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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Diazotization of kynurenine by acidified nitrite secreted from indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells

http://hdl.handle.net/2237/11379
http://hdl.handle.net/2237/11379
606d5319-6668-4cad-9594-d6a19fa4522d
名前 / ファイル ライセンス アクション
Diazo-1.pdf Diazo-1.pdf (305.8 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2009-04-01
タイトル
タイトル Diazotization of kynurenine by acidified nitrite secreted from indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells
言語 en
著者 Hara, Toshiaki

× Hara, Toshiaki

WEKO 29158

en Hara, Toshiaki

Search repository
Yamakura, Fumiyuki

× Yamakura, Fumiyuki

WEKO 29159

en Yamakura, Fumiyuki

Search repository
Takikawa, Osamu

× Takikawa, Osamu

WEKO 29160

en Takikawa, Osamu

Search repository
Hiramatsu, Rie

× Hiramatsu, Rie

WEKO 29161

en Hiramatsu, Rie

Search repository
Kawabe, Tsutomu

× Kawabe, Tsutomu

WEKO 29162

en Kawabe, Tsutomu

Search repository
Isobe, Ken-ichi

× Isobe, Ken-ichi

WEKO 29163

en Isobe, Ken-ichi

Search repository
Nagase, Fumihiko

× Nagase, Fumihiko

WEKO 29164

en Nagase, Fumihiko

Search repository
長瀬, 文彦

× 長瀬, 文彦

WEKO 29165

ja 長瀬, 文彦

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 Bone marrow-derived myeloid dendritic cells
キーワード
主題Scheme Other
主題 Indoleamine 2,3-dioxygenase
キーワード
主題Scheme Other
主題 Tryptophan
キーワード
主題Scheme Other
主題 Kynurenine
キーワード
主題Scheme Other
主題 Nitric oxide
キーワード
主題Scheme Other
主題 Diazotization
抄録
内容記述 Indoleamine 2,3-dioxygenase (IDO)-initiated tryptophan metabolism along the kynurenine (Kyn) pathway regulates T-cell responses in some dendritic cells (DC) such as plasmacytoid DC. A Kyn assay using HPLC showed that samples were frequently deproteinized with trichloroacetic acid (TCA). In the present study, bone marrow derived myeloid DC (BMDC) were differentiated from mouse bone marrow cells with GM-CSF. CpG oligodeoxynucleotides (CpG) induced the expression of IDO protein with NO production in BMDC cultured for 24 hr. The concentrations of Kyn in the culture supernatants were not increased by stimulation with CpG but rather decreased by based on the Kyn assay after deproteinization with TCA. The level of Kyn exogenously added into the cell-free culture supernatant of BMDC stimulated with CpG was severely decreased by deproteinization with TCA but not methanol, and the decrease was prevented when BMDC was stimulated with CpG in the presence of a NOS inhibitor. Under acidic conditions, Kyn reacted with nitrite produced by BMDC, and generated a new compound that was not detected by Ehrlich reagent reacting with the aromatic amino residue of Kyn. An analysis by mass spectrometry showed the new compound to be a diazotization form of Kyn. In conclusion, the deproteinization of samples by acidic treatment should be avoided for the Kyn assay when NO is produced.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jim.2007.11.010
ISSN
収録物識別子タイプ PISSN
収録物識別子 0022-1759
書誌情報 en : Journal of Immunological Methods

巻 332, p. 162-169, 発行日 2008-03
フォーマット
application/pdf
著者版フラグ
値 author
URI
識別子 http://hdl.handle.net/2237/11379
識別子タイプ HDL
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