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  1. B100 理学部/理学研究科
  2. B100d 学位論文
  3. 博士論文(理博・論理博)

特異的阻害剤フェミナル耐性変異体を用いたNa[+]駆動型べん毛モ-タ-のエネルギ-変換ユニットの研究

http://hdl.handle.net/2237/11214
http://hdl.handle.net/2237/11214
68c74f94-ecef-4576-b81e-00896ba00dd4
名前 / ファイル ライセンス アクション
ko4179.pdf ko4179.pdf (3.7 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2009-02-27
タイトル
タイトル 特異的阻害剤フェミナル耐性変異体を用いたNa[+]駆動型べん毛モ-タ-のエネルギ-変換ユニットの研究
言語 ja
著者 小嶋, 誠司

× 小嶋, 誠司

WEKO 26934

ja 小嶋, 誠司

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
抄録
内容記述 Bacterial flagellar motor is the tiny and elaborate molecular machine which converts the electrochemical gradient of a specific ion (H^+ or Na^+) across the cytoplasmic membrane into the mechanical work. To understand the mechanism of force generation of the flagellar motor, it is essential to know how the coupling ion interacts with the force-generating unit of the motor. In this study, I investigated ion coupling sites of the Na^+ -driven motor by using the motility mutants resistant to a specific inhibitor, phenamil, whose interaction sites are thought to be located near Na^+ binding sites in force-generating units, and provided the first molecular evidence that phenamil directly interacts with the Na^+ channel components (PomA and PomB) of the motor. I isolated motility mutants resistant to phenamil from the marine bacterium Vibrio alginolyticus, whose polar flagella rotate by Na^+ - driven motors, and named the phenotype Mpa^r for motility resistant to phenamil. The motility of the wild type (Mpa^s) was inhibited by 50μM phenamil, whereas Mpa^r strains were still motile in the presence of 200μM phenamil. The Ki value for phenamil in the Mpa^r strain was estimated to be 5 times larger than that in the Mpa^s strain. However, the sensitivities to amiloride or bezamil, another amiloride analog, were not distinctly changed in the Mpa^r strain. The rotation rate of the wild-type Na^+ -driven motor fluctuates greatly in the presence of phenamil, which can be explained in terms of a relatively slow dissociation rate of phenamil from the motor. I therefore studied the stability of the rotation of the Mpa^r and Mpa^s motors by phenamil. The speed fluctuations of the Mpa^r motors were distinctly reduced relative to the Mpa^s motors. The steadier rotation of the Mpa^r motors can be explained by an increase in the phenamil dissociation rate from a sodium channel of the motor, which suggests that a phenamil-specific binding site of the motor is mutated in the Mpa^r strain. Based on these results, I expected that the Mpa^r mutations would be mapped in the genes encoding channel components of the Na^+ driven motor, such as pom (polar-flagellar motility) genes. I cloned and sequenced pom A and pomB from two Mpa^r strains, NMB205 and NMB201, and found a substitution in pom A (Asp148 to Tyr; NMB205) and in pom B (Pro16 to Ser; NMB201). Both residues are predicted to be near the cytoplasmic ends of the putative transmembrane segments. Mutational analyses at Pom A-Asp148 and PomB-Pro16 suggest that a certain structural change around these residues affects the sensitivity of the motor to phenamil. Co-expression of the PomA D148Y and PomB P16S proteins resulted in an Mpa^r phenotype which seemed to be less sensitive to phenamil than either of the single mutants, although motility was more severely impaired in the absence of inhibitors. These results support the idea that PomA and PomB interact with each other and suggest that multiple residues, including Asp148 of PomA and Pro16 of PomB, constitute a high-affinity phenamil-binding site at the inner face of the PomA/PomB channel complex.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 名古屋大学博士学位論文 学位の種類:博士(理学) (課程) 学位授与年月日:平成11年2月26日
言語 ja
内容記述タイプ Other
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
書誌情報
発行日 1999-02-26
学位名
言語 ja
学位名 博士(理学)
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 13901
言語 ja
学位授与機関名 名古屋大学
言語 en
学位授与機関名 Nagoya University
学位授与年度
学位授与年度 1998
学位授与年月日
学位授与年月日 1999-02-26
学位授与番号
学位授与番号 甲第4179号
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/11214
識別子タイプ HDL
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