{"_buckets": {"deposit": "3ad0dda3-a122-45ff-872a-f6c0087f7706"}, "_deposit": {"id": "9097", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "9097"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00009097", "sets": ["501"]}, "author_link": ["25520", "25521"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "144", "bibliographicPageStart": "141", "bibliographicVolumeNumber": "36", "bibliographic_titles": [{"bibliographic_title": "Neurologia medico-chirurgica", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The use of whole immunoglobulin G (IgG) and F(ab\u0027)_2 of the G-22 monoclonal antibody associated with cationic liposomes (immunoliposomes) and the effect of repeated exposure were investigated for the transfection of the LacZ gene to various glioma cell lines. Immunoliposomes associated with either whole IgG or F(ab\u0027)_2 monoclonal antibody caused an about 2-fold increase in β-galactosidase activity compared with liposomes associated with no antibody in glioma cell lines expressing the CD44 antigen. β-Galactosidase activity was further increased by about 2-fold by repeated exposure compared with single exposure. A glioma cell line not expressing the CD44 antigen showed no such increase in β-galactosidase activity. These results indicate that repeated exposure of cationic immunoliposomes achieves a higher transfection efficiency and is a potentially effective method of gene therapy for patients with malignant glioma. 脳腫瘍に対する遺伝子治療を行なうためには、効率のよい遺伝子導入システムの開発が不可欠である。本論文では遺伝子導入システムのひとつであるカチオニックリポソームに脳腫瘍特異抗体であるG-22モノクローナル抗体を結合させたイムノリポソームの特異性および発現劾率について検討を加えた。その結果、 1) whole IgGとF(ab\u0027)_2の違いによる発現効率の差異は認められなかった。2) β-galactosidase遺伝子を導入した実験では抗体結合あるいはリポソームの反復投与により遺伝子発現を増強させることができた。以上の事実よりイムノリポソームの反復投与は導入遺伝子の発現増強につながるものと思われた。", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/10864"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本脳神経外科学会", "subitem_publisher_language": "ja"}]}, "item_10_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://ci.nii.ac.jp/naid/110002278771/", "subitem_relation_type_select": "URI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "日本脳神経外科学会", "subitem_rights_language": "ja"}, {"subitem_rights": "本文データは学協会の許諾に基づきCiNiiから複製したものである", "subitem_rights_language": "ja"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "04708105", "subitem_source_identifier_type": "PISSN"}]}, "item_10_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "MIZUNO, Masaaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "25520", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YOSHIDA, Jun", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "25521", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "mizuno_1214.pdf", "filesize": [{"value": "391.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 391500.0, "url": {"label": "mizuno_1214.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/9097/files/mizuno_1214.pdf"}, "version_id": "a1de3ff1-d85e-40b0-962a-eec60f98a1c8"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cationic immunoliposomes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gene transfer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "repeated exposure", "subitem_subject_scheme": "Other"}, {"subitem_subject": "glioma", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Repeated Exposure to Cationic Immunoliposomes Activates Effective Gene Transfer to Human Glioma Cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Repeated Exposure to Cationic Immunoliposomes Activates Effective Gene Transfer to Human Glioma Cells", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["501"], "permalink_uri": "http://hdl.handle.net/2237/10864", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2009-01-22"}, "publish_date": "2009-01-22", "publish_status": "0", "recid": "9097", "relation": {}, "relation_version_is_last": true, "title": ["Repeated Exposure to Cationic Immunoliposomes Activates Effective Gene Transfer to Human Glioma Cells"], "weko_shared_id": -1}