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Immunoliposomes associated with either whole IgG or F(ab\u0027)_2 monoclonal antibody caused an about 2-fold increase in \u03b2-galactosidase activity compared with liposomes associated with no antibody in glioma cell lines expressing the CD44 antigen. \u03b2-Galactosidase activity was further increased by about 2-fold by repeated exposure compared with single exposure. A glioma cell line not expressing the CD44 antigen showed no such increase in \u03b2-galactosidase activity. These results indicate that repeated exposure of cationic immunoliposomes achieves a higher transfection efficiency and is a potentially effective method of gene therapy for patients with malignant glioma. 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