effective method of gene therapy for patients with malignant glioma. 脳腫瘍に対する遺伝子治療を行なうためには、効率のよい遺伝子導入システムの開発が不可欠である。本論文では遺伝子導入システムのひとつであるカチオニックリポソームに脳腫瘍特異抗体であるG-22モノクローナル抗体を結合させたイムノリポソームの特異性および発現劾率について検討を加えた。その結果、 1) whole IgGとF(ab')_2の違いによる発現効率の差異は認められなかった。2) β-galactosidase遺伝子を導入した実験では抗体結合あるいはリポソームの反復投与により遺伝子発現を増強させることができた。以上の事実よりイムノリポソームの反復投与は導入遺伝子の発現増強につながるものと思われた。
内容記述タイプ Abstract
出版者
言語 ja
出版者 日本脳神経外科学会
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/110002278771/
ISSN
収録物識別子タイプ PISSN
収録物識別子 04708105
書誌情報 en : Neurologia medico-chirurgica

巻 36, 号 3, p. 141-144, 発行日 1996-03
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/10864
識別子タイプ HDL
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