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High-affinity uptake of kynurenine and nitric oxide-mediated inhibition of indoleamine 2,3-dioxygenase in bone marrow-derived myeloid dendritic cells
http://hdl.handle.net/2237/11381
http://hdl.handle.net/2237/113812a5c1be5-91ba-4d69-8a11-4616cb2b476d
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-04-01 | |||||
タイトル | ||||||
タイトル | High-affinity uptake of kynurenine and nitric oxide-mediated inhibition of indoleamine 2,3-dioxygenase in bone marrow-derived myeloid dendritic cells | |||||
言語 | en | |||||
著者 |
Hara, Toshiaki
× Hara, Toshiaki× Ogasawara, Nanako× Akimoto, Hidetoshi× Takikawa, Osamu× Hiramatsu, Rie× Kawabe, Tsutomu× Isobe, Ken-ichi× Nagase, Fumihiko× 長瀬, 文彦 |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone marrow-derived myeloid dendritic cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Indoleamine 2,3-dioxygenase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tryptophan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Kynurenine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nitric oxide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transport system L | |||||
抄録 | ||||||
内容記述 | Indoleamine 2,3-dioxygenase (IDO)-initiated tryptophan metabolism along the kynurenine (Kyn) pathway in some dendritic cells (DC) such as plasmacytoid DC regulates T-cell responses. It is unclear whether bone marrow-derived myeloid DC (BMDC) express functional IDO. The IDO expression was examined in CD11c+CD11b+ BMDC differentiated from mouse bone marrow cells using GM-CSF. CpG oligodeoxynucleotides (CpG) induced the expression of IDO protein with the production of nitric oxide (NO) in BMDC in cultures for 24 hr. In the enzyme assay using cellular extracts of BMDC, the IDO activity of BMDC stimulated with CpG was enhanced by the addition of a NO synthase (NOS) inhibitor, suggesting that IDO activity was suppressed by NO production. On the other hand, the concentration of Kyn in the culture supernatant of BMDC was not increased by stimulation with CpG. Exogenously added Kyn was taken up by BMDC independently of CpG stimulation and NO production, and the uptake of Kyn was inhibited by a transport system L-specific inhibitor or high concentrations of tryptophan. The uptake of tryptophan by BMDC was markedly lower than that of Kyn. In conclusion, IDO activity in BMDC is down-regulated by NO production, whereas BMDC strongly take up exogenous Kyn. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Elsevier | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.imlet.2007.11.016 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 01652478 | |||||
書誌情報 |
en : Immunology Letters 巻 116, p. 95-102, 発行日 2008-02-15 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/11381 | |||||
識別子タイプ | HDL |