{"_buckets": {"deposit": "f5a5c63d-eb6c-48c2-b29a-e887ade6a359"}, "_deposit": {"id": "9582", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "9582"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00009582"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "169", "bibliographicPageStart": "162", "bibliographicVolumeNumber": "332", "bibliographic_titles": [{"bibliographic_title": "Journal of Immunological Methods"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Indoleamine 2,3-dioxygenase (IDO)-initiated tryptophan metabolism along the\nkynurenine (Kyn) pathway regulates T-cell responses in some dendritic cells (DC) such\nas plasmacytoid DC. A Kyn assay using HPLC showed that samples were frequently\ndeproteinized with trichloroacetic acid (TCA). In the present study, bone marrow\nderived myeloid DC (BMDC) were differentiated from mouse bone marrow cells with\nGM-CSF. CpG oligodeoxynucleotides (CpG) induced the expression of IDO protein\nwith NO production in BMDC cultured for 24 hr. The concentrations of Kyn in the\nculture supernatants were not increased by stimulation with CpG but rather decreased\nby based on the Kyn assay after deproteinization with TCA. The level of Kyn\nexogenously added into the cell-free culture supernatant of BMDC stimulated with CpG\nwas severely decreased by deproteinization with TCA but not methanol, and the\ndecrease was prevented when BMDC was stimulated with CpG in the presence of a\nNOS inhibitor. Under acidic conditions, Kyn reacted with nitrite produced by BMDC,\nand generated a new compound that was not detected by Ehrlich reagent reacting with\nthe aromatic amino residue of Kyn. An analysis by mass spectrometry showed the\nnew compound to be a diazotization form of Kyn. In conclusion, the deproteinization\nof samples by acidic treatment should be avoided for the Kyn assay when NO is\nproduced.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/11379"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0022-1759", "subitem_source_identifier_type": "ISSN"}]}, "item_10_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hara, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "29158", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamakura, Fumiyuki"}], "nameIdentifiers": [{"nameIdentifier": "29159", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takikawa, Osamu"}], "nameIdentifiers": [{"nameIdentifier": "29160", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hiramatsu, Rie"}], "nameIdentifiers": [{"nameIdentifier": "29161", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawabe, Tsutomu"}], "nameIdentifiers": [{"nameIdentifier": "29162", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Isobe, Ken-ichi"}], "nameIdentifiers": [{"nameIdentifier": "29163", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagase, Fumihiko"}], "nameIdentifiers": [{"nameIdentifier": "29164", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u9577\u702c, \u6587\u5f66"}], "nameIdentifiers": [{"nameIdentifier": "29165", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Diazo-1.pdf", "filesize": [{"value": "305.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 305800.0, "url": {"label": "Diazo-1.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/9582/files/Diazo-1.pdf"}, "version_id": "6c849d33-71dc-4c58-b78a-ca00effc86dd"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Bone marrow-derived myeloid dendritic cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Indoleamine 2,3-dioxygenase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tryptophan", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Kynurenine", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Nitric oxide", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Diazotization", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Diazotization of kynurenine by acidified nitrite secreted from indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Diazotization of kynurenine by acidified nitrite secreted from indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells"}]}, "item_type_id": "10", "owner": "1", "path": ["499/500/501"], "permalink_uri": "http://hdl.handle.net/2237/11379", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2009-04-01"}, "publish_date": "2009-04-01", "publish_status": "0", "recid": "9582", "relation": {}, "relation_version_is_last": true, "title": ["Diazotization of kynurenine by acidified nitrite secreted from indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells"], "weko_shared_id": null}