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  1. F100 環境医学研究所
  2. F100a 雑誌掲載論文
  3. 学術雑誌

新生仔ラット培養心筋細胞への高頻度電気刺激負荷によるギャップ結合蛋白発現修飾の検討 : MAPキナーゼとアンギオテンシンII受容体拮抗薬との関連について

http://hdl.handle.net/2237/10933
http://hdl.handle.net/2237/10933
a10e430b-a1c2-4252-81e2-9b351f1cf2b9
名前 / ファイル ライセンス アクション
lee_2090.pdf lee_2090.pdf (629.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2009-01-26
タイトル
タイトル 新生仔ラット培養心筋細胞への高頻度電気刺激負荷によるギャップ結合蛋白発現修飾の検討 : MAPキナーゼとアンギオテンシンII受容体拮抗薬との関連について
言語 ja
その他のタイトル
その他のタイトル Rapid Electrical Stimulation of Contraction Modulates Gap-Junction Protein in Neonatal Rat Cultured Cardiomyocytes Involvement of Mitogen-Activated Protein Kinases and Effects of Angiotensin II-Receptor Antagonist
言語 en
著者 井上, 宣子

× 井上, 宣子

WEKO 25729

ja 井上, 宣子

Search repository
大草, 知子

× 大草, 知子

WEKO 25730

ja 大草, 知子

Search repository
名尾, 朋子

× 名尾, 朋子

WEKO 25731

ja 名尾, 朋子

Search repository
李, 鍾国

× 李, 鍾国

WEKO 25732

ja 李, 鍾国

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松本, 奉

× 松本, 奉

WEKO 25733

ja 松本, 奉

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久松, 裕二

× 久松, 裕二

WEKO 25734

ja 久松, 裕二

Search repository
佐藤, 孝志

× 佐藤, 孝志

WEKO 25735

ja 佐藤, 孝志

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矢野, 雅文

× 矢野, 雅文

WEKO 25736

ja 矢野, 雅文

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安井, 健二

× 安井, 健二

WEKO 25737

ja 安井, 健二

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児玉, 逸雄

× 児玉, 逸雄

WEKO 25738

ja 児玉, 逸雄

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松崎, 益徳

× 松崎, 益徳

WEKO 25739

ja 松崎, 益徳

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INOUE, Noriko

× INOUE, Noriko

WEKO 25740

en INOUE, Noriko

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OHKUSA, Tomoko

× OHKUSA, Tomoko

WEKO 25741

en OHKUSA, Tomoko

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NAO, Tomoko

× NAO, Tomoko

WEKO 25742

en NAO, Tomoko

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LEE, Jong Kook

× LEE, Jong Kook

WEKO 25743

en LEE, Jong Kook

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MATSUMOTO, Tomo

× MATSUMOTO, Tomo

WEKO 25744

en MATSUMOTO, Tomo

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HISAMATSU, Yuji

× HISAMATSU, Yuji

WEKO 25745

en HISAMATSU, Yuji

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SATOH, Takashi

× SATOH, Takashi

WEKO 25746

en SATOH, Takashi

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YANO, Masafumi

× YANO, Masafumi

WEKO 25747

en YANO, Masafumi

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YASUI, Kenji

× YASUI, Kenji

WEKO 25748

en YASUI, Kenji

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KODAMA, Itsuo

× KODAMA, Itsuo

WEKO 25749

en KODAMA, Itsuo

Search repository
MATSUZAKI, Masunori

× MATSUZAKI, Masunori

WEKO 25750

en MATSUZAKI, Masunori

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 ja
権利情報 山口大学医学会
権利
言語 ja
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
キーワード
主題Scheme Other
主題 高頻度刺激
キーワード
主題Scheme Other
主題 コネキシン43
キーワード
主題Scheme Other
主題 伝導速度
キーワード
主題Scheme Other
主題 MAPキナーゼ
キーワード
主題Scheme Other
主題 アンギオテンシンII
抄録
内容記述 心筋細胞間のギャップ結合の発現・分布はコネキシン蛋白の半減期が短いことにより様々な病態において直ちに変化しうる.高頻度電気刺激(RES)によるギャップ結合リモデリングへの効果はいまだ明らかにされていない.培養5日目のラット心室筋細胞に120分間3HzのRESを負荷した.RESによりCx43蛋白質および遺伝子発現量は60分後には有意に増加した.免疫染色においても同様の結果であった.心筋細胞中のangiotensin II (AngII)は15分後に約2倍に上昇した.MAPK系のリン酸化型ERKは2峰性に5分と60分で, またリン酸化型JNKも15分と60分で著明に活性化された.リン酸化型p38 MAPKは5分後に1峰性に活性化された.細胞外電位記録法により心筋細胞の興奮伝播特性の変化を解析したところ, RESにより伝導速度は有意に増加した.これらの変化はlosartanにより抑制された.RESによるCx43の発現増加はまたERK, p38の特異的阻害剤にても抑制された.RESは, 早期より心筋細胞内のAng II産生を増加し, MAPK系を活性化することによりCx43発現量を増加させた.その結果, 細胞間の刺激伝播異常を引き起こし, 不整脈基質の一つとなる可能性が示された.
言語 ja
内容記述タイプ Abstract
抄録
内容記述 BACKGROUND The expression and distribution of gap junctions in cardiac muscle can be changed readily under a variety of pathological conditions because of dynamic turnover of connexins. The effects of rapid electrical stimulation (RES) of contraction on the gap junction remodeling are not well-understood. METHODS Neonatal rat ventricular myocytes cultured for 5 days were subjected to RES at 3.0 Hz for up to 120 min. RESULTS RES resulted in a significant upregulation of Cx43. Angiotensin II content was increased significantly by RES>15 min. Phosphorylated forms of extracellular signal-regulated protein kinase (ERK), c-Jun NH_2-terminal kinases (JNK) and p38 mitogen-activated protein kinases (p38 MAPKs) were all increased dramatically by RES with peaks at 5~60 min. Propagation of excitation was visualized by extracellular potential mapping by using a multiple electrode array system. Conduction velocity was increased significantly by RES for 60 to 90 min. Treatment of myocytes with losartan prevented most of these effects of RES. RES-induced upregulation of Cx43 was also prevented by specific inhibitors for ERK and p38 MAPKs. CONCLUSIONS A short-term RES causes upregulation of Cx43 in cardiomyocytes and a concomitant increase of conduction velocity mainly through an autocrine action of Angiotensin II to activate ERK and p38 MAPKs.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 (小西賞受賞者)(Konishi Prize)
言語 ja
内容記述タイプ Other
出版者
言語 ja
出版者 山口大学医学会
言語
言語 jpn
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/110002774473/
ISSN
収録物識別子タイプ PISSN
収録物識別子 05131731
書誌情報 ja : 山口医学

巻 54, 号 4, p. 109-115, 発行日 2005-08
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/10933
識別子タイプ HDL
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