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Diazotization of kynurenine by acidified nitrite secreted from indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells
http://hdl.handle.net/2237/11379
http://hdl.handle.net/2237/11379606d5319-6668-4cad-9594-d6a19fa4522d
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-04-01 | |||||
タイトル | ||||||
タイトル | Diazotization of kynurenine by acidified nitrite secreted from indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells | |||||
言語 | en | |||||
著者 |
Hara, Toshiaki
× Hara, Toshiaki× Yamakura, Fumiyuki× Takikawa, Osamu× Hiramatsu, Rie× Kawabe, Tsutomu× Isobe, Ken-ichi× Nagase, Fumihiko× 長瀬, 文彦 |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone marrow-derived myeloid dendritic cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Indoleamine 2,3-dioxygenase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tryptophan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Kynurenine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nitric oxide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Diazotization | |||||
抄録 | ||||||
内容記述 | Indoleamine 2,3-dioxygenase (IDO)-initiated tryptophan metabolism along the kynurenine (Kyn) pathway regulates T-cell responses in some dendritic cells (DC) such as plasmacytoid DC. A Kyn assay using HPLC showed that samples were frequently deproteinized with trichloroacetic acid (TCA). In the present study, bone marrow derived myeloid DC (BMDC) were differentiated from mouse bone marrow cells with GM-CSF. CpG oligodeoxynucleotides (CpG) induced the expression of IDO protein with NO production in BMDC cultured for 24 hr. The concentrations of Kyn in the culture supernatants were not increased by stimulation with CpG but rather decreased by based on the Kyn assay after deproteinization with TCA. The level of Kyn exogenously added into the cell-free culture supernatant of BMDC stimulated with CpG was severely decreased by deproteinization with TCA but not methanol, and the decrease was prevented when BMDC was stimulated with CpG in the presence of a NOS inhibitor. Under acidic conditions, Kyn reacted with nitrite produced by BMDC, and generated a new compound that was not detected by Ehrlich reagent reacting with the aromatic amino residue of Kyn. An analysis by mass spectrometry showed the new compound to be a diazotization form of Kyn. In conclusion, the deproteinization of samples by acidic treatment should be avoided for the Kyn assay when NO is produced. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Elsevier | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.jim.2007.11.010 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0022-1759 | |||||
書誌情報 |
en : Journal of Immunological Methods 巻 332, p. 162-169, 発行日 2008-03 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/11379 | |||||
識別子タイプ | HDL |