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  1. C100 医学部/医学系研究科
  2. C100d 学位論文
  3. 博士論文(医保博・論医保博)

Uniaxial Cyclic Stretch-Stimulated Glucose Transport Is Mediated by a Ca2+-Dependent Mechanism in Cultured Skeletal Muscle Cells

http://hdl.handle.net/2237/11109
http://hdl.handle.net/2237/11109
147a0d5c-9dcf-4595-b0f8-45021976e142
名前 / ファイル ライセンス アクション
MIwata_text_mod_final_figure.pdf MIwata_text_mod_final_figure.pdf (1.7 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2009-02-13
タイトル
タイトル Uniaxial Cyclic Stretch-Stimulated Glucose Transport Is Mediated by a Ca2+-Dependent Mechanism in Cultured Skeletal Muscle Cells
言語 en
著者 岩田, 全広

× 岩田, 全広

WEKO 26659

ja 岩田, 全広

Search repository
Iwata, Masahiro

× Iwata, Masahiro

WEKO 26660

en Iwata, Masahiro

Search repository
Hayakawa, Kimihide

× Hayakawa, Kimihide

WEKO 26661

en Hayakawa, Kimihide

Search repository
Murakami, Taro

× Murakami, Taro

WEKO 26662

en Murakami, Taro

Search repository
Naruse, Keiji

× Naruse, Keiji

WEKO 26663

en Naruse, Keiji

Search repository
Kawakami, Keisuke

× Kawakami, Keisuke

WEKO 26664

en Kawakami, Keisuke

Search repository
Inoue-Miyazu, Masumi

× Inoue-Miyazu, Masumi

WEKO 26665

en Inoue-Miyazu, Masumi

Search repository
Yuge, Louis

× Yuge, Louis

WEKO 26666

en Yuge, Louis

Search repository
Suzuki, Shigeyuki

× Suzuki, Shigeyuki

WEKO 26667

en Suzuki, Shigeyuki

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 Mechanical stretch
キーワード
主題Scheme Other
主題 Myotube
キーワード
主題Scheme Other
主題 Glucose uptake
キーワード
主題Scheme Other
主題 Intracellular Ca 2+ concentration
抄録
内容記述 Objective: Mechanical stimuli such as stretch increase glucose transport and glycogen metabolism in skeletal muscle. However, the molecular mechanisms involved in the mechanotransduction events are poorly understood. The present study was conducted in order to determine whether the signaling mechanism leading to mechanical stretch-stimulated glucose transport is similar to, or distinct from, the signaling mechanisms leading to insulin- and contraction-stimulated glucose transport in cultured muscle cells. Methods: Cultured C2C12 myotubes were stretched, after which the 2-deoxy-D-glucose (2-DG) uptake was measured. Results: Following cyclic stretch, C2C12 myotubes showed a significant increase in 2-DG uptake, and this effect was not prevented by inhibiting phosphatidylinositol 3-kinase or 5'-AMP-activated protein kinase and by extracellular Ca2+ chelation. Conversely, the stretch-stimulated 2-DG uptake was completely prevented by dantrolene (an inhibitor of Ca2+ release from sarcoplasmic reticulum). Furthermore, the stretch-stimulated 2-DG uptake was prevented by the Ca2+/calmodulin-dependent kinase inhibitor KN93 which did not prevent the insulin-stimulated 2-DG uptake. Conclusions: These results suggest that the effects of stretch-stimulated glucose transport are independent of the insulin-signaling pathway. By contrast, following mechanical stretch in skeletal muscle, the signal transduction pathway leading to glucose transport may require the participation of cytosolic Ca2+ and Ca2+/calmodulin kinase, but not 5'-AMP-activated protein kinase.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 名古屋大学博士学位論文 学位の種類:博士(リハビリテーション療法学)(課程)学位授与年月日:平成19年3月23日
言語 ja
内容記述タイプ Other
内容記述
内容記述 Uniaxial Cyclic Stretch-Stimulated Glucose Transport Is Mediated by a Ca2+-Dependent Mechanism in Cultured Skeletal Muscle Cells Pathobiology, v.74, n.3, pp.159-168を、博士論文として提出したもの。
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Karger
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1159/000103375
ISSN
収録物識別子タイプ PISSN
収録物識別子 1015-2008
書誌情報 en : Pathobiology

巻 74, 号 3, p. 159-168, 発行日 2007-07
学位名
言語 ja
学位名 博士(リハビリテーション療法学)
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 13901
言語 ja
学位授与機関名 名古屋大学
言語 en
学位授与機関名 NAGOYA University
学位授与年度
学位授与年度 2007
学位授与年月日
学位授与年月日 2007-07-01
学位授与番号
学位授与番号 甲第7284号
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/11109
識別子タイプ HDL
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